Fitness for 5 genes in Escherichia coli BW25113

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500 ntyjeJ and yjeK are separated by 394 nucleotidesyjeK and efp are separated by 41 nucleotidesefp and ecnA are separated by 51 nucleotidesecnA and ecnB are separated by 110 nucleotides b4145: yjeJ - hypothetical protein (NCBI), at 4,371,388 to 4,372,257 yjeJ b4146: yjeK - predicted lysine aminomutase (NCBI), at 4,372,652 to 4,373,680 yjeK b4147: efp - elongation factor P (NCBI), at 4,373,722 to 4,374,288 efp b4410: ecnA - entericidin A membrane lipoprotein, antidote entericidin B (NCBI), at 4,374,340 to 4,374,465 ecnA b4411: ecnB - entericidin B membrane lipoprotein (NCBI), at 4,374,576 to 4,374,722 ecnB
Group Condition b4145 b4146 b4147 b4410 b4411
yjeJ yjeK efp ecnA ecnB
msc 22hr_MSC_rep1_highinoculum +0.4 -1.7 N.D. -6.2 -0.3
carbon source carbon source D-Mannose 0.5mM -0.2 -3.1 N.D. -0.4 -2.5
control PBS_cage_1_mouse_1_Cecum_40 hr -0.5 -2.7 N.D. -1.4 -1.5
carbon source carbon source D-Mannose 1mM -0.1 -2.1 N.D. -1.7 -1.8
phage T7_phage 0.0015625 MOI TC2 +0.5 -0.9 N.D. -4.8 +0.2
carbon source carbon source D-Mannose 5mM -0.2 -1.9 N.D. -3.5 +1.0
phage Phage_N4_cage_4_mouse_2_Cecum_40 hr +0.1 -2.4 N.D. -1.4 -0.9
control PBS_cage_1_mice _Feces_22 hr -0.2 -2.5 N.D. -1.9 +0.1
control PBS_cage_2_mice _Feces_22 hr -0.0 -3.5 N.D. -1.2 +0.1
stress 2,4-Dinitrophenol 2 mM +0.1 -3.5 N.D. -0.6 -0.1
control PBS_cage_1_mice _Feces_40 hr -0.1 -2.9 N.D. -1.3 +0.3
nopredatorcontrol 3 hr NoPredatorControl -0.3 -2.2 N.D. -1.6 +0.2
control NoPredeControl -0.0 -2.0 N.D. -1.4 -0.5
stress Benzalkonium Chloride 0.004 mg/ml +0.1 -1.2 N.D. -3.1 +0.4
stress Aluminum chloride 5 mM +0.2 -1.8 N.D. -0.6 -1.7
predation 18 hr rep1_HD100_10fold_dilution -0.2 -2.6 N.D. -1.1 +0.1
phage Δdbn_10 -0.3 +0.7 N.D. -3.3 -0.7
control PBS_cage_2_mouse_3_Cecum_40 hr +0.2 -1.6 N.D. -1.9 -0.3
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.6 +0.1 N.D. -3.7 -0.7
micoeukaryotes Acanthamoeba sp., muxed culture-1 +0.1 -2.8 N.D. -0.8 +0.4
stress octanol 0.08 vol% -0.5 -4.1 N.D. +1.1 +0.7
carbon source carbon source D-Glucose 20mM -0.0 -2.4 N.D. +0.8 -1.2
stress undecene 5 vol% -0.1 -2.5 N.D. +0.7 -0.7
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr -0.3 -2.5 N.D. +1.0 -0.6
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.7 -2.3 N.D. +0.9 +0.1
phage T7_phage 0.0015625 MOI TC1 -0.2 -2.3 N.D. +0.3 +1.2
stress 3-methyl-1-butanol 1.25 vol% +0.2 -2.3 N.D. +0.8 +1.0
motility outer cut, LB soft agar motility assay -0.7 +1.3 N.D. -1.5 +0.7
predation 3 hr rep1_HD100_100fold_dilution -0.3 -1.9 N.D. +1.6 +0.3
carbon source a-Ketoglutaric (C) +0.1 +2.0 N.D. -1.3 -1.0
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