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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
ulaC and ulaD are separated by 13 nucleotides
ulaD and ulaE are separated by 9 nucleotides
ulaE and ulaF overlap by 1 nucleotides
ulaF and yjfY are separated by 129 nucleotides
b4195: ulaC - L-ascorbate-specific enzyme IIA component of PTS (NCBI), at 4,419,731 to 4,420,195
ulaC
b4196: ulaD - 3-keto-L-gulonate 6-phosphate decarboxylase (NCBI), at 4,420,209 to 4,420,859
ulaD
b4197: ulaE - L-xylulose 5-phosphate 3-epimerase (NCBI), at 4,420,869 to 4,421,723
ulaE
b4198: ulaF - L-ribulose 5-phosphate 4-epimerase (NCBI), at 4,421,723 to 4,422,409
ulaF
b4199: yjfY - hypothetical protein (NCBI), at 4,422,539 to 4,422,814
yjfY
Group
Condition
b4195
b4196
b4197
b4198
b4199
ulaC
ulaD
ulaE
ulaF
yjfY
phage
RB68
-1.5
-0.4
-0.3
+0.0
-0.0
carbon source
carbon source D-Glucose 0.25mM
-0.4
-0.4
-0.4
-0.3
-0.3
stress
LB Anaerobic with Cefoxitin 0.000003125 mM
-0.9
-0.2
-0.5
-0.1
-0.1
stress
octanol 0.08 vol%
-1.4
+0.6
-0.3
-0.6
+0.3
phage
T7_phage 0.0015625 MOI TC2
+0.3
-0.8
-0.8
+0.2
-0.0
carbon source
carbon source D-Mannose 0.5mM
+0.0
+0.3
-0.4
-0.1
-0.8
predation
6 hr rep2_HD100_10fold_dilution_grewON
+0.2
+0.2
-0.3
-0.0
-0.9
stress
toluene 2.5 vol%
-0.7
-0.0
-0.4
+0.4
+0.1
carbon source
carbon source D-Mannose 5mM
+0.6
-1.0
-0.4
+0.2
+0.0
phage
T7_phage 0.00015625 MOI TC4
-0.2
-0.7
-0.1
+0.3
+0.2
nophagecontrol
R1_N4_T1_phage0
-0.6
+0.6
+0.2
-0.4
-0.2
stress
LB Aerobic with Hygromycin B 0.000015625 mM
+0.6
-0.5
-0.4
+0.1
-0.1
stress
Chlorite 1.25 mM
+0.7
-0.5
+0.4
-0.3
-0.4
carbon source
carbon source D-Glucose 0.25mM
-0.2
+0.5
-0.4
+0.3
-0.1
control
PBS_cage_1_mice _Feces_16 hr
+0.4
+0.3
+0.3
-0.3
-0.6
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-0.8
+0.2
-0.0
+0.5
+0.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.2
+0.9
-0.3
-0.2
-0.1
msc
22hr_MSC_rep1_highinoculum
+0.9
-0.6
-0.0
-0.1
+0.1
motility
outer cut, LB soft agar motility assay
-0.5
-0.1
-0.2
+0.6
+0.4
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
-0.4
+0.1
+0.7
+0.2
-0.2
stress
LB Aerobic with Zinc Pyrithione 0.00000125 mM
+0.8
-0.5
-0.1
+0.3
-0.0
phage
Δdbn_10
+0.9
+0.3
-0.6
+0.2
-0.0
phage
Ste41
-0.0
+0.1
-0.5
+0.6
+0.5
control
PBS_cage_1_mouse_1_Cecum_40 hr
+0.2
+0.7
+0.5
-0.1
-0.3
motility
inner cut, LB soft agar motility assay
+0.7
+0.4
-0.1
+0.2
+0.2
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.8
+0.3
+0.3
+0.3
-0.2
stress
Chloramphenicol 0.001 mg/ml
+1.0
+0.3
+0.3
+0.1
-0.1
stress
Cisplatin 0.1 mg/ml
+0.2
+1.0
-0.1
+0.3
+0.1
motility
inner cut, LB soft agar motility assay
+0.6
+0.6
+0.3
+0.1
-0.0
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
+1.1
+0.2
+0.2
+0.2
+0.4
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