Fitness for 5 genes in Escherichia coli BW25113

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500 ntyjhU and yjhF are separated by 346 nucleotidesyjhF and yjhG are separated by 106 nucleotidesyjhG and yjhH are separated by 10 nucleotidesyjhH and yjhI are separated by 4 nucleotides b4295: yjhU - orf, hypothetical protein (VIMSS), at 4,517,361 to 4,518,347 yjhU b4296: yjhF - KpLE2 phage-like element; predicted transporter (NCBI), at 4,518,694 to 4,520,043 yjhF b4297: yjhG - KpLE2 phage-like element; predicted dehydratase (NCBI), at 4,520,150 to 4,522,117 yjhG b4298: yjhH - putative lyase/synthase (VIMSS), at 4,522,128 to 4,523,033 yjhH b4299: yjhI - KpLE2 phage-like element; predicted DNA-binding transcriptional regulator (NCBI), at 4,523,038 to 4,523,826 yjhI
Group Condition b4295 b4296 b4297 b4298 b4299
yjhU yjhF yjhG yjhH yjhI
stress LB Aerobic with Ramoplanin 0.000125 mM -0.4 -0.4 -0.0 -0.2 -0.5
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.3 -0.4 -0.0 -0.2 -0.4
motility outer cut, LB soft agar motility assay -0.4 -0.3 -0.1 -0.2 -0.2
predation 3 hr rep1_Tiberius_no_dilution -0.0 -0.2 -0.0 -0.1 -0.8
predation 3 hr rep1_Tiberius_10fold_dilution +0.1 -0.3 -0.0 -0.2 -0.8
stress LB Anaerobic with Irgasan 0.000000625 mM -0.1 -0.3 -0.1 -0.2 -0.5
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.1 -0.4 +0.3 -0.8 -0.4
carbon source carbon source D-Glucose 0.25mM -0.0 +0.2 +0.3 +0.1 -1.6
M9 Acetate 0.5% aerobic +0.1 -0.2 -0.0 -0.0 -0.9
stress LB Aerobic with Ramoplanin 0.000125 mM -0.2 -0.3 +0.1 +0.1 -0.6
nopredatorcontrol 3 hr NoPredatorControl +0.1 -0.2 -0.0 +0.2 -0.9
predation 3 hr rep1_HD100_100fold_dilution +0.2 -0.3 -0.1 -0.1 -0.6
predation 3 hr rep1_HD100_100fold_dilution +0.1 -0.3 -0.1 +0.2 -0.7
stress LB Anaerobic with 2-Mercaptopyridine N-oxide 0.000015625 mM +0.1 -0.1 +0.1 -0.4 -0.4
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.4 -0.1 -0.2 -0.5 -0.2
nopredatorcontrol 3 hr NoPredatorControl +0.3 -0.2 +0.0 -0.1 -0.6
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.4 -0.0 -0.1 -0.0 -0.7
phage Ste41 +0.6 -0.4 +0.5 -0.9 -0.3
stress LB Anaerobic with Spiramycin 0.00003125 mM +0.3 -0.0 -0.0 +0.1 -0.8
predation 3 hr rep1_Tiberius_100fold_dilution +0.4 -0.3 +0.0 +0.1 -0.6
stress LB Aerobic with Spiramycin 0.000125 mM +0.2 -0.0 +0.0 +0.3 -0.7
carbon source carbon source D-Mannose 1mM -0.2 +0.3 -0.3 -0.3 +0.3
stress LB Aerobic with Irgasan 0.0000003125 mM -0.5 +0.0 -0.2 -0.2 +0.7
phage RB68 +0.5 -0.1 -0.0 -0.3 -0.2
control PBS_cage_1_mouse_1_Cecum_40 hr -0.3 +0.2 +0.2 +0.2 -0.3
stress Cephalothin 0.01 mg/ml -0.4 +0.1 +0.1 +0.2 +0.5
phage Papa dilution 10-1 cacl2 maltose +0.0 +0.2 -0.3 -0.2 +0.9
phage T7_phage 0.00015625 MOI TC3 +0.3 +0.2 +0.3 +0.1 -0.3
control PBS_cage_2_mouse_2_Cecum_40 hr +0.1 +0.3 -0.1 +0.3 +0.5
stress M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM +0.2 +0.3 +0.1 +0.0 +0.7
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