Fitness for 5 genes in Escherichia coli BW25113

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500 ntyjdQ and pheU are separated by 197 nucleotidespheU and yjdC are separated by 106 nucleotidesyjdC and dipZ are separated by 36 nucleotidesdipZ and cutA overlap by 25 nucleotides b4654: yjdQ - no description, at 4,360,214 to 4,360,376 yjdQ b4134: pheU - tRNA-Phe (NCBI), at 4,360,574 to 4,360,649 pheU b4135: yjdC - orf, hypothetical protein (VIMSS), at 4,360,756 to 4,361,331 yjdC b4136: dipZ - thiol:disulfide interchange protein precursor (NCBI), at 4,361,368 to 4,363,065 dipZ b4137: cutA - copper binding protein, copper sensitivity (NCBI), at 4,363,041 to 4,363,379 cutA
Group Condition b4654 b4134 b4135 b4136 b4137
yjdQ pheU yjdC dipZ cutA
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.1 N.D. -0.4 -0.8 -0.9
stress Cisplatin 0.1 mg/ml -0.0 N.D. -0.6 -0.1 -1.2
gfp_control GFP_control -0.8 N.D. -0.4 -0.5 +0.1
stress BHIS with Methotrexate 1.98 mM 24 hr -1.3 N.D. +0.0 -0.2 -0.2
no stress control BHIS no stress control; Dimethyl Sulfoxide 1 vol% 24 hr -1.2 N.D. +0.1 -0.2 -0.2
stress BHIS with Methotrexate 0.06 mM 48 hr -1.3 N.D. +0.1 -0.1 -0.3
no stress control BHIS no stress control; Dimethyl Sulfoxide 1 vol% 48 hr -1.3 N.D. +0.2 +0.0 -0.3
stress BHIS with Methotrexate 0.1 mM 48 hr -1.2 N.D. +0.1 -0.0 -0.2
stress Bacitracin 1 mg/ml +0.3 N.D. +0.1 -0.8 -0.5
carbon source carbon source D-Mannose 1mM -0.3 N.D. +0.6 -0.5 -0.7
phage RB68 -1.0 N.D. -0.2 +0.3 +0.3
motility outer cut, LB soft agar motility assay -0.3 N.D. -0.8 +0.1 +0.5
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.7 N.D. -0.5 -0.2 -0.3
phage T7_phage 0.0015625 MOI TC2 +1.0 N.D. +0.2 -0.4 -0.9
carbon source carbon source D-Mannose 0.5mM +0.1 N.D. +0.6 -1.0 +0.1
stress LB Aerobic with Irgasan 0.0000003125 mM -0.2 N.D. +0.6 +0.3 -0.7
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x +0.2 N.D. +0.7 -0.4 -0.3
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x -0.1 N.D. +1.0 -0.5 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +1.1 N.D. -0.2 -0.4 +0.0
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x +0.0 N.D. +1.1 -0.6 -0.1
carbon source Ying_Nucleobase14 rep A; time point 2 +0.3 N.D. +0.9 -0.3 -0.2
carbon source Ying_Nucleobase14 rep C; time point 3 +0.3 N.D. +1.0 -0.4 -0.1
carbon source Ying_Nucleobase14 rep A; time point 3 +0.3 N.D. +1.1 -0.5 -0.1
carbon source Ying_sugar7 rep C; time point 3 +0.3 N.D. +0.9 -0.5 +0.1
stress limonene 5 vol% +1.3 N.D. -0.2 -0.2 +0.1
carbon source Ying_Nucleobase14 rep B; time point 3 +0.3 N.D. +1.1 -0.3 -0.1
carbon source Ying_sugar7 rep B; time point 3 +0.3 N.D. +0.8 -0.4 +0.3
carbon source Ying_sugar7 rep A; time point 3 +0.3 N.D. +0.7 -0.3 +0.4
control PBS_cage_1_mice _Feces_16 hr +0.0 N.D. +0.9 -0.3 +0.5
stress limonene 5 vol% +1.3 N.D. -0.3 -0.1 +0.3
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