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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
malG and malF are separated by 14 nucleotides
malF and malE are separated by 153 nucleotides
malE and malK are separated by 364 nucleotides
malK and lamB are separated by 71 nucleotides
b4032: malG - maltose transporter subunit (NCBI), at 4,240,649 to 4,241,539
malG
b4033: malF - maltose transporter subunit (NCBI), at 4,241,554 to 4,243,098
malF
b4034: malE - maltose ABC transporter periplasmic protein (NCBI), at 4,243,252 to 4,244,442
malE
b4035: malK - fused maltose transport subunit, ATP-binding component of ABC superfamily/regulatory protein (NCBI), at 4,244,807 to 4,245,922
malK
b4036: lamB - maltoporin precursor (NCBI), at 4,245,994 to 4,247,334
lamB
Group
Condition
b4032
b4033
b4034
b4035
b4036
malG
malF
malE
malK
lamB
carbon source
D-Maltose (C)
-2.7
-3.2
-2.9
-2.6
-0.3
carbon source
D-Maltose (C)
-2.6
-2.8
-2.7
-3.1
-0.2
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-1.3
-1.0
-0.4
-1.0
+0.4
stress
Aluminum chloride 5 mM
-0.1
-0.6
-0.1
-1.7
-0.7
stress
LB Aerobic with Ramoplanin 0.000125 mM
-1.0
-0.5
-0.6
-1.4
+0.2
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-1.0
-0.8
-0.5
-1.1
+0.3
phage
Δdbn_10
-0.4
-0.2
-0.6
-1.5
-0.5
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.1
-0.5
-0.4
-1.3
-0.3
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.4
-0.9
-0.5
-0.5
-0.1
stress
2,4-Dinitrophenol 2 mM
-0.0
+0.1
+0.0
-0.8
-1.6
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.3
-0.4
-0.0
-1.6
+0.0
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.5
-0.6
-0.2
-1.2
+0.3
dart
Outgrowth from conjugation with pRH23 in LB+Gent50+Kan100
-0.4
-0.5
-0.4
-0.3
-0.3
stress
2,4-Dinitrophenol 2 mM
+0.1
+0.2
+0.3
-0.3
-1.9
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.4
-0.5
+0.1
-1.3
-0.1
phage
T7_phage 0.00015625 MOI TC2
+0.0
+0.4
+0.1
-1.9
+0.0
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.3
-0.3
+0.5
-1.0
+0.3
stress
Benzalkonium Chloride 0.004 mg/ml
-0.5
-0.1
-0.6
-0.4
+0.7
phage
Papa dilution 10-1 cacl2 maltose
-0.4
-0.4
-0.5
-0.2
+0.6
motility
outer cut, LB soft agar motility assay
-0.6
-0.1
-0.4
-0.3
+0.6
phage
T7_phage 0.0015625 MOI TC1
-0.0
+0.6
+0.5
-1.3
-0.2
phage
T7_phage 0.00015625 MOI TC4
-0.5
+0.8
+0.6
-0.8
-0.3
motility
outer cut, LB soft agar motility assay
-1.0
-0.2
+0.1
+0.1
+1.2
stress
2-undecanol 5 vol%
+0.4
+0.6
+0.4
-0.4
-0.4
control
PBS_cage_1_mouse_1_Cecum_40 hr
+0.4
+0.3
-0.6
+0.5
+0.3
phage
Lambda_ci1857
-3.7
-3.5
-0.4
+2.7
+7.9
fr_100_e2
Fr_100_E2
+0.6
+0.7
+0.3
+1.5
+5.1
fr_1000_e3
Fr_1000_E3
+0.5
+0.5
+0.2
+2.1
+6.3
fr_1000_f3
Fr_1000_F3
+0.7
+0.5
+0.3
+2.2
+6.5
fr_100_f2
Fr_100_F2
+1.4
+1.3
+0.9
+2.6
+7.7
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