Fitness for 5 genes in Escherichia coli BW25113

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500 ntyjfJ and yjfK are separated by 50 nucleotidesyjfK and yjfL are separated by 17 nucleotidesyjfL and yjfM are separated by 9 nucleotidesyjfM and yjfC are separated by 2 nucleotides b4182: yjfJ - predicted transcriptional regulator effector protein (NCBI), at 4,408,576 to 4,409,274 yjfJ b4183: yjfK - hypothetical protein (NCBI), at 4,409,325 to 4,409,984 yjfK b4184: yjfL - conserved inner membrane protein (NCBI), at 4,410,002 to 4,410,400 yjfL b4185: yjfM - hypothetical protein (NCBI), at 4,410,410 to 4,411,048 yjfM b4186: yjfC - predicted synthetase/amidase (NCBI), at 4,411,051 to 4,412,214 yjfC
Group Condition b4182 b4183 b4184 b4185 b4186
yjfJ yjfK yjfL yjfM yjfC
control PBS_cage_2_mouse_2_Cecum_40 hr +0.3 -0.4 -2.5 +0.0 -0.4
carbon source carbon source D-Mannose 5mM -0.4 +0.4 -1.5 -0.5 -0.4
carbon source carbon source D-Mannose 0.5mM +0.0 +0.6 -2.1 -0.4 -0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.3 -0.3 -1.2 -0.4 +0.1
phage Ste41 -0.1 -0.5 -0.5 -0.8 -0.2
M9 Acetate 0.5% aerobic -0.2 -0.2 -1.5 +0.2 -0.2
control PBS_cage_1_mouse_2_Cecum_40 hr -0.3 +0.0 -1.1 -0.0 -0.5
predation 3 hr rep1_HD100_no_dilution -0.2 -0.0 -1.3 +0.1 -0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.6 -0.4 -0.2 -0.3 -0.1
carbon source carbon source D-Glucose 0.25mM -0.0 +0.0 -1.8 -0.0 +0.3
carbon source carbon source D-Glucose 5mM -0.3 -0.2 -1.0 +0.5 -0.3
stress Benzalkonium Chloride 0.004 mg/ml -0.0 +0.3 -0.7 +0.0 -0.9
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.2 -0.3 -0.9 -0.3 +0.5
M9 Acetate 0.5% aerobic -0.1 -0.2 -1.1 +0.3 -0.1
control PBS_cage_2_mouse_1_Cecum_40 hr +0.2 -0.1 -1.1 -0.0 -0.1
stress LB Anaerobic with Ramoplanin 0.0000625 mM -0.1 -0.2 -0.9 -0.1 +0.2
stress Ciprofloxacin Hydrochloride 0.0003 mM +0.0 +0.1 -1.3 +0.2 -0.1
nopredatorcontrol 3 hr NoPredatorControl +0.0 +0.2 -1.1 -0.1 +0.1
phage RB68 +0.2 -0.0 -0.5 -0.6 +0.3
carbon source carbon source D-Mannose 1mM +0.4 +0.2 -0.9 -0.1 -0.1
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.1 -0.4 +0.7 +0.1 -0.4
mzaabcde mzaABCDE +0.1 +0.3 -0.8 +0.0 +0.3
phage TP4 -0.0 +0.4 -0.7 +0.4 +0.3
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.4 +0.5 -0.3 -0.4 +0.2
motility inner cut, LB soft agar motility assay +0.3 +0.0 -0.5 +0.4 +0.4
phage T7_phage 0.0015625 MOI TC2 -0.2 +0.1 +1.2 -0.2 +0.0
stress LB Aerobic with Irgasan 0.0000003125 mM +0.2 -0.1 +1.0 +0.0 +0.3
phage T7_phage 0.0015625 MOI TC1 +0.2 +0.2 +0.9 -0.1 +0.3
phage Papa dilution 10-1 cacl2 maltose +0.3 +0.4 +0.3 +0.4 +0.3
phage T7_phage 0.00015625 MOI TC4 +0.4 +0.2 +0.7 +0.0 +0.6
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