Fitness for 5 genes in Escherichia coli BW25113

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500 ntmetA and aceB are separated by 268 nucleotidesaceB and aceA are separated by 29 nucleotidesaceA and aceK are separated by 182 nucleotidesaceK and arpA overlap by 32 nucleotides b4013: metA - homoserine O-succinyltransferase (NCBI), at 4,212,303 to 4,213,232 metA b4014: aceB - malate synthase (NCBI), at 4,213,501 to 4,215,102 aceB b4015: aceA - isocitrate lyase (NCBI), at 4,215,132 to 4,216,436 aceA b4016: aceK - bifunctional isocitrate dehydrogenase kinase/phosphatase protein (NCBI), at 4,216,619 to 4,218,355 aceK b4017: arpA - regulator of acetyl CoA synthetase (NCBI), at 4,218,324 to 4,220,510 arpA
Group Condition b4013 b4014 b4015 b4016 b4017
metA aceB aceA aceK arpA
carbon source Ying_sugar7 rep A; time point 3 -9.4 -0.0 -0.4 -0.2 +0.2
carbon source Ying_sugar7 rep C; time point 2 -9.6 +0.1 +0.0 -0.1 -0.1
carbon source Ying_OrganicAcid7 rep B; time point 3 -9.6 -0.0 +0.1 -0.3 +0.1
carbon source Ying_sugar7 rep B; time point 3 -9.5 +0.1 -0.3 -0.4 +0.4
carbon source Ying_sugar7 rep A; time point 2 -9.6 +0.2 -0.2 -0.1 +0.1
carbon source Ying_Nucleobase14 rep A; time point 3 -8.7 +0.2 -0.5 -0.3 +0.2
carbon source Ying_Nucleobase14 rep C; time point 3 -8.6 +0.1 -0.3 -0.5 +0.3
carbon source carbon source D-Mannose 1mM -9.0 +0.1 -0.2 +0.1 +0.0
carbon source Ying_sugar7 rep C; time point 3 -9.3 +0.3 -0.1 -0.3 +0.6
carbon source Ying_sugar7 rep B; time point 2 -8.8 +0.3 -0.0 -0.1 +0.0
carbon source Ying_OrganicAcid7 rep A; time point 3 -8.6 +0.2 -0.0 -0.0 +0.2
carbon source Ying_Nucleobase14 rep B; time point 3 -7.7 +0.2 -0.6 -0.4 +0.4
carbon source Ying_OrganicAcid7 rep C; time point 3 -8.0 +0.1 +0.0 -0.1 -0.0
carbon source carbon source D-Mannose 0.5mM -8.7 +0.3 -0.1 -0.0 +0.6
carbon source Ying_Nucleobase14 rep B; time point 2 -7.9 +0.3 -0.4 -0.2 +0.3
carbon source carbon source D-Mannose 1mM -8.8 +0.6 +0.6 -0.3 +0.8
carbon source carbon source D-Glucose 0.25mM -5.1 -0.2 -1.0 -0.1 -0.4
carbon source acetate (C) -2.0 -0.9 -4.6 +1.5 -0.0
carbon source Ying_Nucleobase14 rep A; time point 2 -6.4 +0.4 -0.1 -0.1 +0.2
carbon source Ying_OrganicAcid7 rep A; time point 2 -6.2 +0.1 -0.1 +0.1 +0.2
carbon source Acetate (C) -0.2 -2.3 -4.5 +1.2 +0.1
carbon source acetate (C) -0.6 -0.9 -4.7 +1.0 -0.3
carbon source Acetate (C) -0.1 -1.5 -4.3 +0.7 +0.1
carbon source carbon source D-Glucose 5mM -4.4 +0.8 -0.7 -0.2 +0.5
carbon source succinate (C) -4.9 +1.1 +1.3 +0.4 -0.3
carbon source L-Malic (C) -3.5 +1.0 +1.4 +0.5 -0.8
carbon source L-Malic (C) -3.9 +1.0 +1.3 +0.7 -0.2
carbon source D-Glucosamine Hydrochloride (C) -3.7 +1.1 +1.0 +0.8 -0.1
carbon source Glycolic Acid (C) -3.7 +3.0 +1.8 +0.1 -0.5
carbon source Glycolic Acid (C) -3.5 +2.9 +1.8 -0.0 -0.7
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