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Cofit
Protein
Homologs
Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yjgL and argI are separated by 55 nucleotides
argI and yjgD are separated by 161 nucleotides
yjgD and yjgM are separated by 144 nucleotides
yjgM and yjgN are separated by 192 nucleotides
b4253: yjgL - orf, hypothetical protein (VIMSS), at 4,473,460 to 4,475,274
yjgL
b4254: argI - ornithine carbamoyltransferase 1 (NCBI), at 4,475,330 to 4,476,334
argI
b4255: yjgD - hypothetical protein (NCBI), at 4,476,496 to 4,476,912
yjgD
b4256: yjgM - predicted acetyltransferase (NCBI), at 4,477,057 to 4,477,560
yjgM
b4257: yjgN - conserved inner membrane protein (NCBI), at 4,477,753 to 4,478,949
yjgN
Group
Condition
b4253
b4254
b4255
b4256
b4257
yjgL
argI
yjgD
yjgM
yjgN
carbon source
Glycolic Acid (C)
-1.3
+0.1
-0.3
-1.4
+0.0
carbon source
Glycolic Acid (C)
-1.0
+0.3
-0.9
-1.3
+0.1
M9 Glucose 2% aerobic
-1.9
-0.4
-0.1
+0.1
-0.2
M9 Glucose 2% anaerobic
-1.9
-0.4
-0.2
+0.1
-0.1
motility
inner cut, LB soft agar motility assay
-2.5
-0.1
-0.3
+0.0
+0.3
carbon source
acetate (C)
-0.8
-0.6
+0.2
-1.5
+0.3
motility
outer cut, LB soft agar motility assay
-1.3
+0.3
-1.0
+0.2
-0.4
stress
Cisplatin 0.1 mg/ml
-1.3
+0.3
-0.2
-0.2
-0.8
motility
inner cut, LB soft agar motility assay
-2.4
+0.2
+0.4
+0.0
-0.3
M9 Glucose 2%, 20 mM NO3- anaerobic
-1.7
-0.4
-0.0
+0.2
-0.2
motility
inner cut, LB soft agar motility assay
-1.2
+0.2
-0.8
-0.0
-0.2
carbon source
acetate (C)
-0.7
-0.6
+0.3
-1.2
+0.2
M9 Acetate 0.5% aerobic
-0.8
-0.3
+0.3
-0.9
-0.2
motility
outer cut, LB soft agar motility assay
-1.5
+0.3
-0.5
-0.0
+0.1
predation
6 hr rep2_HD100_10fold_dilution_grewON
-0.4
+0.3
-2.0
+0.1
+0.4
stress
Spectinomycin 0.0125 mg/ml
-0.8
+0.3
-1.2
+0.2
+0.1
stress
Benzalkonium Chloride 0.004 mg/ml
-0.9
-0.1
+0.7
-0.1
-0.7
stress
Cephalothin 0.01 mg/ml
+0.7
+0.1
-1.5
+0.5
+0.4
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
+1.2
+0.1
-1.2
+0.0
+0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.9
-0.1
+0.5
+0.0
-0.9
control
PBS_cage_1_mouse_1_Cecum_40 hr
+0.8
-0.2
-1.0
+0.2
+0.6
phage
Δdbn_10
+0.8
-0.3
+0.7
-0.2
-0.4
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.2
+0.3
+1.0
+0.1
-1.0
carbon source
carbon source D-Glucose 5mM
+1.2
-0.4
-0.6
+0.3
+0.2
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
+0.9
+0.1
+0.6
+0.5
-0.8
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.7
-0.2
+1.2
+0.3
-0.3
carbon source
Guanosine 0.35306 mM (C)
+1.0
+0.0
+0.4
+0.8
-0.1
phage
Papa dilution 10-1 cacl2 maltose
+2.2
+0.1
-0.2
+0.1
+0.0
phage
T7_phage 0.0015625 MOI TC2
+0.9
-0.1
+0.6
+0.3
+0.5
carbon source
Ying_Nucleobase14 rep B; time point 3
+0.5
-0.0
+0.8
+1.0
+0.2
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