Fitness for 5 genes in Escherichia coli BW25113

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500 ntphnF and phnE are separated by 20 nucleotidesphnE and phnD are separated by 521 nucleotidesphnD and phnC are separated by 24 nucleotidesphnC and phnB are separated by 132 nucleotides b4102: phnF - predicted DNA-binding transcriptional regulator of phosphonate uptake and biodegradation (NCBI), at 4,319,720 to 4,320,445 phnF b4104: phnE - phosphonate/organophosphate ester transporter subunit (NCBI), at 4,320,466 to 4,320,837 phnE b4105: phnD - phosphonate/organophosphate ester transporter subunit (NCBI), at 4,321,359 to 4,322,375 phnD b4106: phnC - phosphonate/organophosphate ester transporter subunit (NCBI), at 4,322,400 to 4,323,188 phnC b4107: phnB - hypothetical protein (NCBI), at 4,323,321 to 4,323,764 phnB
Group Condition b4102 b4104 b4105 b4106 b4107
phnF phnE phnD phnC phnB
phage T7_phage 0.0015625 MOI TC2 -1.5 -3.2 -0.1 -0.6 +0.2
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM -1.1 -1.0 -0.1 -0.8 +0.2
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM -0.8 -1.1 -0.5 -0.5 +0.1
stress 3-methyl-3-butenol 0.625 vol% -0.6 -0.7 -0.2 -0.4 -0.4
carbon source Ying_Nucleobase14 rep B; time point 3 -0.2 -1.1 +0.1 -0.5 -0.5
stress pinene 5 vol% -0.3 -0.9 -0.2 -0.3 -0.4
stress 3-methyl-1-butanol 1.25 vol% -0.4 -0.9 +0.1 -0.1 -0.7
msc 22hr_mediaonly_rep1_lowinoculum -0.3 -1.0 +0.3 -0.5 -0.3
control PBS_cage_2_mouse_1_Cecum_40 hr -0.4 -0.8 -0.5 -0.5 +0.5
motility outer cut, LB soft agar motility assay +0.2 -0.4 -0.8 +0.4 -1.0
gfp_control GFP_control -1.1 -0.8 +0.3 -0.3 +0.4
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr -0.6 +1.0 -0.5 -0.8 -0.1
retron_ec_48_control retron_Ec_48_control -0.8 -0.5 +0.4 -0.3 +0.2
gfp_control GFP_control -0.6 -0.7 +0.3 -0.1 +0.3
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.8 -0.3 -0.3 +0.1 +0.6
sir2-hera SIR2-HerA -0.6 -0.6 +0.6 -0.2 +0.2
control PBS_cage_1_mouse_2_Cecum_40 hr -0.4 -0.4 -0.6 +0.0 +0.7
mzaabcde mzaABCDE -0.6 -0.6 +0.5 -0.1 +0.3
stress Cisplatin 0.1 mg/ml -0.8 +1.0 -0.5 +0.5 -0.6
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.1 +1.0 -0.3 +0.2 -0.9
stress Ciprofloxacin Hydrochloride 0.0003 mM +0.4 +0.3 -0.3 +0.5 -0.5
phage T7_phage 0.00015625 MOI TC4 +0.4 +0.3 -0.8 +0.0 +0.7
motility outer cut, LB soft agar motility assay +0.2 +0.5 +0.5 -1.0 +0.4
control PBS_cage_1_mouse_1_Cecum_40 hr -0.5 +0.8 -0.6 +0.1 +0.9
carbon source carbon source D-Glucose 0.25mM -0.5 +0.9 +0.4 +0.4 -0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.6 -0.5 +0.2 +0.6 +0.5
phage N4_phage 0.00009375 MOI TC4 +0.3 +0.6 +0.7 +0.8 +0.4
phage N4_phage 0.009375 MOI TC2 +0.8 +0.5 +0.4 +0.8 +0.6
phage Papa dilution 10-1 cacl2 maltose +1.3 +1.0 +0.4 +0.4 -0.0
stress Phosphomycin 0.001 mg/ml +8.6 +9.5 +4.5 +4.9 -0.3
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