Fitness for 5 genes in Escherichia coli BW25113

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500 ntnrfF and nrfG overlap by 4 nucleotidesnrfG and gltP are separated by 341 nucleotidesgltP and yjcO are separated by 641 nucleotidesyjcO and fdhF are separated by 93 nucleotides b4075: nrfF - heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfF (NCBI), at 4,291,186 to 4,291,569 nrfF b4076: nrfG - heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG (NCBI), at 4,291,566 to 4,292,162 nrfG b4077: gltP - glutamate/aspartate:proton symporter (NCBI), at 4,292,504 to 4,293,817 gltP b4078: yjcO - hypothetical protein (NCBI), at 4,294,459 to 4,295,148 yjcO b4079: fdhF - formate dehydrogenase-H, selenopolypeptide subunit (NCBI), at 4,295,242 to 4,297,389 fdhF
Group Condition b4075 b4076 b4077 b4078 b4079
nrfF nrfG gltP yjcO fdhF
stress Benzalkonium Chloride 0.004 mg/ml -0.6 -2.0 +0.3 -0.2 +0.2
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.0 -0.8 +0.2 -1.1 -0.3
stress Chloramphenicol 0.001 mg/ml -0.2 -1.1 +0.1 -0.5 +0.0
stress Chloramphenicol 0.005 mg/ml -0.7 -0.6 +0.0 -0.1 -0.2
stress Chloramphenicol 0.0025 mg/ml -0.4 -1.0 +0.3 -0.3 +0.1
nophagecontrol R1_N4_T1_phage0 +0.2 -0.9 -0.3 -0.3 -0.0
stress 3-methyl-1-butanol 1.25 vol% -0.4 -1.2 +0.1 +0.0 +0.2
stress LB Anaerobic with Spiramycin 0.000125 mM -0.7 -0.3 +0.2 -0.3 +0.1
motility outer cut, LB soft agar motility assay +0.2 -0.2 -0.1 -0.3 -0.6
stress Bacitracin 1 mg/ml -0.0 -0.7 +0.7 -0.7 -0.3
stress LB Aerobic with Spiramycin 0.0000625 mM -0.6 -0.4 +0.3 -0.3 +0.1
stress LB Anaerobic with Furaltadone 0.000015625 mM -0.5 +0.4 -0.1 -0.4 -0.3
control PBS_cage_1_mouse_1_Cecum_40 hr -0.8 -0.6 +0.0 +0.2 +0.2
phage Lambda_ci1857 -0.4 +0.3 -0.5 -0.2 +0.1
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.6 -0.2 +0.3 +0.2 -0.3
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +0.1 -0.6 +0.3 -0.4 -0.0
carbon source D-Glucosamine Hydrochloride (C) -0.7 -0.4 +0.4 +0.2 -0.1
carbon source Glycolic Acid (C) -0.7 -0.4 +0.3 +0.1 +0.1
stress Ciprofloxacin Hydrochloride 0.0024 mM -0.5 +0.3 +0.3 -0.3 -0.3
phage Ste41 +0.8 -0.5 -0.1 -0.7 +0.1
carbon source Glycolic Acid (C) -0.5 -0.4 +0.5 -0.0 +0.1
carbon source acetate (C) -0.8 -0.2 +0.2 +0.3 +0.0
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.4 -0.4 +0.1 +0.3 -0.5
phage T7_phage 0.00015625 MOI TC4 +0.8 -0.0 -0.1 -0.5 -0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.5 +0.1 -0.3 +0.3 +0.5
carbon source a-Ketoglutaric (C) -0.3 -0.3 +0.2 +0.5 -0.1
stress Trimethoprim 0.0025 mM -0.2 -0.8 +0.7 +0.4 +0.1
motility inner cut, LB soft agar motility assay +0.4 -0.9 +0.4 +0.4 +0.1
motility outer cut, LB soft agar motility assay +0.1 +1.0 -0.1 -0.2 -0.2
stress Trimethoprim 0.00125 mM -0.1 -0.1 +0.7 +0.6 +0.2
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