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Protein
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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
aceB and aceA are separated by 29 nucleotides
aceA and aceK are separated by 182 nucleotides
aceK and arpA overlap by 32 nucleotides
arpA and iclR are separated by 316 nucleotides
b4014: aceB - malate synthase (NCBI), at 4,213,501 to 4,215,102
aceB
b4015: aceA - isocitrate lyase (NCBI), at 4,215,132 to 4,216,436
aceA
b4016: aceK - bifunctional isocitrate dehydrogenase kinase/phosphatase protein (NCBI), at 4,216,619 to 4,218,355
aceK
b4017: arpA - regulator of acetyl CoA synthetase (NCBI), at 4,218,324 to 4,220,510
arpA
b4018: iclR - repressor of aceBA operon (VIMSS), at 4,220,827 to 4,221,651
iclR
Group
Condition
b4014
b4015
b4016
b4017
b4018
aceB
aceA
aceK
arpA
iclR
carbon source
acetate (C)
-0.9
-4.7
+1.0
-0.3
-1.1
carbon source
Acetate (C)
-2.3
-4.5
+1.2
+0.1
-0.4
carbon source
acetate (C)
-0.9
-4.6
+1.5
-0.0
-1.0
carbon source
Acetate (C)
-1.5
-4.3
+0.7
+0.1
-0.1
M9 Acetate 0.5% aerobic
-0.8
-3.2
+0.3
-0.5
+0.0
M9 Acetate 0.5% aerobic
-0.6
-2.7
+0.4
+0.2
-0.3
carbon source
Ying_Nucleobase14 rep B; time point 3
+0.2
-0.6
-0.4
+0.4
-1.7
carbon source
Ying_sugar7 rep B; time point 3
+0.1
-0.3
-0.4
+0.4
-1.8
carbon source
succinate (C)
+0.7
+1.0
+0.4
-0.7
-3.0
carbon source
Uridine 0.4095 mM (C)
+0.3
-0.0
+0.4
+0.0
-2.3
carbon source
D-Xylose (C)
+0.5
+0.5
+0.3
-0.2
-2.6
carbon source
Guanosine 0.35306 mM (C)
-0.4
-0.6
-0.4
+0.9
-1.1
carbon source
D-Xylose (C)
+0.5
+0.4
+0.1
-0.4
-2.1
carbon source
D-Trehalose (C)
+0.4
+0.1
+0.4
-0.4
-1.9
carbon source
L-Malic (C)
+1.0
+1.4
+0.5
-0.8
-3.4
carbon source
D-Glucosamine Hydrochloride (C)
+1.1
+1.0
+0.8
-0.1
-3.8
carbon source
D-Glucosamine Hydrochloride (C)
+1.1
+0.8
+0.6
-0.2
-3.4
carbon source
carbon source D-Glucose 5mM
+0.8
-0.7
-0.2
+0.5
-1.3
carbon source
D-Trehalose (C)
+0.5
+0.4
+0.3
-0.1
-2.2
carbon source
succinate (C)
+1.1
+1.3
+0.4
-0.3
-3.3
carbon source
L-Malic (C)
+1.0
+1.3
+0.7
-0.2
-3.3
carbon source
L-Fucose (C)
+0.6
+0.6
+0.5
-0.1
-1.9
carbon source
D-Ribose (C)
+0.7
+0.9
+0.3
-0.3
-2.1
carbon source
carbon source D-Mannose 0.5mM
+0.4
+0.2
+0.1
+0.7
-1.5
carbon source
D-Ribose (C)
+1.1
+0.7
+0.3
-0.3
-1.6
carbon source
carbon source D-Mannose 1mM
+0.6
+0.6
-0.3
+0.8
-1.0
carbon source
a-Ketoglutaric (C)
+0.8
+1.1
+0.2
-0.3
-0.8
carbon source
Glycolic Acid (C)
+2.9
+1.8
-0.0
-0.7
-1.4
carbon source
Glycolic Acid (C)
+3.0
+1.8
+0.1
-0.5
-1.5
phage
Papa dilution 10-1 cacl2 maltose
+0.1
+0.2
+0.5
+1.9
+0.6
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