Fitness for 5 genes in Escherichia coli BW25113

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500 ntphnB and phnA are separated by 657 nucleotidesphnA and yjdA are separated by 400 nucleotidesyjdA and yjcZ overlap by 4 nucleotidesyjcZ and proP are separated by 263 nucleotides b4107: phnB - hypothetical protein (NCBI), at 4,323,321 to 4,323,764 phnB b4108: phnA - hypothetical protein (NCBI), at 4,324,422 to 4,324,757 phnA b4109: yjdA - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 4,325,158 to 4,327,386 yjdA b4110: yjcZ - orf, hypothetical protein (VIMSS), at 4,327,383 to 4,328,261 yjcZ b4111: proP - proline/glycine betaine transporter (NCBI), at 4,328,525 to 4,330,027 proP
Group Condition b4107 b4108 b4109 b4110 b4111
phnB phnA yjdA yjcZ proP
stress Chloride 750 mM -0.4 -0.2 +0.0 +0.5 -4.2
phage R2_N4_T1_phage10 -0.5 -1.5 -0.2 -0.1 -0.1
carbon source carbon source D-Glucose 0.25mM -0.4 -1.1 +0.0 +0.1 +0.2
motility outer cut, LB soft agar motility assay -1.0 -0.6 -0.1 +0.6 -0.1
stress Aluminum chloride 5 mM +0.4 -0.3 -0.2 -0.9 +0.4
stress Cisplatin 0.1 mg/ml -0.6 +0.7 +0.0 -0.2 -0.3
carbon source carbon source D-Mannose 1mM +0.5 -1.1 +0.1 +0.1 +0.1
motility outer cut, LB soft agar motility assay +0.4 -1.2 -0.1 +0.5 +0.1
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.9 +0.4 +0.1 +0.2 -0.1
phage T7_phage 0.00015625 MOI TC4 +0.7 +0.1 +0.0 -0.9 -0.0
phage R2_N4_T2_phage10 +0.3 -1.1 +0.1 +0.5 +0.3
phage RB68 +0.3 +0.7 -0.0 +0.3 -0.5
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.3 +0.5 +0.4 -0.5 +0.1
phage Ste41 +0.9 +0.5 -0.1 -0.2 -0.1
carbon source Ying_Nucleobase14 rep C; time point 2 -0.3 -0.2 +0.8 +0.8 +0.0
carbon source Ying_Nucleobase14 rep B; time point 2 -0.1 -0.3 +0.6 +0.8 +0.0
carbon source Ying_AminoAcid20 rep A; time point 3 +0.1 -0.2 +0.6 +0.8 -0.0
carbon source Ying_Nucleobase14 rep A; time point 2 +0.0 -0.1 +0.7 +0.9 +0.1
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) -0.1 -0.6 +1.3 +1.3 -0.2
carbon source Ying_Nucleobase14 rep C; time point 3 +0.0 -0.4 +1.1 +1.3 -0.2
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) -0.0 +0.0 +1.1 +0.9 -0.0
carbon source Ying_Nucleobase14 rep A; time point 3 -0.1 -0.5 +1.2 +1.4 -0.0
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) -0.0 -0.3 +1.2 +1.1 -0.0
carbon source Ying_Nucleobase14 rep B; time point 3 -0.5 -0.6 +1.4 +1.5 +0.1
stress Benzalkonium Chloride 0.004 mg/ml +0.6 +0.7 +0.1 +0.3 +0.5
stress octanol 0.08 vol% -0.5 +0.2 +0.5 +0.1 +2.0
phage N4_phage 0.009375 MOI TC2 +0.6 +0.5 +0.6 +0.4 +0.3
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) +0.0 -0.6 +1.5 +1.5 +0.0
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) +0.3 -0.5 +1.2 +1.3 +0.1
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) +0.1 -0.3 +1.4 +1.5 -0.1
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