Fitness for 5 genes in Escherichia coli BW25113

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500 ntphnG and phnF are separated by 0 nucleotidesphnF and phnE are separated by 20 nucleotidesphnE and phnD are separated by 521 nucleotidesphnD and phnC are separated by 24 nucleotides b4101: phnG - carbon-phosphorus lyase complex subunit (NCBI), at 4,319,267 to 4,319,719 phnG b4102: phnF - predicted DNA-binding transcriptional regulator of phosphonate uptake and biodegradation (NCBI), at 4,319,720 to 4,320,445 phnF b4104: phnE - phosphonate/organophosphate ester transporter subunit (NCBI), at 4,320,466 to 4,320,837 phnE b4105: phnD - phosphonate/organophosphate ester transporter subunit (NCBI), at 4,321,359 to 4,322,375 phnD b4106: phnC - phosphonate/organophosphate ester transporter subunit (NCBI), at 4,322,400 to 4,323,188 phnC
Group Condition b4101 b4102 b4104 b4105 b4106
phnG phnF phnE phnD phnC
phage T7_phage 0.0015625 MOI TC2 -0.2 -1.5 -3.2 -0.1 -0.6
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM -1.9 -1.1 -1.0 -0.1 -0.8
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM -1.6 -0.8 -1.1 -0.5 -0.5
carbon source Ying_Nucleobase14 rep B; time point 3 -2.0 -0.2 -1.1 +0.1 -0.5
carbon source Ying_Nucleobase14 rep A; time point 2 -1.6 -0.3 -0.6 -0.1 -0.5
carbon source Ying_Nucleobase14 rep B; time point 2 -1.6 -0.6 -0.4 -0.2 -0.0
stress Spectinomycin 0.00625 mg/ml -1.2 -0.3 -0.4 -0.2 -0.6
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -1.2 -0.0 +0.1 -0.6 -0.9
carbon source Ying_Nucleobase14 rep A; time point 3 -1.2 -0.6 -0.4 -0.0 -0.2
carbon source Ying_Nucleobase14 rep C; time point 3 -1.4 -0.3 -0.1 -0.2 -0.4
stress 3-methyl-3-butenol 0.625 vol% -0.5 -0.6 -0.7 -0.2 -0.4
carbon source Ying_Nucleobase14 rep C; time point 2 -1.1 -0.4 -0.4 -0.1 -0.3
stress Spectinomycin 0.0125 mg/ml -1.5 -0.3 -0.5 -0.0 +0.1
gfp_control GFP_control -0.3 -1.1 -0.8 +0.3 -0.3
msc 22hr_mediaonly_rep1_lowinoculum -0.5 -0.3 -1.0 +0.3 -0.5
motility outer cut, LB soft agar motility assay -1.4 +0.2 -0.4 -0.8 +0.4
stress LB Aerobic with Ramoplanin 0.000125 mM -2.4 +0.3 +0.2 +0.0 -0.1
phage Lambda_ci1857 -1.2 -0.1 -0.8 +0.2 +0.0
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.8 -0.5 -0.5 +0.3 -0.3
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr -0.2 -0.6 +1.0 -0.5 -0.8
stress Cisplatin 0.1 mg/ml +0.1 -0.8 +1.0 -0.5 +0.5
carbon source carbon source D-Glucose 0.25mM -0.3 -0.5 +0.9 +0.4 +0.4
motility outer cut, LB soft agar motility assay +0.7 +0.2 +0.5 +0.5 -1.0
carbon source Dodecanol and Rhamnolipid (C) +0.9 +0.5 +0.2 +0.1 -0.7
stress LB Aerobic with Irgasan 0.0000003125 mM +1.0 +0.8 -0.2 -0.1 +0.2
phage N4_phage 0.00009375 MOI TC4 +0.2 +0.3 +0.6 +0.7 +0.8
plasmid transformation vcDART vector transformation; agar assay +1.0 +0.3 +0.6 +0.2 +0.4
phage N4_phage 0.009375 MOI TC2 +0.6 +0.8 +0.5 +0.4 +0.8
phage Papa dilution 10-1 cacl2 maltose +0.9 +1.3 +1.0 +0.4 +0.4
stress Phosphomycin 0.001 mg/ml +10.2 +8.6 +9.5 +4.5 +4.9
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