Fitness for 5 genes in Escherichia coli BW25113

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500 ntnrdD and treC are separated by 393 nucleotidestreC and treB are separated by 49 nucleotidestreB and treR are separated by 118 nucleotidestreR and mgtL are separated by 184 nucleotides b4238: nrdD - anaerobic ribonucleoside triphosphate reductase (NCBI), at 4,458,545 to 4,460,683 nrdD b4239: treC - trehalose-6-P hydrolase (NCBI), at 4,461,077 to 4,462,732 treC b4240: treB - fused trehalose(maltose)-specific PTS enzyme: IIB component/IIC component (NCBI), at 4,462,782 to 4,464,203 treB b4241: treR - trehalose repressor (NCBI), at 4,464,322 to 4,465,269 treR b4702: mgtL - regulatory leader peptide for mgtA (NCBI), at 4,465,454 to 4,465,507 mgtL
Group Condition b4238 b4239 b4240 b4241 b4702
nrdD treC treB treR mgtL
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM -5.4 -0.9 -0.8 +1.0 -1.8
carbon source D-Trehalose (C) -0.1 -2.5 -2.4 +1.3 -3.2
carbon source D-Trehalose (C) -0.1 -2.3 -2.4 +1.3 -3.4
sulfur source M63 no sulfur + 5 mM L-cys -0.0 -0.4 -0.1 -0.3 -6.0
sulfur source M63 no sulfur + 5 mM L-cys -0.1 -0.7 +0.0 -0.3 -5.7
carbon source D-Xylose (C) +0.0 -1.6 -0.6 -0.0 -4.5
micoeukaryotes Dictyostellum sp.mixed culture-1 -0.0 -1.7 -0.4 +0.3 -4.9
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM -4.8 -0.8 -0.7 +0.8 -0.9
nitrogen source Putrescine (N) -0.1 -2.4 +0.2 +0.2 -4.3
stress LB Anaerobic with Oxytetracycline 0.00000015625 mM -4.1 -0.8 -0.6 +0.6 -1.3
stress LB + ciprofloxacin regrowth after 48hrs -5.6 -0.0 -0.4 +0.4 -0.6
stress LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM -4.7 -0.2 -0.4 +0.3 -1.1
lb LB regrowth after 48hrs -5.6 +0.1 -0.3 +0.3 -0.7
stress LB Anaerobic with Spiramycin 0.00003125 mM -3.8 -1.1 -0.4 +0.8 -1.4
stress LB + carbenicillin regrowth after 48hr -5.6 +0.1 -0.3 +0.4 -0.6
stress LB + carbenicillin regrowth after 48hr -5.6 +0.1 -0.3 +0.4 -0.5
lb LB regrowth after 48hrs -5.5 +0.4 -0.3 +0.3 -0.5
stress LB Anaerobic with Oxytetracycline 0.0000003125 mM -3.4 +0.1 -0.7 +0.7 -2.3
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.2 -1.3 -0.6 +0.7 -4.1
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -4.3 -0.6 +0.3 +0.4 -1.4
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -5.0 -0.6 +0.4 +0.6 -0.7
stress LB Anaerobic with Ramoplanin 0.0000625 mM -3.8 -0.9 -0.3 +0.7 -1.1
stress LB Anaerobic with Cephalothin 0.0000078125 mM -4.1 +0.3 -0.8 +0.5 -1.0
stress LB Anaerobic with Furaltadone 0.000015625 mM -3.5 +0.9 -1.3 +1.1 -2.1
stress LB Anaerobic with Spiramycin 0.000125 mM -2.8 -0.7 -0.3 +1.6 -2.0
carbon source Glycolic Acid (C) -0.0 +2.0 +0.0 -0.2 -4.7
phage Ste41 +0.6 +3.6 -0.6 +0.1 -2.2
phage Ste41 +0.7 +4.6 -0.2 +0.2 -1.4
stress Chlorite 1.25 mM -0.1 +3.7 +3.0 -0.4 +2.3
stress Phosphomycin 0.001 mg/ml -0.1 +10.2 -0.0 -0.1 -0.2
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