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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
nrdD and treC are separated by 393 nucleotides
treC and treB are separated by 49 nucleotides
treB and treR are separated by 118 nucleotides
treR and mgtL are separated by 184 nucleotides
b4238: nrdD - anaerobic ribonucleoside triphosphate reductase (NCBI), at 4,458,545 to 4,460,683
nrdD
b4239: treC - trehalose-6-P hydrolase (NCBI), at 4,461,077 to 4,462,732
treC
b4240: treB - fused trehalose(maltose)-specific PTS enzyme: IIB component/IIC component (NCBI), at 4,462,782 to 4,464,203
treB
b4241: treR - trehalose repressor (NCBI), at 4,464,322 to 4,465,269
treR
b4702: mgtL - regulatory leader peptide for mgtA (NCBI), at 4,465,454 to 4,465,507
mgtL
Group
Condition
b4238
b4239
b4240
b4241
b4702
nrdD
treC
treB
treR
mgtL
stress
LB Anaerobic with Phenazine ethosulfate 0.000125 mM
-5.4
-0.9
-0.8
+1.0
-1.8
carbon source
D-Trehalose (C)
-0.1
-2.5
-2.4
+1.3
-3.2
carbon source
D-Trehalose (C)
-0.1
-2.3
-2.4
+1.3
-3.4
sulfur source
M63 no sulfur + 5 mM L-cys
-0.0
-0.4
-0.1
-0.3
-6.0
sulfur source
M63 no sulfur + 5 mM L-cys
-0.1
-0.7
+0.0
-0.3
-5.7
carbon source
D-Xylose (C)
+0.0
-1.6
-0.6
-0.0
-4.5
micoeukaryotes
Dictyostellum sp.mixed culture-1
-0.0
-1.7
-0.4
+0.3
-4.9
stress
LB Anaerobic with Phenazine ethosulfate 0.000125 mM
-4.8
-0.8
-0.7
+0.8
-0.9
nitrogen source
Putrescine (N)
-0.1
-2.4
+0.2
+0.2
-4.3
stress
LB Anaerobic with Oxytetracycline 0.00000015625 mM
-4.1
-0.8
-0.6
+0.6
-1.3
stress
LB + ciprofloxacin regrowth after 48hrs
-5.6
-0.0
-0.4
+0.4
-0.6
stress
LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM
-4.7
-0.2
-0.4
+0.3
-1.1
lb
LB regrowth after 48hrs
-5.6
+0.1
-0.3
+0.3
-0.7
stress
LB Anaerobic with Spiramycin 0.00003125 mM
-3.8
-1.1
-0.4
+0.8
-1.4
stress
LB + carbenicillin regrowth after 48hr
-5.6
+0.1
-0.3
+0.4
-0.6
stress
LB + carbenicillin regrowth after 48hr
-5.6
+0.1
-0.3
+0.4
-0.5
lb
LB regrowth after 48hrs
-5.5
+0.4
-0.3
+0.3
-0.5
stress
LB Anaerobic with Oxytetracycline 0.0000003125 mM
-3.4
+0.1
-0.7
+0.7
-2.3
micoeukaryotes
Tetrahymena sp., Mixed culture-1
-0.2
-1.3
-0.6
+0.7
-4.1
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-4.3
-0.6
+0.3
+0.4
-1.4
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-5.0
-0.6
+0.4
+0.6
-0.7
stress
LB Anaerobic with Ramoplanin 0.0000625 mM
-3.8
-0.9
-0.3
+0.7
-1.1
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-4.1
+0.3
-0.8
+0.5
-1.0
stress
LB Anaerobic with Furaltadone 0.000015625 mM
-3.5
+0.9
-1.3
+1.1
-2.1
stress
LB Anaerobic with Spiramycin 0.000125 mM
-2.8
-0.7
-0.3
+1.6
-2.0
carbon source
Glycolic Acid (C)
-0.0
+2.0
+0.0
-0.2
-4.7
phage
Ste41
+0.6
+3.6
-0.6
+0.1
-2.2
phage
Ste41
+0.7
+4.6
-0.2
+0.2
-1.4
stress
Chlorite 1.25 mM
-0.1
+3.7
+3.0
-0.4
+2.3
stress
Phosphomycin 0.001 mg/ml
-0.1
+10.2
-0.0
-0.1
-0.2
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