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Protein
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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
aphA and yjbQ are separated by 110 nucleotides
yjbQ and yjbR are separated by 3 nucleotides
yjbR and uvrA are separated by 34 nucleotides
uvrA and ssb are separated by 253 nucleotides
b4055: aphA - acid phosphatase/phosphotransferase, class B, non-specific (NCBI), at 4,267,437 to 4,268,150
aphA
b4056: yjbQ - hypothetical protein (NCBI), at 4,268,261 to 4,268,677
yjbQ
b4057: yjbR - hypothetical protein (NCBI), at 4,268,681 to 4,269,037
yjbR
b4058: uvrA - excinuclease ABC subunit A (NCBI), at 4,269,072 to 4,271,894
uvrA
b4059: ssb - single-strand DNA-binding protein (NCBI), at 4,272,148 to 4,272,684
ssb
Group
Condition
b4055
b4056
b4057
b4058
b4059
aphA
yjbQ
yjbR
uvrA
ssb
stress
Cisplatin 0.1 mg/ml
+0.1
-0.1
-0.0
-5.6
N.D.
stress
LB Anaerobic with Furaltadone 0.000015625 mM
+0.1
-0.0
+0.2
-5.5
N.D.
stress
LB Aerobic with Nitrofurantoin 0.00003125 mM
+0.3
+0.0
-0.2
-5.3
N.D.
stress
Cisplatin 0.1 mg/ml
-0.2
-0.6
-0.2
-3.6
N.D.
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
+0.2
-0.0
+0.6
-5.3
N.D.
stress
LB Anaerobic with Nitrofurantoin 0.000015625 mM
-0.0
+0.1
-0.3
-4.3
N.D.
stress
LB Anaerobic with Furaltadone 0.000015625 mM
+0.2
+0.2
+0.1
-4.9
N.D.
stress
Cisplatin 0.1 mg/ml
+0.2
+0.2
-0.5
-4.3
N.D.
stress
2-nitrobenzenesulfonyl fluoride
+0.2
-0.3
-0.0
-3.7
N.D.
stress
Cisplatin 0.1 mg/ml
-0.1
-0.1
+0.4
-3.5
N.D.
stress
Cisplatin 0.05 mg/ml
+0.3
+0.0
-0.2
-3.4
N.D.
stress
Cisplatin 0.1 mg/ml
+0.2
-0.3
+0.2
-2.9
N.D.
stress
Cisplatin 0.05 mg/ml
+0.1
-0.2
-0.3
-2.3
N.D.
stress
LB Anaerobic with Nitrofurantoin 0.000015625 mM
+0.1
-0.4
-0.1
-2.2
N.D.
stress
LB Anaerobic with Furaltadone 0.00000390625 mM
+0.1
+0.1
-0.2
-2.5
N.D.
stress
Cisplatin 0.05 mg/ml
-0.0
-0.0
-0.1
-2.1
N.D.
stress
Cisplatin 0.025 mg/ml
+0.2
-0.1
+0.2
-2.6
N.D.
stress
3-methyl-3-butenol 0.625 vol%
+0.1
-0.1
+0.2
-2.4
N.D.
stress
3-methyl-3-butenol 0.625 vol%
-0.1
+0.2
+0.1
-2.2
N.D.
stress
Cisplatin 0.05 mg/ml
+0.0
-0.1
-0.1
-1.9
N.D.
stress
LB Anaerobic with Furaltadone 0.00000390625 mM
-0.0
+0.1
+0.3
-1.8
N.D.
carbon source
carbon source D-Mannose 5mM
+0.1
-0.1
-1.8
+0.4
N.D.
carbon source
a-Ketoglutaric (C)
-0.0
+0.7
-1.4
-0.6
N.D.
stress
Aluminum chloride 5 mM
-1.2
+0.1
-0.5
+0.4
N.D.
stress
3-methyl-2-butenol 1.25 vol%
-0.2
+0.4
+0.2
-1.5
N.D.
motility
outer cut, LB soft agar motility assay
+0.6
-0.6
-0.8
-0.2
N.D.
stress
Ciprofloxacin Hydrochloride 0.0024 mM
+0.4
-0.1
-1.3
+0.2
N.D.
carbon source
a-Ketoglutaric (C)
+0.1
+0.7
-1.0
-0.6
N.D.
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-1.1
-0.0
+0.7
+0.8
N.D.
stress
2-Furfuraldehyde 0.25 vol%
+0.1
+0.3
-0.2
+1.3
N.D.
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