Fitness for 5 genes in Escherichia coli BW25113

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500 ntaphA and yjbQ are separated by 110 nucleotidesyjbQ and yjbR are separated by 3 nucleotidesyjbR and uvrA are separated by 34 nucleotidesuvrA and ssb are separated by 253 nucleotides b4055: aphA - acid phosphatase/phosphotransferase, class B, non-specific (NCBI), at 4,267,437 to 4,268,150 aphA b4056: yjbQ - hypothetical protein (NCBI), at 4,268,261 to 4,268,677 yjbQ b4057: yjbR - hypothetical protein (NCBI), at 4,268,681 to 4,269,037 yjbR b4058: uvrA - excinuclease ABC subunit A (NCBI), at 4,269,072 to 4,271,894 uvrA b4059: ssb - single-strand DNA-binding protein (NCBI), at 4,272,148 to 4,272,684 ssb
Group Condition b4055 b4056 b4057 b4058 b4059
aphA yjbQ yjbR uvrA ssb
stress Cisplatin 0.1 mg/ml +0.1 -0.1 -0.0 -5.6 N.D.
stress LB Anaerobic with Furaltadone 0.000015625 mM +0.1 -0.0 +0.2 -5.5 N.D.
stress LB Aerobic with Nitrofurantoin 0.00003125 mM +0.3 +0.0 -0.2 -5.3 N.D.
stress Cisplatin 0.1 mg/ml -0.2 -0.6 -0.2 -3.6 N.D.
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +0.2 -0.0 +0.6 -5.3 N.D.
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM -0.0 +0.1 -0.3 -4.3 N.D.
stress LB Anaerobic with Furaltadone 0.000015625 mM +0.2 +0.2 +0.1 -4.9 N.D.
stress Cisplatin 0.1 mg/ml +0.2 +0.2 -0.5 -4.3 N.D.
stress 2-nitrobenzenesulfonyl fluoride +0.2 -0.3 -0.0 -3.7 N.D.
stress Cisplatin 0.1 mg/ml -0.1 -0.1 +0.4 -3.5 N.D.
stress Cisplatin 0.05 mg/ml +0.3 +0.0 -0.2 -3.4 N.D.
stress Cisplatin 0.1 mg/ml +0.2 -0.3 +0.2 -2.9 N.D.
stress Cisplatin 0.05 mg/ml +0.1 -0.2 -0.3 -2.3 N.D.
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM +0.1 -0.4 -0.1 -2.2 N.D.
stress LB Anaerobic with Furaltadone 0.00000390625 mM +0.1 +0.1 -0.2 -2.5 N.D.
stress Cisplatin 0.05 mg/ml -0.0 -0.0 -0.1 -2.1 N.D.
stress Cisplatin 0.025 mg/ml +0.2 -0.1 +0.2 -2.6 N.D.
stress 3-methyl-3-butenol 0.625 vol% +0.1 -0.1 +0.2 -2.4 N.D.
stress 3-methyl-3-butenol 0.625 vol% -0.1 +0.2 +0.1 -2.2 N.D.
stress Cisplatin 0.05 mg/ml +0.0 -0.1 -0.1 -1.9 N.D.
stress LB Anaerobic with Furaltadone 0.00000390625 mM -0.0 +0.1 +0.3 -1.8 N.D.
carbon source carbon source D-Mannose 5mM +0.1 -0.1 -1.8 +0.4 N.D.
carbon source a-Ketoglutaric (C) -0.0 +0.7 -1.4 -0.6 N.D.
stress Aluminum chloride 5 mM -1.2 +0.1 -0.5 +0.4 N.D.
stress 3-methyl-2-butenol 1.25 vol% -0.2 +0.4 +0.2 -1.5 N.D.
motility outer cut, LB soft agar motility assay +0.6 -0.6 -0.8 -0.2 N.D.
stress Ciprofloxacin Hydrochloride 0.0024 mM +0.4 -0.1 -1.3 +0.2 N.D.
carbon source a-Ketoglutaric (C) +0.1 +0.7 -1.0 -0.6 N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.1 -0.0 +0.7 +0.8 N.D.
stress 2-Furfuraldehyde 0.25 vol% +0.1 +0.3 -0.2 +1.3 N.D.
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