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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
mpl and yjgA are separated by 155 nucleotides
yjgA and pmbA are separated by 93 nucleotides
pmbA and cybC are separated by 178 nucleotides
cybC and nrdG are separated by 44 nucleotides
b4233: mpl - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase (NCBI), at 4,453,808 to 4,455,181
mpl
b4234: yjgA - hypothetical protein (NCBI), at 4,455,337 to 4,455,888
yjgA
b4235: pmbA - predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17 (NCBI), at 4,455,982 to 4,457,334
pmbA
b4236: cybC - cytochrome b(562) (VIMSS), at 4,457,513 to 4,457,878
cybC
b4237: nrdG - anaerobic ribonucleotide reductase activating protein (NCBI), at 4,457,923 to 4,458,387
nrdG
Group
Condition
b4233
b4234
b4235
b4236
b4237
mpl
yjgA
pmbA
cybC
nrdG
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-0.9
-1.1
-0.1
-0.3
-5.3
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.9
-0.7
-0.2
-0.5
-5.0
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-0.9
-1.7
-0.2
-0.3
-3.9
stress
LB + carbenicillin regrowth after 48hr
-0.3
-0.3
-0.0
-0.1
-6.0
stress
LB + carbenicillin regrowth after 48hr
-0.3
-0.3
-0.0
-0.1
-6.0
lb
LB regrowth after 48hrs
-0.0
-0.4
-0.1
-0.1
-5.9
stress
LB + ciprofloxacin regrowth after 48hrs
-0.1
-0.2
-0.1
-0.1
-6.0
lb
LB regrowth after 48hrs
-0.1
-0.1
-0.1
-0.0
-6.0
lb
LB Time48hr
-0.1
+0.2
+0.0
-0.1
-6.0
stress
LB Anaerobic with Phenazine ethosulfate 0.000125 mM
+0.3
-0.5
+0.1
-0.1
-5.7
stress
LB + ciprofloxacin Time48hr
-0.0
+0.1
-0.0
-0.1
-6.0
stress
LB Anaerobic with Spiramycin 0.00003125 mM
-0.2
-0.7
-0.1
-0.4
-4.6
stress
LB + carbenicillin Time4hr
+0.0
-0.1
-0.1
-0.0
-5.7
stress
LB + carbenicillin Time6hr
+0.0
+0.0
-0.1
-0.1
-5.8
stress
LB + carbenicillin Time48hr
-0.1
+0.1
-0.0
+0.0
-5.9
TN_15CS_15C_10dbl_rep3
-0.2
-4.8
-1.1
+0.1
+0.0
lb
LB Time9hr
-0.1
+0.2
-0.0
-0.2
-5.7
stress
LB + ciprofloxacin Time48hr
-0.0
+0.1
+0.0
-0.2
-5.8
stress
LB + carbenicillin Time8hr
+0.0
+0.0
-0.0
-0.1
-5.7
stress
LB + carbenicillin Time9hr
+0.0
+0.3
-0.0
-0.2
-5.8
stress
LB + carbenicillin Time6hr
+0.1
+0.1
-0.0
-0.1
-5.7
stress
LB + ciprofloxacin Time9hr
-0.1
+0.2
+0.0
-0.1
-5.6
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-2.0
+0.4
-0.5
+0.2
-3.7
stress
LB Anaerobic with Furaltadone 0.000015625 mM
-0.3
-0.4
-0.8
+0.3
-4.3
lb
LB Time48hr
-0.1
+0.3
-0.1
-0.1
-5.5
stress
LB + ciprofloxacin Time24hr
-0.1
+0.4
+0.0
-0.1
-5.6
stress
LB + ciprofloxacin Time24hr
-0.0
+0.2
-0.0
+0.0
-5.6
stress
LB + ciprofloxacin Time7hr
-0.0
+0.2
+0.0
-0.1
-5.4
stress
LB + carbenicillin Time8hr
+0.1
+0.3
-0.1
-0.1
-5.4
stress
LB + carbenicillin Time7hr
+0.0
+0.3
-0.1
+0.0
-5.4
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