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Fitness for 5 genes in
Escherichia coli BW25113
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
yijD and trmA are separated by 39 nucleotides
trmA and btuB are separated by 368 nucleotides
btuB and murI overlap by 56 nucleotides
murI and rrsB are separated by 373 nucleotides
b3964: yijD - conserved inner membrane protein (NCBI), at 4,159,794 to 4,160,153
yijD
b3965: trmA - tRNA (uracil-5-)-methyltransferase (NCBI), at 4,160,193 to 4,161,293
trmA
b3966: btuB - vitamin B12/cobalamin outer membrane transporter (NCBI), at 4,161,662 to 4,163,506
btuB
b3967: murI - glutamate racemase, required for biosynthesis of D-glutamate and peptidoglycan (VIMSS), at 4,163,451 to 4,164,308
murI
b3968: rrsB - 16S ribosomal RNA (NCBI), at 4,164,682 to 4,166,223
rrsB
Group
Condition
b3964
b3965
b3966
b3967
b3968
yijD
trmA
btuB
murI
rrsB
stress
Benzalkonium Chloride 0.004 mg/ml
-0.8
-0.9
-0.7
N.D.
N.D.
carbon source
Ying_OrganicAcid7 rep A; time point 3
-0.1
-0.8
-1.0
N.D.
N.D.
carbon source
Ying_sugar7 rep C; time point 3
-0.2
-0.8
-0.8
N.D.
N.D.
carbon source
Ying_OrganicAcid7 rep C; time point 3
+0.0
-0.7
-1.1
N.D.
N.D.
motility
outer cut, LB soft agar motility assay
+0.0
-0.4
-1.4
N.D.
N.D.
carbon source
carbon source D-Glucose 0.25mM
-0.1
-1.2
-0.4
N.D.
N.D.
carbon source
Ying_OrganicAcid7 rep B; time point 3
+0.2
-0.7
-1.0
N.D.
N.D.
motility
outer cut, LB soft agar motility assay
+0.0
-0.0
-1.5
N.D.
N.D.
carbon source
Ying_OrganicAcid7 rep B; time point 2
-0.1
-0.7
-0.7
N.D.
N.D.
carbon source
Ying_sugar7 rep B; time point 3
-0.0
-0.6
-0.8
N.D.
N.D.
carbon source
carbon source D-Mannose 1mM
-0.4
-0.4
-0.6
N.D.
N.D.
carbon source
Ying_Nucleobase14 rep A; time point 3
-0.2
-0.9
-0.3
N.D.
N.D.
motility
inner cut, LB soft agar motility assay
-0.4
-0.5
-0.5
N.D.
N.D.
carbon source
Ying_sugar7 rep B; time point 2
-0.1
-0.7
-0.6
N.D.
N.D.
carbon source
D-Serine (C)
+0.3
-0.3
-1.3
N.D.
N.D.
carbon source
D-Serine (C)
+0.3
-0.2
-1.3
N.D.
N.D.
carbon source
Ying_OrganicAcid7 rep C; time point 2
+0.1
-0.5
-0.7
N.D.
N.D.
carbon source
Ying_OrganicAcid7 rep A; time point 2
+0.3
-0.7
-0.7
N.D.
N.D.
carbon source
Ying_Nucleobase14 rep B; time point 3
+0.2
-0.9
-0.3
N.D.
N.D.
stress
Trimethoprim 0.00125 mM
-1.1
+0.5
-0.4
N.D.
N.D.
stress
Trimethoprim 0.0025 mM
-1.0
+0.4
-0.4
N.D.
N.D.
phage
RB68
+0.3
-0.7
-0.5
N.D.
N.D.
phage
RB68
+0.4
-0.4
-0.6
N.D.
N.D.
phage
Ste41
+0.5
-0.5
-0.5
N.D.
N.D.
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+0.8
-0.7
-0.4
N.D.
N.D.
stress
2-Furfuraldehyde 0.25 vol%
-0.0
+0.7
-0.9
N.D.
N.D.
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.8
+0.5
+0.2
N.D.
N.D.
stress
Cephalothin 0.01 mg/ml
+0.7
-0.6
-0.2
N.D.
N.D.
stress
LB Aerobic with Irgasan 0.0000003125 mM
-0.2
+0.5
+0.7
N.D.
N.D.
phage
T7_phage 0.00015625 MOI TC4
+0.8
+0.6
+0.4
N.D.
N.D.
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