Fitness for 5 genes in Escherichia coli BW25113

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500 ntmalG and malF are separated by 14 nucleotidesmalF and malE are separated by 153 nucleotidesmalE and malK are separated by 364 nucleotidesmalK and lamB are separated by 71 nucleotides b4032: malG - maltose transporter subunit (NCBI), at 4,240,649 to 4,241,539 malG b4033: malF - maltose transporter subunit (NCBI), at 4,241,554 to 4,243,098 malF b4034: malE - maltose ABC transporter periplasmic protein (NCBI), at 4,243,252 to 4,244,442 malE b4035: malK - fused maltose transport subunit, ATP-binding component of ABC superfamily/regulatory protein (NCBI), at 4,244,807 to 4,245,922 malK b4036: lamB - maltoporin precursor (NCBI), at 4,245,994 to 4,247,334 lamB
Group Condition b4032 b4033 b4034 b4035 b4036
malG malF malE malK lamB
carbon source D-Maltose (C) -2.7 -3.2 -2.9 -2.6 -0.3
carbon source D-Maltose (C) -2.6 -2.8 -2.7 -3.1 -0.2
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -1.3 -1.0 -0.4 -1.0 +0.4
stress Aluminum chloride 5 mM -0.1 -0.6 -0.1 -1.7 -0.7
stress LB Aerobic with Ramoplanin 0.000125 mM -1.0 -0.5 -0.6 -1.4 +0.2
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -1.0 -0.8 -0.5 -1.1 +0.3
phage Δdbn_10 -0.4 -0.2 -0.6 -1.5 -0.5
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.1 -0.5 -0.4 -1.3 -0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.4 -0.9 -0.5 -0.5 -0.1
stress 2,4-Dinitrophenol 2 mM -0.0 +0.1 +0.0 -0.8 -1.6
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.3 -0.4 -0.0 -1.6 +0.0
stress LB Aerobic with Ramoplanin 0.000125 mM -0.5 -0.6 -0.2 -1.2 +0.3
dart Outgrowth from conjugation with pRH23 in LB+Gent50+Kan100 -0.4 -0.5 -0.4 -0.3 -0.3
stress 2,4-Dinitrophenol 2 mM +0.1 +0.2 +0.3 -0.3 -1.9
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.4 -0.5 +0.1 -1.3 -0.1
phage T7_phage 0.00015625 MOI TC2 +0.0 +0.4 +0.1 -1.9 +0.0
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.3 -0.3 +0.5 -1.0 +0.3
stress Benzalkonium Chloride 0.004 mg/ml -0.5 -0.1 -0.6 -0.4 +0.7
phage Papa dilution 10-1 cacl2 maltose -0.4 -0.4 -0.5 -0.2 +0.6
motility outer cut, LB soft agar motility assay -0.6 -0.1 -0.4 -0.3 +0.6
phage T7_phage 0.0015625 MOI TC1 -0.0 +0.6 +0.5 -1.3 -0.2
phage T7_phage 0.00015625 MOI TC4 -0.5 +0.8 +0.6 -0.8 -0.3
motility outer cut, LB soft agar motility assay -1.0 -0.2 +0.1 +0.1 +1.2
stress 2-undecanol 5 vol% +0.4 +0.6 +0.4 -0.4 -0.4
control PBS_cage_1_mouse_1_Cecum_40 hr +0.4 +0.3 -0.6 +0.5 +0.3
phage Lambda_ci1857 -3.7 -3.5 -0.4 +2.7 +7.9
fr_100_e2 Fr_100_E2 +0.6 +0.7 +0.3 +1.5 +5.1
fr_1000_e3 Fr_1000_E3 +0.5 +0.5 +0.2 +2.1 +6.3
fr_1000_f3 Fr_1000_F3 +0.7 +0.5 +0.3 +2.2 +6.5
fr_100_f2 Fr_100_F2 +1.4 +1.3 +0.9 +2.6 +7.7
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