Fitness for 5 genes in Escherichia coli BW25113

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500 ntaceB and aceA are separated by 29 nucleotidesaceA and aceK are separated by 182 nucleotidesaceK and arpA overlap by 32 nucleotidesarpA and iclR are separated by 316 nucleotides b4014: aceB - malate synthase (NCBI), at 4,213,501 to 4,215,102 aceB b4015: aceA - isocitrate lyase (NCBI), at 4,215,132 to 4,216,436 aceA b4016: aceK - bifunctional isocitrate dehydrogenase kinase/phosphatase protein (NCBI), at 4,216,619 to 4,218,355 aceK b4017: arpA - regulator of acetyl CoA synthetase (NCBI), at 4,218,324 to 4,220,510 arpA b4018: iclR - repressor of aceBA operon (VIMSS), at 4,220,827 to 4,221,651 iclR
Group Condition b4014 b4015 b4016 b4017 b4018
aceB aceA aceK arpA iclR
carbon source acetate (C) -0.9 -4.7 +1.0 -0.3 -1.1
carbon source Acetate (C) -2.3 -4.5 +1.2 +0.1 -0.4
carbon source acetate (C) -0.9 -4.6 +1.5 -0.0 -1.0
carbon source Acetate (C) -1.5 -4.3 +0.7 +0.1 -0.1
M9 Acetate 0.5% aerobic -0.8 -3.2 +0.3 -0.5 +0.0
M9 Acetate 0.5% aerobic -0.6 -2.7 +0.4 +0.2 -0.3
carbon source Ying_Nucleobase14 rep B; time point 3 +0.2 -0.6 -0.4 +0.4 -1.7
carbon source Ying_sugar7 rep B; time point 3 +0.1 -0.3 -0.4 +0.4 -1.8
carbon source succinate (C) +0.7 +1.0 +0.4 -0.7 -3.0
carbon source Uridine 0.4095 mM (C) +0.3 -0.0 +0.4 +0.0 -2.3
carbon source D-Xylose (C) +0.5 +0.5 +0.3 -0.2 -2.6
carbon source Guanosine 0.35306 mM (C) -0.4 -0.6 -0.4 +0.9 -1.1
carbon source D-Xylose (C) +0.5 +0.4 +0.1 -0.4 -2.1
carbon source D-Trehalose (C) +0.4 +0.1 +0.4 -0.4 -1.9
carbon source L-Malic (C) +1.0 +1.4 +0.5 -0.8 -3.4
carbon source D-Glucosamine Hydrochloride (C) +1.1 +1.0 +0.8 -0.1 -3.8
carbon source D-Glucosamine Hydrochloride (C) +1.1 +0.8 +0.6 -0.2 -3.4
carbon source carbon source D-Glucose 5mM +0.8 -0.7 -0.2 +0.5 -1.3
carbon source D-Trehalose (C) +0.5 +0.4 +0.3 -0.1 -2.2
carbon source succinate (C) +1.1 +1.3 +0.4 -0.3 -3.3
carbon source L-Malic (C) +1.0 +1.3 +0.7 -0.2 -3.3
carbon source L-Fucose (C) +0.6 +0.6 +0.5 -0.1 -1.9
carbon source D-Ribose (C) +0.7 +0.9 +0.3 -0.3 -2.1
carbon source carbon source D-Mannose 0.5mM +0.4 +0.2 +0.1 +0.7 -1.5
carbon source D-Ribose (C) +1.1 +0.7 +0.3 -0.3 -1.6
carbon source carbon source D-Mannose 1mM +0.6 +0.6 -0.3 +0.8 -1.0
carbon source a-Ketoglutaric (C) +0.8 +1.1 +0.2 -0.3 -0.8
carbon source Glycolic Acid (C) +2.9 +1.8 -0.0 -0.7 -1.4
carbon source Glycolic Acid (C) +3.0 +1.8 +0.1 -0.5 -1.5
phage Papa dilution 10-1 cacl2 maltose +0.1 +0.2 +0.5 +1.9 +0.6
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