Fitness for 5 genes in Escherichia coli BW25113

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500 ntmpl and yjgA are separated by 155 nucleotidesyjgA and pmbA are separated by 93 nucleotidespmbA and cybC are separated by 178 nucleotidescybC and nrdG are separated by 44 nucleotides b4233: mpl - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase (NCBI), at 4,453,808 to 4,455,181 mpl b4234: yjgA - hypothetical protein (NCBI), at 4,455,337 to 4,455,888 yjgA b4235: pmbA - predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17 (NCBI), at 4,455,982 to 4,457,334 pmbA b4236: cybC - cytochrome b(562) (VIMSS), at 4,457,513 to 4,457,878 cybC b4237: nrdG - anaerobic ribonucleotide reductase activating protein (NCBI), at 4,457,923 to 4,458,387 nrdG
Group Condition b4233 b4234 b4235 b4236 b4237
mpl yjgA pmbA cybC nrdG
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.9 -1.1 -0.1 -0.3 -5.3
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.9 -0.7 -0.2 -0.5 -5.0
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.9 -1.7 -0.2 -0.3 -3.9
stress LB + carbenicillin regrowth after 48hr -0.3 -0.3 -0.0 -0.1 -6.0
stress LB + carbenicillin regrowth after 48hr -0.3 -0.3 -0.0 -0.1 -6.0
lb LB regrowth after 48hrs -0.0 -0.4 -0.1 -0.1 -5.9
stress LB + ciprofloxacin regrowth after 48hrs -0.1 -0.2 -0.1 -0.1 -6.0
lb LB regrowth after 48hrs -0.1 -0.1 -0.1 -0.0 -6.0
lb LB Time48hr -0.1 +0.2 +0.0 -0.1 -6.0
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM +0.3 -0.5 +0.1 -0.1 -5.7
stress LB + ciprofloxacin Time48hr -0.0 +0.1 -0.0 -0.1 -6.0
stress LB Anaerobic with Spiramycin 0.00003125 mM -0.2 -0.7 -0.1 -0.4 -4.6
stress LB + carbenicillin Time4hr +0.0 -0.1 -0.1 -0.0 -5.7
stress LB + carbenicillin Time6hr +0.0 +0.0 -0.1 -0.1 -5.8
stress LB + carbenicillin Time48hr -0.1 +0.1 -0.0 +0.0 -5.9
TN_15CS_15C_10dbl_rep3 -0.2 -4.8 -1.1 +0.1 +0.0
lb LB Time9hr -0.1 +0.2 -0.0 -0.2 -5.7
stress LB + ciprofloxacin Time48hr -0.0 +0.1 +0.0 -0.2 -5.8
stress LB + carbenicillin Time8hr +0.0 +0.0 -0.0 -0.1 -5.7
stress LB + carbenicillin Time9hr +0.0 +0.3 -0.0 -0.2 -5.8
stress LB + carbenicillin Time6hr +0.1 +0.1 -0.0 -0.1 -5.7
stress LB + ciprofloxacin Time9hr -0.1 +0.2 +0.0 -0.1 -5.6
stress LB Anaerobic with Cephalothin 0.0000078125 mM -2.0 +0.4 -0.5 +0.2 -3.7
stress LB Anaerobic with Furaltadone 0.000015625 mM -0.3 -0.4 -0.8 +0.3 -4.3
lb LB Time48hr -0.1 +0.3 -0.1 -0.1 -5.5
stress LB + ciprofloxacin Time24hr -0.1 +0.4 +0.0 -0.1 -5.6
stress LB + ciprofloxacin Time24hr -0.0 +0.2 -0.0 +0.0 -5.6
stress LB + ciprofloxacin Time7hr -0.0 +0.2 +0.0 -0.1 -5.4
stress LB + carbenicillin Time8hr +0.1 +0.3 -0.1 -0.1 -5.4
stress LB + carbenicillin Time7hr +0.0 +0.3 -0.1 +0.0 -5.4
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