Fitness for 5 genes in Escherichia coli BW25113

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500 ntytfI and ytfJ are separated by 62 nucleotidesytfJ and ytfK are separated by 324 nucleotidesytfK and ytfL are separated by 78 nucleotidesytfL and msrA are separated by 322 nucleotides b4215: ytfI - hypothetical protein (NCBI), at 4,435,730 to 4,436,668 ytfI b4216: ytfJ - predicted transcriptional regulator (NCBI), at 4,436,731 to 4,437,285 ytfJ b4217: ytfK - orf, hypothetical protein (VIMSS), at 4,437,610 to 4,437,816 ytfK b4218: ytfL - predicted inner membrane protein (NCBI), at 4,437,895 to 4,439,238 ytfL b4219: msrA - methionine sulfoxide reductase A (NCBI), at 4,439,561 to 4,440,199 msrA
Group Condition b4215 b4216 b4217 b4218 b4219
ytfI ytfJ ytfK ytfL msrA
micoeukaryotes Dictyostellum sp.mixed culture-2 -0.7 +0.0 -0.4 -3.9 -0.1
micoeukaryotes Acanthamoeba sp., muxed culture-2 -0.0 +0.2 -0.6 -4.3 -0.2
stress LB Anaerobic Allura Red AC 250 uM -0.4 +0.0 -0.4 -3.3 -0.1
stress LB + carbenicillin regrowth after 48hr -0.2 -0.0 -0.1 -3.8 +0.1
stress LB Anaerobic Sulfasalazine 250 uM -0.1 +0.1 -0.8 -2.8 -0.3
micoeukaryotes Acanthamoeba sp., muxed culture-1 +1.1 -0.0 -0.8 -4.0 -0.0
stress LB Anaerobic Sulfasalazine 250 uM -0.1 +0.1 -0.0 -3.0 -0.6
micoeukaryotes Dictyostellum sp.mixed culture-1 +0.8 +0.0 -0.2 -3.8 -0.3
lb anaerobic no stress control LB anaerobic no stress control +0.3 +0.2 -0.5 -3.0 -0.3
stress LB + carbenicillin regrowth after 48hr +0.3 +0.0 +0.0 -3.5 -0.1
lb anaerobic no stress control LB anaerobic no stress control +0.2 +0.2 -0.3 -2.8 -0.6
stress LB Anaerobic Allura Red AC 250 uM +0.2 +0.1 -0.3 -2.9 -0.3
stress M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM -0.2 -0.0 -0.8 -2.2 +0.0
lb LB regrowth after 48hrs -0.1 -0.0 +0.1 -3.0 -0.0
m9 anaerobic no stress control M9 anaerobic no stress control +0.2 -0.2 -0.6 -2.3 -0.1
stress Bacitracin 1 mg/ml -1.1 +0.3 -0.6 -0.3 -1.3
carbon source carbon source D-Glucose 0.25mM -1.5 -0.1 -1.5 +0.1 +0.3
lb anaerobic no stress control LB anaerobic no stress control +0.6 +0.1 -0.4 -2.9 -0.1
predation 6 hr rep2_HD100_10fold_dilution_grewON -0.6 +0.4 -1.8 -0.3 -0.2
lb LB regrowth after 48hrs -0.4 +0.1 +0.2 -2.4 +0.1
control PBS_cage_2_mouse_1_Cecum_40 hr -2.4 -0.1 +0.2 -0.2 +0.3
carbon source carbon source D-Mannose 0.5mM -1.9 +0.5 -0.1 -0.1 -0.6
micoeukaryotes Tetrahymena sp., Mixed culture-1 +1.9 -0.2 -0.7 -2.6 -0.0
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.4 +0.1 -1.7 +0.7 -0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.5 +0.2 -1.4 +0.9 +0.2
stress M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM +1.4 +0.1 -0.3 -1.2 -0.4
phage Ste41 +1.5 +0.3 -0.8 -0.4 -1.0
control PBS_cage_1_mouse_2_Cecum_40 hr +1.8 -0.4 +0.1 -0.5 +0.2
control PBS_cage_1_mice _Feces_40 hr +2.0 -0.3 +0.3 -0.8 +0.5
nitrogen source Putrescine (N) -0.3 +0.0 +0.1 +4.1 +0.2
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