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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
phnC and phnB are separated by 132 nucleotides
phnB and phnA are separated by 657 nucleotides
phnA and yjdA are separated by 400 nucleotides
yjdA and yjcZ overlap by 4 nucleotides
b4106: phnC - phosphonate/organophosphate ester transporter subunit (NCBI), at 4,322,400 to 4,323,188
phnC
b4107: phnB - hypothetical protein (NCBI), at 4,323,321 to 4,323,764
phnB
b4108: phnA - hypothetical protein (NCBI), at 4,324,422 to 4,324,757
phnA
b4109: yjdA - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 4,325,158 to 4,327,386
yjdA
b4110: yjcZ - orf, hypothetical protein (VIMSS), at 4,327,383 to 4,328,261
yjcZ
Group
Condition
b4106
b4107
b4108
b4109
b4110
phnC
phnB
phnA
yjdA
yjcZ
phage
R2_N4_T1_phage10
-0.3
-0.5
-1.5
-0.2
-0.1
motility
outer cut, LB soft agar motility assay
-1.0
+0.4
-1.2
-0.1
+0.5
carbon source
carbon source D-Glucose 0.25mM
+0.4
-0.4
-1.1
+0.0
+0.1
stress
Aluminum chloride 5 mM
+0.2
+0.4
-0.3
-0.2
-0.9
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.9
+0.3
+0.2
-0.2
-0.2
motility
outer cut, LB soft agar motility assay
+0.4
-1.0
-0.6
-0.1
+0.6
carbon source
carbon source D-Mannose 1mM
+0.2
+0.5
-1.1
+0.1
+0.1
phage
R2_N4_T2_phage10
+0.2
+0.3
-1.1
+0.1
+0.5
micoeukaryotes
Tetrahymena sp., Mixed culture-1
+0.2
-0.9
+0.4
+0.1
+0.2
stress
Cisplatin 0.1 mg/ml
+0.5
-0.6
+0.7
+0.0
-0.2
carbon source
Ying_Nucleobase14 rep C; time point 2
-0.3
-0.3
-0.2
+0.8
+0.8
phage
Ste41
-0.3
+0.9
+0.5
-0.1
-0.2
carbon source
carbon source D-Mannose 5mM
-0.6
+0.8
+0.4
+0.1
+0.2
carbon source
carbon source D-Glucose 0.25mM
+0.6
-0.2
+0.8
-0.4
+0.1
carbon source
Ying_Nucleobase14 rep A; time point 2
-0.5
+0.0
-0.1
+0.7
+0.9
carbon source
Ying_AminoAcid20 rep A; time point 3
-0.2
+0.1
-0.2
+0.6
+0.8
carbon source
Ying_Nucleobase14 rep B; time point 3
-0.5
-0.5
-0.6
+1.4
+1.5
carbon source
LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
-0.3
-0.0
-0.3
+1.2
+1.1
carbon source
LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
-0.3
-0.0
+0.0
+1.1
+0.9
carbon source
Ying_Nucleobase14 rep C; time point 3
-0.4
+0.0
-0.4
+1.1
+1.3
stress
Benzalkonium Chloride 0.004 mg/ml
-0.0
+0.6
+0.7
+0.1
+0.3
carbon source
Ying_Nucleobase14 rep A; time point 3
-0.2
-0.1
-0.5
+1.2
+1.4
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.6
+0.5
+0.9
-0.1
-0.0
carbon source
LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C)
+0.0
-0.1
-0.6
+1.3
+1.3
phage
N4_phage 0.00009375 MOI TC4
+0.8
+0.4
+0.0
+0.4
+0.5
carbon source
LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C)
-0.2
+0.3
-0.5
+1.2
+1.3
carbon source
LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
-0.3
+0.0
-0.6
+1.5
+1.5
carbon source
LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C)
-0.0
+0.1
-0.3
+1.4
+1.5
phage
N4_phage 0.009375 MOI TC2
+0.8
+0.6
+0.5
+0.6
+0.4
stress
Phosphomycin 0.001 mg/ml
+4.9
-0.3
+0.2
+0.1
+0.0
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