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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
basR and yjdB overlap by 4 nucleotides
yjdB and adiC are separated by 103 nucleotides
adiC and adiY are separated by 136 nucleotides
adiY and adiA are separated by 324 nucleotides
b4113: basR - DNA-binding response regulator in two-component regulatory system with BasS (NCBI), at 4,331,305 to 4,331,973
basR
b4114: yjdB - orf, hypothetical protein (VIMSS), at 4,331,970 to 4,333,613
yjdB
b4115: adiC - arginine:agmatin (NCBI), at 4,333,717 to 4,335,054
adiC
b4116: adiY - DNA-binding transcriptional activator (NCBI), at 4,335,191 to 4,335,952
adiY
b4117: adiA - biodegradative arginine decarboxylase (RefSeq), at 4,336,277 to 4,338,544
adiA
Group
Condition
b4113
b4114
b4115
b4116
b4117
basR
yjdB
adiC
adiY
adiA
stress
Aluminum chloride 5 mM
-3.3
-0.6
+1.6
-0.2
-0.1
control
PBS_cage_2_mouse_1_Cecum_40 hr
-0.7
-0.1
+0.1
-0.5
-0.3
phage
Phage_N4_cage_3_mouse_3_Cecum_40 hr
-0.2
-0.1
-0.0
-1.1
-0.1
gfp_control
GFP_control
-0.4
-0.3
-0.2
-0.0
-0.2
drt_type_3
DRT_type_3
-0.3
-0.2
-0.4
-0.1
-0.2
stress
LB Anaerobic with Zinc Pyrithione 0.0000025 mM
-0.6
-0.2
-0.0
-0.1
-0.3
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.0
-0.3
+0.0
-0.6
-0.2
phage
Phage_N4_cage_3_mouse_1_Cecum_40 hr
-0.2
+0.1
-0.1
-0.7
-0.1
stress
Ciprofloxacin Hydrochloride 0.0024 mM
-0.5
-0.1
-0.1
-0.3
+0.1
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.1
-0.5
+0.0
-0.5
-0.0
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.3
-0.8
-0.0
-0.2
-0.1
carbon source
Ying_Others16 rep A; time point 2
-0.7
+0.2
-0.2
+0.1
+0.1
motility
outer cut, LB soft agar motility assay
+0.5
-0.1
-0.1
-0.2
-0.2
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+0.4
+0.4
-0.1
-0.5
-0.1
carbon source
carbon source D-Mannose 0.5mM
+0.5
+0.4
-0.3
-0.6
+0.2
motility
outer cut, LB soft agar motility assay
+0.7
-0.0
-0.1
-0.3
-0.1
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+0.4
-0.2
-0.0
+0.5
-0.1
stress
LB Aerobic with Irgasan 0.0000003125 mM
+0.2
-0.1
-0.2
+0.7
+0.0
stress
limonene 5 vol%
+0.8
-0.1
+0.2
+0.0
-0.0
phage
RB68
-0.3
+0.2
+0.2
+0.6
-0.1
phage
RB68
-0.1
+0.3
+0.2
+0.6
+0.0
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
+1.2
+0.0
-0.2
+0.3
-0.1
phage
T7_phage 0.00015625 MOI TC4
-0.1
+0.5
+0.5
+0.3
+0.1
phage
N4_phage 0.009375 MOI TC2
+0.1
+0.3
+0.3
+0.5
+0.1
phage
R2_N4_T2_phage10
+0.1
+0.4
+0.5
+0.3
-0.0
phage
Ste41
+0.1
+0.4
+0.3
+0.6
-0.1
phage
Papa dilution 10-1 cacl2 maltose
+0.2
+0.1
+0.3
+0.8
-0.0
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
+0.4
+0.4
+0.7
+0.0
+0.0
phage
T7_phage 0.0015625 MOI TC1
+0.4
+0.3
+0.3
+0.2
+0.2
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
+0.7
+0.1
+0.4
+0.4
-0.0
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