Fitness for 5 genes in Escherichia coli BW25113

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500 ntaspA and b4140 are separated by 336 nucleotidesb4140 and yjeH are separated by 15 nucleotidesyjeH and groS are separated by 275 nucleotidesgroS and GroEL are separated by 43 nucleotides b4139: aspA - aspartate ammonia-lyase (aspartase) (VIMSS), at 4,364,914 to 4,366,350 aspA b4140: b4140 - orf, hypothetical protein (VIMSS), at 4,366,687 to 4,367,163 b4140 b4141: yjeH - predicted transporter (NCBI), at 4,367,179 to 4,368,435 yjeH b4142: groS - co-chaperonin GroES (NCBI), at 4,368,711 to 4,369,004 groS b4143: GroEL - chaperonin GroEL (NCBI), at 4,369,048 to 4,370,694 GroEL
Group Condition b4139 b4140 b4141 b4142 b4143
aspA b4140 yjeH groS GroEL
carbon source Ying_AminoAcid20 rep C; time point 3 -6.3 -0.1 +0.1 N.D. N.D.
carbon source Ying_AminoAcid20 rep B; time point 3 -5.8 -0.2 +0.0 N.D. N.D.
carbon source Ying_AminoAcid20 rep A; time point 3 -5.6 +0.0 +0.0 N.D. N.D.
carbon source Ying_AminoAcid20 rep C; time point 2 -4.9 -0.1 -0.0 N.D. N.D.
carbon source Ying_AminoAcid20 rep A; time point 2 -4.6 -0.2 -0.1 N.D. N.D.
carbon source Ying_AminoAcid20 rep B; time point 2 -4.7 -0.1 +0.1 N.D. N.D.
carbon source casaminos (C) -3.8 -0.1 -0.0 N.D. N.D.
carbon source casaminos (C) -3.8 -0.1 +0.2 N.D. N.D.
micoeukaryotes Tetrahymena sp., Mixed culture-1 -3.4 -0.2 -0.0 N.D. N.D.
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -2.6 -0.1 -0.2 N.D. N.D.
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.4 -2.3 -0.1 N.D. N.D.
fishing LB+Gent outgrowth of mutant library after 1 day conjugation with donor carrying pRH22 VcDART vector -2.8 -0.1 +0.1 N.D. N.D.
fishing LB+Gent outgrowth of mutant library after 1 day conjugation with donor carrying pRH23 VcDART vector -2.6 -0.0 -0.0 N.D. N.D.
nitrogen source Gly-DL-Asp (N) -2.7 -0.1 +0.1 N.D. N.D.
mzaabcde mzaABCDE -2.4 -0.2 -0.0 N.D. N.D.
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -2.4 -0.2 -0.0 N.D. N.D.
nitrogen source Gly-DL-Asp (N) -2.5 -0.1 +0.1 N.D. N.D.
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.4 -2.2 +0.1 N.D. N.D.
stress LB Anaerobic with Cephalothin 0.0000078125 mM -1.6 -0.9 +0.1 N.D. N.D.
gfp_control GFP_control -1.7 -0.6 -0.2 N.D. N.D.
drt_type_3 DRT_type_3 -2.1 -0.2 -0.2 N.D. N.D.
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.5 -1.9 +0.0 N.D. N.D.
mixed community LB 0.1x with Mixed culture; Trichoderma virens G41 -2.1 -0.3 +0.1 N.D. N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay +1.9 -1.7 -0.1 N.D. N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay +2.1 -1.7 -0.3 N.D. N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay +1.9 -1.2 -0.1 N.D. N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay +4.8 -2.8 -0.4 N.D. N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay +3.6 -1.7 -0.1 N.D. N.D.
phage T7_phage 0.00015625 MOI TC4 +3.4 +0.0 -0.4 N.D. N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay +4.3 -1.1 -0.1 N.D. N.D.
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