Fitness for 5 genes in Escherichia coli BW25113

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500 ntactP and yjcH overlap by 4 nucleotidesyjcH and acs are separated by 199 nucleotidesacs and nrfA are separated by 392 nucleotidesnrfA and nrfB are separated by 44 nucleotides b4067: actP - acetate permease (NCBI), at 4,281,276 to 4,282,925 actP b4068: yjcH - required for glycolate transport, with actP (from data), at 4,282,922 to 4,283,236 yjcH b4069: acs - acetyl-coenzyme A synthetase (NCBI), at 4,283,436 to 4,285,394 acs b4070: nrfA - nitrite reductase, formate-dependent, cytochrome (NCBI), at 4,285,787 to 4,287,223 nrfA b4071: nrfB - formate-dependent nitrite reductase; a penta-haeme cytochrome c (VIMSS), at 4,287,268 to 4,287,834 nrfB
Group Condition b4067 b4068 b4069 b4070 b4071
actP yjcH acs nrfA nrfB
carbon source Glycolic Acid (C) -1.5 -2.3 -2.3 -0.2 +0.0
predation 6 hr rep1_HD100_10fold_dilution_grewON -0.5 -1.9 -0.7 -0.1 +0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.2 +0.2 -0.8 +0.0 -1.3
phage T7_phage 0.00015625 MOI TC3 -0.2 -1.9 -0.3 +0.3 +0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.2 -0.0 -1.0 +0.2 -0.7
carbon source carbon source D-Glucose 0.25mM +0.4 -1.8 -0.1 +0.3 -0.4
carbon source carbon source D-Mannose 5mM +0.1 -2.1 +0.3 +0.3 +0.2
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.1 -1.2 -0.3 -0.3 +0.6
carbon source D-Lactate (C) -0.3 -0.5 -0.4 +0.1 +0.9
carbon source acetate (C) +0.3 -0.4 -1.7 +0.1 +1.8
phage Ste41 -0.6 +0.8 -0.4 -0.2 +0.4
carbon source Ying_Others16 rep A; time point 2 -0.2 -0.5 -0.3 +0.2 +1.1
carbon source succinate (C) +0.0 -0.7 -0.6 +0.0 +1.5
carbon source L-Malic (C) -0.0 -0.2 -1.0 -0.0 +1.5
carbon source L-Malic (C) -0.0 -0.5 -0.8 +0.2 +1.5
stress octanol 0.08 vol% -0.2 +1.3 +0.2 +0.2 -1.0
carbon source acetate (C) +0.4 +0.0 -2.1 +0.1 +2.2
carbon source Ying_OrganicAcid7 rep B; time point 3 +0.0 -0.3 -0.5 -0.0 +1.5
carbon source Ying_OrganicAcid7 rep C; time point 3 +0.2 -0.7 -0.4 +0.1 +1.5
carbon source succinate (C) -0.1 -0.3 -0.8 +0.0 +1.9
carbon source Ying_OrganicAcid7 rep A; time point 3 +0.1 -0.3 -0.4 +0.1 +1.5
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) +0.3 -0.5 +0.1 +0.0 +1.5
carbon source a-Ketoglutaric (C) +0.1 -0.4 -0.3 +0.1 +2.3
carbon source a-Ketoglutaric (C) +0.2 -0.3 -0.3 +0.2 +2.1
carbon source Acetate (C) +0.6 +0.1 -0.5 -0.0 +2.5
M9 Acetate 0.5% aerobic +0.4 -0.0 -0.1 +0.0 +2.4
M9 Acetate 0.5% aerobic +0.5 +0.1 -0.1 -0.2 +2.5
carbon source Acetate (C) +0.5 +0.1 -0.5 +0.1 +2.6
nitrogen source L-Arginine (N) +0.7 -0.5 +0.0 -0.0 +3.2
nitrogen source L-Arginine (N) +0.5 +0.1 -0.2 +0.1 +3.4
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