Fitness for 5 genes in Escherichia coli BW25113

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500 ntnrdG and nrdD are separated by 157 nucleotidesnrdD and treC are separated by 393 nucleotidestreC and treB are separated by 49 nucleotidestreB and treR are separated by 118 nucleotides b4237: nrdG - anaerobic ribonucleotide reductase activating protein (NCBI), at 4,457,923 to 4,458,387 nrdG b4238: nrdD - anaerobic ribonucleoside triphosphate reductase (NCBI), at 4,458,545 to 4,460,683 nrdD b4239: treC - trehalose-6-P hydrolase (NCBI), at 4,461,077 to 4,462,732 treC b4240: treB - fused trehalose(maltose)-specific PTS enzyme: IIB component/IIC component (NCBI), at 4,462,782 to 4,464,203 treB b4241: treR - trehalose repressor (NCBI), at 4,464,322 to 4,465,269 treR
Group Condition b4237 b4238 b4239 b4240 b4241
nrdG nrdD treC treB treR
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM -5.7 -5.4 -0.9 -0.8 +1.0
stress LB + ciprofloxacin regrowth after 48hrs -6.0 -5.6 -0.0 -0.4 +0.4
lb LB Time48hr -6.0 -5.3 -0.1 -0.1 +0.1
lb LB regrowth after 48hrs -6.0 -5.6 +0.1 -0.3 +0.3
stress LB + carbenicillin regrowth after 48hr -6.0 -5.6 +0.1 -0.3 +0.4
stress LB + carbenicillin regrowth after 48hr -6.0 -5.6 +0.1 -0.3 +0.4
stress LB + carbenicillin Time48hr -5.9 -5.5 -0.0 -0.0 +0.2
stress LB + ciprofloxacin Time48hr -6.0 -5.4 +0.1 -0.2 +0.2
stress LB + carbenicillin Time8hr -5.7 -5.5 -0.0 -0.2 +0.2
lb LB regrowth after 48hrs -5.9 -5.5 +0.4 -0.3 +0.3
stress LB + ciprofloxacin Time24hr -5.6 -5.4 +0.0 -0.2 +0.2
stress LB + ciprofloxacin Time48hr -5.8 -5.3 +0.2 -0.1 +0.1
stress LB + carbenicillin Time6hr -5.7 -5.1 -0.1 -0.1 +0.1
stress LB + carbenicillin Time5hr -5.6 -5.2 -0.1 -0.1 +0.2
stress LB + ciprofloxacin Time9hr -5.6 -5.3 +0.2 -0.1 +0.1
stress LB + ciprofloxacin Time4hr -5.4 -5.2 -0.2 -0.2 +0.2
stress LB + ciprofloxacin Time24hr -5.6 -5.2 +0.1 -0.1 +0.2
stress LB + carbenicillin Time48hr -5.4 -5.4 +0.0 -0.1 +0.2
lb LB Time48hr -5.5 -5.2 +0.0 -0.1 +0.2
stress LB + carbenicillin Time5hr -5.5 -5.0 -0.1 -0.1 +0.2
stress LB + ciprofloxacin Time9hr -5.4 -5.3 +0.1 -0.1 +0.1
stress LB + carbenicillin Time6hr -5.8 -4.9 +0.1 -0.1 +0.2
stress LB + carbenicillin Time4hr -5.7 -4.9 +0.1 -0.1 +0.1
stress LB + carbenicillin Time9hr -5.8 -5.1 +0.4 -0.1 +0.2
lb LB Time5hr -5.5 -5.1 +0.1 -0.1 +0.2
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM -4.7 -4.8 -0.8 -0.7 +0.8
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -5.3 -5.0 -0.6 +0.4 +0.6
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM -4.7 -4.1 -0.4 -1.0 +0.9
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -5.0 -4.6 +0.3 -0.3 +0.7
stress LB Anaerobic with Furaltadone 0.000015625 mM -4.3 -3.5 +0.9 -1.3 +1.1
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