Fitness for 5 genes in Escherichia coli BW25113

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500 ntbasR and yjdB overlap by 4 nucleotidesyjdB and adiC are separated by 103 nucleotidesadiC and adiY are separated by 136 nucleotidesadiY and adiA are separated by 324 nucleotides b4113: basR - DNA-binding response regulator in two-component regulatory system with BasS (NCBI), at 4,331,305 to 4,331,973 basR b4114: yjdB - orf, hypothetical protein (VIMSS), at 4,331,970 to 4,333,613 yjdB b4115: adiC - arginine:agmatin (NCBI), at 4,333,717 to 4,335,054 adiC b4116: adiY - DNA-binding transcriptional activator (NCBI), at 4,335,191 to 4,335,952 adiY b4117: adiA - biodegradative arginine decarboxylase (RefSeq), at 4,336,277 to 4,338,544 adiA
Group Condition b4113 b4114 b4115 b4116 b4117
basR yjdB adiC adiY adiA
stress Aluminum chloride 5 mM -3.3 -0.6 +1.6 -0.2 -0.1
control PBS_cage_2_mouse_1_Cecum_40 hr -0.7 -0.1 +0.1 -0.5 -0.3
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr -0.2 -0.1 -0.0 -1.1 -0.1
gfp_control GFP_control -0.4 -0.3 -0.2 -0.0 -0.2
drt_type_3 DRT_type_3 -0.3 -0.2 -0.4 -0.1 -0.2
stress LB Anaerobic with Zinc Pyrithione 0.0000025 mM -0.6 -0.2 -0.0 -0.1 -0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.0 -0.3 +0.0 -0.6 -0.2
phage Phage_N4_cage_3_mouse_1_Cecum_40 hr -0.2 +0.1 -0.1 -0.7 -0.1
stress Ciprofloxacin Hydrochloride 0.0024 mM -0.5 -0.1 -0.1 -0.3 +0.1
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.1 -0.5 +0.0 -0.5 -0.0
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.3 -0.8 -0.0 -0.2 -0.1
carbon source Ying_Others16 rep A; time point 2 -0.7 +0.2 -0.2 +0.1 +0.1
motility outer cut, LB soft agar motility assay +0.5 -0.1 -0.1 -0.2 -0.2
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +0.4 +0.4 -0.1 -0.5 -0.1
carbon source carbon source D-Mannose 0.5mM +0.5 +0.4 -0.3 -0.6 +0.2
motility outer cut, LB soft agar motility assay +0.7 -0.0 -0.1 -0.3 -0.1
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.4 -0.2 -0.0 +0.5 -0.1
stress LB Aerobic with Irgasan 0.0000003125 mM +0.2 -0.1 -0.2 +0.7 +0.0
stress limonene 5 vol% +0.8 -0.1 +0.2 +0.0 -0.0
phage RB68 -0.3 +0.2 +0.2 +0.6 -0.1
phage RB68 -0.1 +0.3 +0.2 +0.6 +0.0
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM +1.2 +0.0 -0.2 +0.3 -0.1
phage T7_phage 0.00015625 MOI TC4 -0.1 +0.5 +0.5 +0.3 +0.1
phage N4_phage 0.009375 MOI TC2 +0.1 +0.3 +0.3 +0.5 +0.1
phage R2_N4_T2_phage10 +0.1 +0.4 +0.5 +0.3 -0.0
phage Ste41 +0.1 +0.4 +0.3 +0.6 -0.1
phage Papa dilution 10-1 cacl2 maltose +0.2 +0.1 +0.3 +0.8 -0.0
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.4 +0.4 +0.7 +0.0 +0.0
phage T7_phage 0.0015625 MOI TC1 +0.4 +0.3 +0.3 +0.2 +0.2
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.7 +0.1 +0.4 +0.4 -0.0
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