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Protein
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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yjhU and yjhF are separated by 346 nucleotides
yjhF and yjhG are separated by 106 nucleotides
yjhG and yjhH are separated by 10 nucleotides
yjhH and yjhI are separated by 4 nucleotides
b4295: yjhU - orf, hypothetical protein (VIMSS), at 4,517,361 to 4,518,347
yjhU
b4296: yjhF - KpLE2 phage-like element; predicted transporter (NCBI), at 4,518,694 to 4,520,043
yjhF
b4297: yjhG - KpLE2 phage-like element; predicted dehydratase (NCBI), at 4,520,150 to 4,522,117
yjhG
b4298: yjhH - putative lyase/synthase (VIMSS), at 4,522,128 to 4,523,033
yjhH
b4299: yjhI - KpLE2 phage-like element; predicted DNA-binding transcriptional regulator (NCBI), at 4,523,038 to 4,523,826
yjhI
Group
Condition
b4295
b4296
b4297
b4298
b4299
yjhU
yjhF
yjhG
yjhH
yjhI
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.4
-0.4
-0.0
-0.2
-0.5
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.3
-0.4
-0.0
-0.2
-0.4
motility
outer cut, LB soft agar motility assay
-0.4
-0.3
-0.1
-0.2
-0.2
predation
3 hr rep1_Tiberius_no_dilution
-0.0
-0.2
-0.0
-0.1
-0.8
predation
3 hr rep1_Tiberius_10fold_dilution
+0.1
-0.3
-0.0
-0.2
-0.8
stress
LB Anaerobic with Irgasan 0.000000625 mM
-0.1
-0.3
-0.1
-0.2
-0.5
predation
6 hr rep1_HD100_10fold_dilution_grewON
+0.1
-0.4
+0.3
-0.8
-0.4
carbon source
carbon source D-Glucose 0.25mM
-0.0
+0.2
+0.3
+0.1
-1.6
M9 Acetate 0.5% aerobic
+0.1
-0.2
-0.0
-0.0
-0.9
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.2
-0.3
+0.1
+0.1
-0.6
nopredatorcontrol
3 hr NoPredatorControl
+0.1
-0.2
-0.0
+0.2
-0.9
predation
3 hr rep1_HD100_100fold_dilution
+0.2
-0.3
-0.1
-0.1
-0.6
predation
3 hr rep1_HD100_100fold_dilution
+0.1
-0.3
-0.1
+0.2
-0.7
stress
LB Anaerobic with 2-Mercaptopyridine N-oxide 0.000015625 mM
+0.1
-0.1
+0.1
-0.4
-0.4
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.4
-0.1
-0.2
-0.5
-0.2
nopredatorcontrol
3 hr NoPredatorControl
+0.3
-0.2
+0.0
-0.1
-0.6
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
+0.4
-0.0
-0.1
-0.0
-0.7
phage
Ste41
+0.6
-0.4
+0.5
-0.9
-0.3
stress
LB Anaerobic with Spiramycin 0.00003125 mM
+0.3
-0.0
-0.0
+0.1
-0.8
predation
3 hr rep1_Tiberius_100fold_dilution
+0.4
-0.3
+0.0
+0.1
-0.6
stress
LB Aerobic with Spiramycin 0.000125 mM
+0.2
-0.0
+0.0
+0.3
-0.7
carbon source
carbon source D-Mannose 1mM
-0.2
+0.3
-0.3
-0.3
+0.3
stress
LB Aerobic with Irgasan 0.0000003125 mM
-0.5
+0.0
-0.2
-0.2
+0.7
phage
RB68
+0.5
-0.1
-0.0
-0.3
-0.2
control
PBS_cage_1_mouse_1_Cecum_40 hr
-0.3
+0.2
+0.2
+0.2
-0.3
stress
Cephalothin 0.01 mg/ml
-0.4
+0.1
+0.1
+0.2
+0.5
phage
Papa dilution 10-1 cacl2 maltose
+0.0
+0.2
-0.3
-0.2
+0.9
phage
T7_phage 0.00015625 MOI TC3
+0.3
+0.2
+0.3
+0.1
-0.3
control
PBS_cage_2_mouse_2_Cecum_40 hr
+0.1
+0.3
-0.1
+0.3
+0.5
stress
M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM
+0.2
+0.3
+0.1
+0.0
+0.7
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