Fitness for 5 genes in Escherichia coli BW25113

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500 ntyjeO and yjeP are separated by 28 nucleotidesyjeP and psd are separated by 21 nucleotidespsd and yjeQ are separated by 96 nucleotidesyjeQ and yjeR are separated by 94 nucleotides b4158: yjeO - conserved inner membrane protein (NCBI), at 4,383,727 to 4,384,041 yjeO b4159: yjeP - predicted mechanosensitive channel (NCBI), at 4,384,070 to 4,387,393 yjeP b4160: psd - phosphatidylserine decarboxylase (NCBI), at 4,387,415 to 4,388,383 psd b4161: yjeQ - orf, hypothetical protein (VIMSS), at 4,388,480 to 4,389,532 yjeQ b4162: yjeR - orf, hypothetical protein (VIMSS), at 4,389,627 to 4,390,172 yjeR
Group Condition b4158 b4159 b4160 b4161 b4162
yjeO yjeP psd yjeQ yjeR
stress Benzalkonium Chloride 0.004 mg/ml -1.6 -3.7 N.D. N.D. N.D.
stress LB Aerobic with Benzethonium chloride 0.000015625 mM -0.1 -5.1 N.D. N.D. N.D.
stress LB Aerobic with Benzethonium chloride 0.000015625 mM +0.2 -4.9 N.D. N.D. N.D.
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.2 -3.2 N.D. N.D. N.D.
stress LB Aerobic with Novobiocin 0.0000625 mM -0.4 -2.2 N.D. N.D. N.D.
stress octanol 0.08 vol% -1.8 -0.3 N.D. N.D. N.D.
stress LB Aerobic with Novobiocin 0.0000625 mM -0.0 -2.1 N.D. N.D. N.D.
stress Spectinomycin 0.0125 mg/ml -0.3 -1.6 N.D. N.D. N.D.
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.8 -2.7 N.D. N.D. N.D.
phage Ste41 -1.5 -0.2 N.D. N.D. N.D.
phage Ste41 -0.9 -0.5 N.D. N.D. N.D.
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.4 -0.9 N.D. N.D. N.D.
carbon source carbon source D-Mannose 1mM -1.1 -0.1 N.D. N.D. N.D.
stress LB Aerobic with Irgasan 0.0000003125 mM -0.2 -0.9 N.D. N.D. N.D.
stress LB Aerobic with Spiramycin 0.000125 mM -0.5 -0.7 N.D. N.D. N.D.
stress LB Anaerobic with Sulfamonomethoxine 0.0000625 mM -1.0 -0.1 N.D. N.D. N.D.
carbon source carbon source D-Glucose 20mM -1.1 +0.0 N.D. N.D. N.D.
stress Spectinomycin 0.00625 mg/ml -0.1 -0.9 N.D. N.D. N.D.
control PBS_cage_2_mice _Feces_16 hr -0.9 -0.1 N.D. N.D. N.D.
stress LB Aerobic with Spiramycin 0.0000625 mM -0.6 -0.4 N.D. N.D. N.D.
carbon source carbon source D-Mannose 5mM -1.1 +0.2 N.D. N.D. N.D.
vitamin M9 with Pyridoxine HCl 20 nM and 4-Deoxypyridoxine 0.25 uM -0.9 +0.1 N.D. N.D. N.D.
stress Cisplatin 0.1 mg/ml -0.9 +0.3 N.D. N.D. N.D.
carbon source Acetate (C) -0.7 +0.4 N.D. N.D. N.D.
stress LB Aerobic with Spiramycin 0.000125 mM +0.6 -0.7 N.D. N.D. N.D.
stress LB Anaerobic with Norfloxacin 0.00000015625 mM +1.0 -0.0 N.D. N.D. N.D.
stress Cobalt chloride 0.32 mM +0.9 +0.1 N.D. N.D. N.D.
stress Cisplatin 0.05 mg/ml +0.9 +0.1 N.D. N.D. N.D.
lb LB +0.9 +0.1 N.D. N.D. N.D.
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +1.1 +0.2 N.D. N.D. N.D.
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