Fitness for 5 genes in Escherichia coli BW25113

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500 ntytfB and fklB are separated by 217 nucleotidesfklB and cycA are separated by 308 nucleotidescycA and ytfE are separated by 44 nucleotidesytfE and ytfF are separated by 107 nucleotides b4206: ytfB - orf, hypothetical protein (VIMSS), at 4,426,102 to 4,426,740 ytfB b4207: fklB - FKBP-type 22KD peptidyl-prolyl cis-trans isomerase (rotamase) (VIMSS), at 4,426,958 to 4,427,578 fklB b4208: cycA - D-alanine/D-serine/glycine transporter (NCBI), at 4,427,887 to 4,429,299 cycA b4209: ytfE - predicted regulator of cell morphogenesis and cell wall metabolism (NCBI), at 4,429,344 to 4,430,006 ytfE b4210: ytfF - predicted inner membrane protein (RefSeq), at 4,430,114 to 4,431,079 ytfF
Group Condition b4206 b4207 b4208 b4209 b4210
ytfB fklB cycA ytfE ytfF
carbon source Ying_all64 rep A; time point 3 +0.1 +0.0 -7.0 -0.3 -0.1
carbon source Ying_all64 rep C; time point 3 +0.0 +0.1 -6.9 -0.1 -0.1
carbon source Ying_all64 rep B; time point 3 -0.0 -0.0 -6.3 -0.2 +0.1
carbon source Ying_all64 rep A; time point 2 +0.2 +0.0 -4.5 -0.0 -0.1
carbon source Ying_all64 rep C; time point 2 +0.1 +0.1 -3.6 -0.2 +0.1
carbon source Ying_all64 rep B; time point 2 +0.0 +0.2 -3.2 -0.0 +0.0
carbon source D-Glucose (C) MOPS +0.0 +0.0 -2.8 -0.3 +0.1
carbon source D-Glucose (C) MOPS -0.2 +0.1 -2.7 -0.3 +0.1
stress LB Aerobic with Ramoplanin 0.000125 mM -1.7 +0.4 -1.3 -0.3 +0.0
stress LB Anaerobic with Cephalothin 0.0000078125 mM -2.0 +0.9 -0.8 -0.4 -0.2
carbon source D-Galacturonic Acid (C) MOPS +0.0 +0.2 -2.3 -0.4 -0.0
carbon source Ying_AminoAcid20 rep A; time point 3 +0.1 +0.0 -2.1 -0.2 -0.1
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.1 -0.2 -1.6 -0.1 -0.1
carbon source D-Galacturonic Acid (C) MOPS +0.0 +0.1 -2.1 -0.1 +0.0
stress LB Aerobic with Nitrofurantoin 0.00003125 mM +0.1 -0.1 -1.0 -1.1 +0.0
carbon source Ying_AminoAcid20 rep C; time point 3 -0.0 -0.0 -1.9 -0.1 +0.1
carbon source Ying_AminoAcid20 rep B; time point 3 +0.1 +0.1 -2.3 +0.1 +0.1
stress LB Aerobic with Ramoplanin 0.000125 mM -1.3 +0.4 -0.8 +0.0 -0.0
stress Carbenicillin 0.008 mg/ml -0.8 +0.4 -0.8 -0.3 -0.1
stress Cephalothin 0.01 mg/ml -1.3 +1.3 -1.4 +0.1 -0.2
stress LB Anaerobic with Cephalothin 0.0000078125 mM -1.6 +0.8 -0.7 +0.0 +0.2
stress Benzalkonium Chloride 0.004 mg/ml +0.4 +0.5 -2.2 +0.1 -0.0
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.9 +1.3 -0.1 -0.5 -0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.3 +0.2 +0.7 -0.9 +0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.8 +0.5 +0.7 +0.2 -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.3 +0.6 +1.0 -0.4 -0.3
stress 2-Furfuraldehyde 0.25 vol% -0.5 -0.8 +1.9 +0.3 -0.1
stress Cisplatin 0.1 mg/ml -0.5 +0.9 +0.2 +0.3 +0.6
stress Cisplatin 0.1 mg/ml -0.4 +1.2 +0.2 +0.0 +0.6
stress 3-methyl-1-butanol 1.25 vol% +0.2 +1.7 +1.1 +0.1 +0.1
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