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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
valS and holC overlap by 1 nucleotides
holC and pepA are separated by 159 nucleotides
pepA and yjgP are separated by 266 nucleotides
yjgP and yjgQ overlap by 1 nucleotides
b4258: valS - valyl-tRNA synthetase (NCBI), at 4,479,005 to 4,481,860
valS
b4259: holC - DNA polymerase III subunit chi (NCBI), at 4,481,860 to 4,482,303
holC
b4260: pepA - leucyl aminopeptidase (NCBI), at 4,482,463 to 4,483,974
pepA
b4261: yjgP - conserved inner membrane protein (NCBI), at 4,484,241 to 4,485,341
yjgP
b4262: yjgQ - orf, hypothetical protein (VIMSS), at 4,485,341 to 4,486,423
yjgQ
Group
Condition
b4258
b4259
b4260
b4261
b4262
valS
holC
pepA
yjgP
yjgQ
stress
LB Anaerobic with Nitrofurantoin 0.000015625 mM
N.D.
N.D.
-1.8
N.D.
N.D.
stress
2,4-Dinitrophenol 2 mM
N.D.
N.D.
-1.7
N.D.
N.D.
carbon source
Glycolic Acid (C)
N.D.
N.D.
-1.7
N.D.
N.D.
carbon source
Glycolic Acid (C)
N.D.
N.D.
-1.6
N.D.
N.D.
stress
LB Anaerobic with Chloramphenicol 0.00000390625 mM
N.D.
N.D.
-1.5
N.D.
N.D.
stress
LB Anaerobic with Furaltadone 0.000015625 mM
N.D.
N.D.
-1.4
N.D.
N.D.
stress
LB Anaerobic with Chloramphenicol 0.000001953125 mM
N.D.
N.D.
-1.3
N.D.
N.D.
stress
2,4-Dinitrophenol 2 mM
N.D.
N.D.
-1.3
N.D.
N.D.
retron_ec_48_control
retron_Ec_48_control
N.D.
N.D.
-1.2
N.D.
N.D.
stress
LB Anaerobic with Furaltadone 0.000015625 mM
N.D.
N.D.
-1.2
N.D.
N.D.
stress
LB Anaerobic with Chloramphenicol 0.00000390625 mM
N.D.
N.D.
-1.2
N.D.
N.D.
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
N.D.
N.D.
-1.2
N.D.
N.D.
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
N.D.
N.D.
-1.2
N.D.
N.D.
mzaabcde
mzaABCDE
N.D.
N.D.
-1.1
N.D.
N.D.
mzaabcde
mzaABCDE
N.D.
N.D.
-1.1
N.D.
N.D.
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
N.D.
N.D.
-1.0
N.D.
N.D.
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
N.D.
N.D.
-1.0
N.D.
N.D.
carbon source
Ying_Nucleobase14 rep C; time point 1
N.D.
N.D.
+1.0
N.D.
N.D.
carbon source
Ying_Nucleobase14 rep A; time point 1
N.D.
N.D.
+1.0
N.D.
N.D.
carbon source
carbon source D-Glucose 0.25mM
N.D.
N.D.
+1.0
N.D.
N.D.
stress
Cholin acetate 75 mM
N.D.
N.D.
+1.1
N.D.
N.D.
stress
acetate 70 mM
N.D.
N.D.
+1.2
N.D.
N.D.
stress
Cholin acetate 150 mM
N.D.
N.D.
+1.4
N.D.
N.D.
stress
acetate 140 mM
N.D.
N.D.
+1.4
N.D.
N.D.
carbon source
Ying_Nucleobase14 rep C; time point 2
N.D.
N.D.
+1.7
N.D.
N.D.
carbon source
Ying_Nucleobase14 rep B; time point 2
N.D.
N.D.
+1.7
N.D.
N.D.
carbon source
Ying_Nucleobase14 rep A; time point 2
N.D.
N.D.
+1.8
N.D.
N.D.
carbon source
Ying_Nucleobase14 rep A; time point 3
N.D.
N.D.
+2.6
N.D.
N.D.
carbon source
Ying_Nucleobase14 rep C; time point 3
N.D.
N.D.
+2.6
N.D.
N.D.
carbon source
Ying_Nucleobase14 rep B; time point 3
N.D.
N.D.
+2.7
N.D.
N.D.
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