Fitness for 5 genes in Escherichia coli BW25113

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500 ntvalS and holC overlap by 1 nucleotidesholC and pepA are separated by 159 nucleotidespepA and yjgP are separated by 266 nucleotidesyjgP and yjgQ overlap by 1 nucleotides b4258: valS - valyl-tRNA synthetase (NCBI), at 4,479,005 to 4,481,860 valS b4259: holC - DNA polymerase III subunit chi (NCBI), at 4,481,860 to 4,482,303 holC b4260: pepA - leucyl aminopeptidase (NCBI), at 4,482,463 to 4,483,974 pepA b4261: yjgP - conserved inner membrane protein (NCBI), at 4,484,241 to 4,485,341 yjgP b4262: yjgQ - orf, hypothetical protein (VIMSS), at 4,485,341 to 4,486,423 yjgQ
Group Condition b4258 b4259 b4260 b4261 b4262
valS holC pepA yjgP yjgQ
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM N.D. N.D. -1.8 N.D. N.D.
stress 2,4-Dinitrophenol 2 mM N.D. N.D. -1.7 N.D. N.D.
carbon source Glycolic Acid (C) N.D. N.D. -1.7 N.D. N.D.
carbon source Glycolic Acid (C) N.D. N.D. -1.6 N.D. N.D.
stress LB Anaerobic with Chloramphenicol 0.00000390625 mM N.D. N.D. -1.5 N.D. N.D.
stress LB Anaerobic with Furaltadone 0.000015625 mM N.D. N.D. -1.4 N.D. N.D.
stress LB Anaerobic with Chloramphenicol 0.000001953125 mM N.D. N.D. -1.3 N.D. N.D.
stress 2,4-Dinitrophenol 2 mM N.D. N.D. -1.3 N.D. N.D.
retron_ec_48_control retron_Ec_48_control N.D. N.D. -1.2 N.D. N.D.
stress LB Anaerobic with Furaltadone 0.000015625 mM N.D. N.D. -1.2 N.D. N.D.
stress LB Anaerobic with Chloramphenicol 0.00000390625 mM N.D. N.D. -1.2 N.D. N.D.
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM N.D. N.D. -1.2 N.D. N.D.
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM N.D. N.D. -1.2 N.D. N.D.
mzaabcde mzaABCDE N.D. N.D. -1.1 N.D. N.D.
mzaabcde mzaABCDE N.D. N.D. -1.1 N.D. N.D.
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM N.D. N.D. -1.0 N.D. N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. N.D. -1.0 N.D. N.D.
carbon source Ying_Nucleobase14 rep C; time point 1 N.D. N.D. +1.0 N.D. N.D.
carbon source Ying_Nucleobase14 rep A; time point 1 N.D. N.D. +1.0 N.D. N.D.
carbon source carbon source D-Glucose 0.25mM N.D. N.D. +1.0 N.D. N.D.
stress Cholin acetate 75 mM N.D. N.D. +1.1 N.D. N.D.
stress acetate 70 mM N.D. N.D. +1.2 N.D. N.D.
stress Cholin acetate 150 mM N.D. N.D. +1.4 N.D. N.D.
stress acetate 140 mM N.D. N.D. +1.4 N.D. N.D.
carbon source Ying_Nucleobase14 rep C; time point 2 N.D. N.D. +1.7 N.D. N.D.
carbon source Ying_Nucleobase14 rep B; time point 2 N.D. N.D. +1.7 N.D. N.D.
carbon source Ying_Nucleobase14 rep A; time point 2 N.D. N.D. +1.8 N.D. N.D.
carbon source Ying_Nucleobase14 rep A; time point 3 N.D. N.D. +2.6 N.D. N.D.
carbon source Ying_Nucleobase14 rep C; time point 3 N.D. N.D. +2.6 N.D. N.D.
carbon source Ying_Nucleobase14 rep B; time point 3 N.D. N.D. +2.7 N.D. N.D.
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