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Protein
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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
nrfF and nrfG overlap by 4 nucleotides
nrfG and gltP are separated by 341 nucleotides
gltP and yjcO are separated by 641 nucleotides
yjcO and fdhF are separated by 93 nucleotides
b4075: nrfF - heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfF (NCBI), at 4,291,186 to 4,291,569
nrfF
b4076: nrfG - heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG (NCBI), at 4,291,566 to 4,292,162
nrfG
b4077: gltP - glutamate/aspartate:proton symporter (NCBI), at 4,292,504 to 4,293,817
gltP
b4078: yjcO - hypothetical protein (NCBI), at 4,294,459 to 4,295,148
yjcO
b4079: fdhF - formate dehydrogenase-H, selenopolypeptide subunit (NCBI), at 4,295,242 to 4,297,389
fdhF
Group
Condition
b4075
b4076
b4077
b4078
b4079
nrfF
nrfG
gltP
yjcO
fdhF
stress
Benzalkonium Chloride 0.004 mg/ml
-0.6
-2.0
+0.3
-0.2
+0.2
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.0
-0.8
+0.2
-1.1
-0.3
stress
Chloramphenicol 0.001 mg/ml
-0.2
-1.1
+0.1
-0.5
+0.0
stress
Chloramphenicol 0.005 mg/ml
-0.7
-0.6
+0.0
-0.1
-0.2
stress
Chloramphenicol 0.0025 mg/ml
-0.4
-1.0
+0.3
-0.3
+0.1
nophagecontrol
R1_N4_T1_phage0
+0.2
-0.9
-0.3
-0.3
-0.0
stress
3-methyl-1-butanol 1.25 vol%
-0.4
-1.2
+0.1
+0.0
+0.2
stress
LB Anaerobic with Spiramycin 0.000125 mM
-0.7
-0.3
+0.2
-0.3
+0.1
motility
outer cut, LB soft agar motility assay
+0.2
-0.2
-0.1
-0.3
-0.6
stress
Bacitracin 1 mg/ml
-0.0
-0.7
+0.7
-0.7
-0.3
stress
LB Aerobic with Spiramycin 0.0000625 mM
-0.6
-0.4
+0.3
-0.3
+0.1
stress
LB Anaerobic with Furaltadone 0.000015625 mM
-0.5
+0.4
-0.1
-0.4
-0.3
control
PBS_cage_1_mouse_1_Cecum_40 hr
-0.8
-0.6
+0.0
+0.2
+0.2
phage
Lambda_ci1857
-0.4
+0.3
-0.5
-0.2
+0.1
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.6
-0.2
+0.3
+0.2
-0.3
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
+0.1
-0.6
+0.3
-0.4
-0.0
carbon source
D-Glucosamine Hydrochloride (C)
-0.7
-0.4
+0.4
+0.2
-0.1
carbon source
Glycolic Acid (C)
-0.7
-0.4
+0.3
+0.1
+0.1
stress
Ciprofloxacin Hydrochloride 0.0024 mM
-0.5
+0.3
+0.3
-0.3
-0.3
phage
Ste41
+0.8
-0.5
-0.1
-0.7
+0.1
carbon source
Glycolic Acid (C)
-0.5
-0.4
+0.5
-0.0
+0.1
carbon source
acetate (C)
-0.8
-0.2
+0.2
+0.3
+0.0
micoeukaryotes
Tetrahymena sp., Mixed culture-1
+0.4
-0.4
+0.1
+0.3
-0.5
phage
T7_phage 0.00015625 MOI TC4
+0.8
-0.0
-0.1
-0.5
-0.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.5
+0.1
-0.3
+0.3
+0.5
carbon source
a-Ketoglutaric (C)
-0.3
-0.3
+0.2
+0.5
-0.1
stress
Trimethoprim 0.0025 mM
-0.2
-0.8
+0.7
+0.4
+0.1
motility
inner cut, LB soft agar motility assay
+0.4
-0.9
+0.4
+0.4
+0.1
motility
outer cut, LB soft agar motility assay
+0.1
+1.0
-0.1
-0.2
-0.2
stress
Trimethoprim 0.00125 mM
-0.1
-0.1
+0.7
+0.6
+0.2
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