Fitness for 5 genes in Escherichia coli BW25113

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500 ntyjgL and argI are separated by 55 nucleotidesargI and yjgD are separated by 161 nucleotidesyjgD and yjgM are separated by 144 nucleotidesyjgM and yjgN are separated by 192 nucleotides b4253: yjgL - orf, hypothetical protein (VIMSS), at 4,473,460 to 4,475,274 yjgL b4254: argI - ornithine carbamoyltransferase 1 (NCBI), at 4,475,330 to 4,476,334 argI b4255: yjgD - hypothetical protein (NCBI), at 4,476,496 to 4,476,912 yjgD b4256: yjgM - predicted acetyltransferase (NCBI), at 4,477,057 to 4,477,560 yjgM b4257: yjgN - conserved inner membrane protein (NCBI), at 4,477,753 to 4,478,949 yjgN
Group Condition b4253 b4254 b4255 b4256 b4257
yjgL argI yjgD yjgM yjgN
carbon source Glycolic Acid (C) -1.3 +0.1 -0.3 -1.4 +0.0
carbon source Glycolic Acid (C) -1.0 +0.3 -0.9 -1.3 +0.1
M9 Glucose 2% aerobic -1.9 -0.4 -0.1 +0.1 -0.2
M9 Glucose 2% anaerobic -1.9 -0.4 -0.2 +0.1 -0.1
motility inner cut, LB soft agar motility assay -2.5 -0.1 -0.3 +0.0 +0.3
carbon source acetate (C) -0.8 -0.6 +0.2 -1.5 +0.3
motility outer cut, LB soft agar motility assay -1.3 +0.3 -1.0 +0.2 -0.4
stress Cisplatin 0.1 mg/ml -1.3 +0.3 -0.2 -0.2 -0.8
motility inner cut, LB soft agar motility assay -2.4 +0.2 +0.4 +0.0 -0.3
M9 Glucose 2%, 20 mM NO3- anaerobic -1.7 -0.4 -0.0 +0.2 -0.2
motility inner cut, LB soft agar motility assay -1.2 +0.2 -0.8 -0.0 -0.2
carbon source acetate (C) -0.7 -0.6 +0.3 -1.2 +0.2
M9 Acetate 0.5% aerobic -0.8 -0.3 +0.3 -0.9 -0.2
motility outer cut, LB soft agar motility assay -1.5 +0.3 -0.5 -0.0 +0.1
predation 6 hr rep2_HD100_10fold_dilution_grewON -0.4 +0.3 -2.0 +0.1 +0.4
stress Spectinomycin 0.0125 mg/ml -0.8 +0.3 -1.2 +0.2 +0.1
stress Benzalkonium Chloride 0.004 mg/ml -0.9 -0.1 +0.7 -0.1 -0.7
stress Cephalothin 0.01 mg/ml +0.7 +0.1 -1.5 +0.5 +0.4
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM +1.2 +0.1 -1.2 +0.0 +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.9 -0.1 +0.5 +0.0 -0.9
control PBS_cage_1_mouse_1_Cecum_40 hr +0.8 -0.2 -1.0 +0.2 +0.6
phage Δdbn_10 +0.8 -0.3 +0.7 -0.2 -0.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.2 +0.3 +1.0 +0.1 -1.0
carbon source carbon source D-Glucose 5mM +1.2 -0.4 -0.6 +0.3 +0.2
stress LB Anaerobic with Amoxicillin 0.0000078125 mM +0.9 +0.1 +0.6 +0.5 -0.8
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.7 -0.2 +1.2 +0.3 -0.3
carbon source Guanosine 0.35306 mM (C) +1.0 +0.0 +0.4 +0.8 -0.1
phage Papa dilution 10-1 cacl2 maltose +2.2 +0.1 -0.2 +0.1 +0.0
phage T7_phage 0.0015625 MOI TC2 +0.9 -0.1 +0.6 +0.3 +0.5
carbon source Ying_Nucleobase14 rep B; time point 3 +0.5 -0.0 +0.8 +1.0 +0.2
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