Fitness for 5 genes in Escherichia coli BW25113

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500 ntyjdA and yjcZ overlap by 4 nucleotidesyjcZ and proP are separated by 263 nucleotidesproP and pmrR are separated by 111 nucleotidespmrR and basS overlap by 25 nucleotides b4109: yjdA - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 4,325,158 to 4,327,386 yjdA b4110: yjcZ - orf, hypothetical protein (VIMSS), at 4,327,383 to 4,328,261 yjcZ b4111: proP - proline/glycine betaine transporter (NCBI), at 4,328,525 to 4,330,027 proP b4703: pmrR - predicted regulator of BasS activity, membrane protein (NCBI), at 4,330,139 to 4,330,228 pmrR b4112: basS - sensory histidine kinase in two-component regulatory system with BasR (NCBI), at 4,330,204 to 4,331,295 basS
Group Condition b4109 b4110 b4111 b4703 b4112
yjdA yjcZ proP pmrR basS
stress Chloride 750 mM +0.0 +0.5 -4.2 N.D. -0.1
stress Aluminum chloride 5 mM -0.2 -0.9 +0.4 N.D. -1.3
phage T7_phage 0.00015625 MOI TC4 +0.0 -0.9 -0.0 N.D. -0.3
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -0.4 +0.1 -0.5 N.D. +0.2
phage RB68 -0.0 +0.3 -0.5 N.D. -0.3
phage RB68 +0.1 +0.2 -0.8 N.D. +0.1
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.4 -0.5 +0.1 N.D. -0.3
phage T7_phage 0.00015625 MOI TC3 +0.4 -0.2 -0.2 N.D. +0.3
stress limonene 5 vol% +0.0 -0.4 +0.1 N.D. +0.8
carbon source Ying_sugar7 rep C; time point 3 +0.3 +0.6 -0.2 N.D. -0.1
carbon source Ying_AminoAcid20 rep C; time point 3 +0.4 +0.6 -0.1 N.D. -0.1
protonated/deuterated deuterated glucose in H2O +0.3 +0.5 +0.1 N.D. +0.3
carbon source Ying_AminoAcid20 rep B; time point 3 +0.4 +0.7 +0.1 N.D. -0.0
stress Benzalkonium Chloride 0.004 mg/ml +0.1 +0.3 +0.5 N.D. +0.2
phage N4_phage 0.00009375 MOI TC4 +0.4 +0.5 +0.2 N.D. +0.1
phage N4_phage 0.009375 MOI TC2 +0.6 +0.4 +0.3 N.D. +0.2
carbon source Ying_Nucleobase14 rep C; time point 2 +0.8 +0.8 +0.0 N.D. -0.1
carbon source Ying_Nucleobase14 rep A; time point 2 +0.7 +0.9 +0.1 N.D. -0.0
carbon source Ying_Nucleobase14 rep B; time point 2 +0.6 +0.8 +0.0 N.D. +0.1
carbon source Ying_AminoAcid20 rep A; time point 3 +0.6 +0.8 -0.0 N.D. +0.2
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) +1.1 +0.9 -0.0 N.D. +0.1
carbon source Ying_Nucleobase14 rep C; time point 3 +1.1 +1.3 -0.2 N.D. -0.2
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) +1.2 +1.1 -0.0 N.D. +0.1
carbon source Ying_Nucleobase14 rep A; time point 3 +1.2 +1.4 -0.0 N.D. -0.0
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) +1.3 +1.3 -0.2 N.D. +0.3
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) +1.2 +1.3 +0.1 N.D. +0.1
stress octanol 0.08 vol% +0.5 +0.1 +2.0 N.D. +0.3
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) +1.5 +1.5 +0.0 N.D. +0.0
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) +1.4 +1.5 -0.1 N.D. +0.2
carbon source Ying_Nucleobase14 rep B; time point 3 +1.4 +1.5 +0.1 N.D. +0.1
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