Fitness for 5 genes in Escherichia coli BW25113

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500 ntfumB and dcuB are separated by 77 nucleotidesdcuB and dcuR are separated by 570 nucleotidesdcuR and dcuS overlap by 4 nucleotidesdcuS and yjdI are separated by 180 nucleotides b4122: fumB - anaerobic class I fumarate hydratase (fumarase B) (NCBI), at 4,343,703 to 4,345,349 fumB b4123: dcuB - C4-dicarboxylate antiporter (NCBI), at 4,345,427 to 4,346,767 dcuB b4124: dcuR - DNA-binding response regulator in two-component regulatory system with DcuS (NCBI), at 4,347,338 to 4,348,057 dcuR b4125: dcuS - sensory histidine kinase in two-component regulatory system with DcuR, regulator of anaerobic fumarate respiration (NCBI), at 4,348,054 to 4,349,685 dcuS b4126: yjdI - hypothetical protein (NCBI), at 4,349,866 to 4,350,096 yjdI
Group Condition b4122 b4123 b4124 b4125 b4126
fumB dcuB dcuR dcuS yjdI
carbon source L-Malic (C) -0.1 -0.2 -4.2 -4.4 +0.2
carbon source L-Malic (C) +0.0 -0.2 -4.1 -4.1 +0.2
carbon source succinate (C) -0.0 -0.1 -4.0 -4.0 +0.5
carbon source succinate (C) -0.2 -0.3 -3.8 -3.9 +0.6
carbon source Ying_OrganicAcid7 rep C; time point 3 -0.0 -0.0 -1.6 -1.6 +0.2
carbon source Ying_OrganicAcid7 rep A; time point 3 -0.0 -0.0 -1.4 -1.7 +0.3
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.1 -0.1 -1.3 -1.3 -0.2
carbon source Ying_OrganicAcid7 rep B; time point 3 -0.1 -0.0 -1.1 -1.3 +0.3
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.1 +0.2 -1.3 -1.2 +0.1
carbon source Ying_OrganicAcid7 rep B; time point 2 -0.2 -0.1 -0.8 -0.9 +0.2
carbon source Ying_OrganicAcid7 rep A; time point 2 -0.2 +0.0 -0.9 -0.9 +0.2
stress LB Anaerobic with Ticarcillin 0.00003125 mM -0.1 -0.0 -0.9 -0.9 +0.3
carbon source Ying_OrganicAcid7 rep C; time point 2 -0.1 -0.0 -0.8 -0.9 +0.3
stress LB Anaerobic with Amoxicillin 0.0000078125 mM +0.3 -0.1 -0.8 -0.9 +0.1
carbon source a-Ketoglutaric (C) -0.0 -0.0 -0.8 -0.8 +0.4
carbon source casaminos (C) +0.1 -0.1 -0.7 -0.6 +0.2
stress LB Anaerobic with Amoxicillin 0.0000078125 mM +0.1 +0.1 -0.8 -0.8 +0.4
phage Ste41 +0.5 -0.1 -0.3 -0.5 -0.5
carbon source a-Ketoglutaric (C) -0.0 -0.1 -0.7 -0.6 +0.5
phage RB68 +0.7 -0.0 +0.2 -0.8 -0.4
phage Ste41 +0.9 +0.4 +0.0 -0.6 -0.7
no stress control anaerobic M9 no stress control; Anaerobic 24 hr +0.7 +0.1 +0.7 +0.5 -0.5
no stress control anaerobic M9 no stress control; Anaerobic 48 hr +0.8 +0.1 +0.7 +0.6 -0.5
no stress control anaerobic M9 no stress control; Anaerobic 24 hr +0.8 +0.0 +0.7 +0.6 -0.3
no stress control anaerobic M9 no stress control; Anaerobic 48 hr +0.8 -0.0 +0.7 +0.6 -0.3
no stress control anaerobic M9 no stress control; Anaerobic 24 hr +0.8 +0.0 +0.8 +0.7 -0.4
carbon source D-Galacturonic Acid (C) MOPS -0.0 +0.3 +1.0 +0.8 -0.3
phage T7_phage 0.00015625 MOI TC4 +0.2 +0.5 +0.4 +0.6 +0.3
no stress control anaerobic M9 no stress control; Anaerobic 48 hr +0.8 +0.1 +0.8 +0.7 -0.4
stress LB Aerobic with Nitrofurantoin 0.00003125 mM +0.1 +0.1 +0.9 +1.0 +0.1
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