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Cofit
Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
phnG and phnF are separated by 0 nucleotides
phnF and phnE are separated by 20 nucleotides
phnE and phnD are separated by 521 nucleotides
phnD and phnC are separated by 24 nucleotides
b4101: phnG - carbon-phosphorus lyase complex subunit (NCBI), at 4,319,267 to 4,319,719
phnG
b4102: phnF - predicted DNA-binding transcriptional regulator of phosphonate uptake and biodegradation (NCBI), at 4,319,720 to 4,320,445
phnF
b4104: phnE - phosphonate/organophosphate ester transporter subunit (NCBI), at 4,320,466 to 4,320,837
phnE
b4105: phnD - phosphonate/organophosphate ester transporter subunit (NCBI), at 4,321,359 to 4,322,375
phnD
b4106: phnC - phosphonate/organophosphate ester transporter subunit (NCBI), at 4,322,400 to 4,323,188
phnC
Group
Condition
b4101
b4102
b4104
b4105
b4106
phnG
phnF
phnE
phnD
phnC
phage
T7_phage 0.0015625 MOI TC2
-0.2
-1.5
-3.2
-0.1
-0.6
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
-1.9
-1.1
-1.0
-0.1
-0.8
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
-1.6
-0.8
-1.1
-0.5
-0.5
carbon source
Ying_Nucleobase14 rep B; time point 3
-2.0
-0.2
-1.1
+0.1
-0.5
carbon source
Ying_Nucleobase14 rep A; time point 2
-1.6
-0.3
-0.6
-0.1
-0.5
carbon source
Ying_Nucleobase14 rep B; time point 2
-1.6
-0.6
-0.4
-0.2
-0.0
stress
Spectinomycin 0.00625 mg/ml
-1.2
-0.3
-0.4
-0.2
-0.6
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-1.2
-0.0
+0.1
-0.6
-0.9
carbon source
Ying_Nucleobase14 rep A; time point 3
-1.2
-0.6
-0.4
-0.0
-0.2
carbon source
Ying_Nucleobase14 rep C; time point 3
-1.4
-0.3
-0.1
-0.2
-0.4
stress
3-methyl-3-butenol 0.625 vol%
-0.5
-0.6
-0.7
-0.2
-0.4
carbon source
Ying_Nucleobase14 rep C; time point 2
-1.1
-0.4
-0.4
-0.1
-0.3
stress
Spectinomycin 0.0125 mg/ml
-1.5
-0.3
-0.5
-0.0
+0.1
gfp_control
GFP_control
-0.3
-1.1
-0.8
+0.3
-0.3
msc
22hr_mediaonly_rep1_lowinoculum
-0.5
-0.3
-1.0
+0.3
-0.5
motility
outer cut, LB soft agar motility assay
-1.4
+0.2
-0.4
-0.8
+0.4
stress
LB Aerobic with Ramoplanin 0.000125 mM
-2.4
+0.3
+0.2
+0.0
-0.1
phage
Lambda_ci1857
-1.2
-0.1
-0.8
+0.2
+0.0
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-0.8
-0.5
-0.5
+0.3
-0.3
phage
Phage_N4_cage_3_mouse_3_Cecum_40 hr
-0.2
-0.6
+1.0
-0.5
-0.8
stress
Cisplatin 0.1 mg/ml
+0.1
-0.8
+1.0
-0.5
+0.5
carbon source
carbon source D-Glucose 0.25mM
-0.3
-0.5
+0.9
+0.4
+0.4
motility
outer cut, LB soft agar motility assay
+0.7
+0.2
+0.5
+0.5
-1.0
carbon source
Dodecanol and Rhamnolipid (C)
+0.9
+0.5
+0.2
+0.1
-0.7
stress
LB Aerobic with Irgasan 0.0000003125 mM
+1.0
+0.8
-0.2
-0.1
+0.2
phage
N4_phage 0.00009375 MOI TC4
+0.2
+0.3
+0.6
+0.7
+0.8
plasmid transformation
vcDART vector transformation; agar assay
+1.0
+0.3
+0.6
+0.2
+0.4
phage
N4_phage 0.009375 MOI TC2
+0.6
+0.8
+0.5
+0.4
+0.8
phage
Papa dilution 10-1 cacl2 maltose
+0.9
+1.3
+1.0
+0.4
+0.4
stress
Phosphomycin 0.001 mg/ml
+10.2
+8.6
+9.5
+4.5
+4.9
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