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Cofit
Protein
Homologs
Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yjfJ and yjfK are separated by 50 nucleotides
yjfK and yjfL are separated by 17 nucleotides
yjfL and yjfM are separated by 9 nucleotides
yjfM and yjfC are separated by 2 nucleotides
b4182: yjfJ - predicted transcriptional regulator effector protein (NCBI), at 4,408,576 to 4,409,274
yjfJ
b4183: yjfK - hypothetical protein (NCBI), at 4,409,325 to 4,409,984
yjfK
b4184: yjfL - conserved inner membrane protein (NCBI), at 4,410,002 to 4,410,400
yjfL
b4185: yjfM - hypothetical protein (NCBI), at 4,410,410 to 4,411,048
yjfM
b4186: yjfC - predicted synthetase/amidase (NCBI), at 4,411,051 to 4,412,214
yjfC
Group
Condition
b4182
b4183
b4184
b4185
b4186
yjfJ
yjfK
yjfL
yjfM
yjfC
control
PBS_cage_2_mouse_2_Cecum_40 hr
+0.3
-0.4
-2.5
+0.0
-0.4
carbon source
carbon source D-Mannose 5mM
-0.4
+0.4
-1.5
-0.5
-0.4
carbon source
carbon source D-Mannose 0.5mM
+0.0
+0.6
-2.1
-0.4
-0.4
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.3
-0.3
-1.2
-0.4
+0.1
phage
Ste41
-0.1
-0.5
-0.5
-0.8
-0.2
M9 Acetate 0.5% aerobic
-0.2
-0.2
-1.5
+0.2
-0.2
control
PBS_cage_1_mouse_2_Cecum_40 hr
-0.3
+0.0
-1.1
-0.0
-0.5
predation
3 hr rep1_HD100_no_dilution
-0.2
-0.0
-1.3
+0.1
-0.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.6
-0.4
-0.2
-0.3
-0.1
carbon source
carbon source D-Glucose 0.25mM
-0.0
+0.0
-1.8
-0.0
+0.3
carbon source
carbon source D-Glucose 5mM
-0.3
-0.2
-1.0
+0.5
-0.3
stress
Benzalkonium Chloride 0.004 mg/ml
-0.0
+0.3
-0.7
+0.0
-0.9
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.2
-0.3
-0.9
-0.3
+0.5
M9 Acetate 0.5% aerobic
-0.1
-0.2
-1.1
+0.3
-0.1
control
PBS_cage_2_mouse_1_Cecum_40 hr
+0.2
-0.1
-1.1
-0.0
-0.1
stress
LB Anaerobic with Ramoplanin 0.0000625 mM
-0.1
-0.2
-0.9
-0.1
+0.2
stress
Ciprofloxacin Hydrochloride 0.0003 mM
+0.0
+0.1
-1.3
+0.2
-0.1
nopredatorcontrol
3 hr NoPredatorControl
+0.0
+0.2
-1.1
-0.1
+0.1
phage
RB68
+0.2
-0.0
-0.5
-0.6
+0.3
carbon source
carbon source D-Mannose 1mM
+0.4
+0.2
-0.9
-0.1
-0.1
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.1
-0.4
+0.7
+0.1
-0.4
mzaabcde
mzaABCDE
+0.1
+0.3
-0.8
+0.0
+0.3
phage
TP4
-0.0
+0.4
-0.7
+0.4
+0.3
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
+0.4
+0.5
-0.3
-0.4
+0.2
motility
inner cut, LB soft agar motility assay
+0.3
+0.0
-0.5
+0.4
+0.4
phage
T7_phage 0.0015625 MOI TC2
-0.2
+0.1
+1.2
-0.2
+0.0
stress
LB Aerobic with Irgasan 0.0000003125 mM
+0.2
-0.1
+1.0
+0.0
+0.3
phage
T7_phage 0.0015625 MOI TC1
+0.2
+0.2
+0.9
-0.1
+0.3
phage
Papa dilution 10-1 cacl2 maltose
+0.3
+0.4
+0.3
+0.4
+0.3
phage
T7_phage 0.00015625 MOI TC4
+0.4
+0.2
+0.7
+0.0
+0.6
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