Fitness for 5 genes in Escherichia coli BW25113

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500 ntyijD and trmA are separated by 39 nucleotidestrmA and btuB are separated by 368 nucleotidesbtuB and murI overlap by 56 nucleotidesmurI and rrsB are separated by 373 nucleotides b3964: yijD - conserved inner membrane protein (NCBI), at 4,159,794 to 4,160,153 yijD b3965: trmA - tRNA (uracil-5-)-methyltransferase (NCBI), at 4,160,193 to 4,161,293 trmA b3966: btuB - vitamin B12/cobalamin outer membrane transporter (NCBI), at 4,161,662 to 4,163,506 btuB b3967: murI - glutamate racemase, required for biosynthesis of D-glutamate and peptidoglycan (VIMSS), at 4,163,451 to 4,164,308 murI b3968: rrsB - 16S ribosomal RNA (NCBI), at 4,164,682 to 4,166,223 rrsB
Group Condition b3964 b3965 b3966 b3967 b3968
yijD trmA btuB murI rrsB
stress Benzalkonium Chloride 0.004 mg/ml -0.8 -0.9 -0.7 N.D. N.D.
carbon source Ying_OrganicAcid7 rep A; time point 3 -0.1 -0.8 -1.0 N.D. N.D.
carbon source Ying_sugar7 rep C; time point 3 -0.2 -0.8 -0.8 N.D. N.D.
carbon source Ying_OrganicAcid7 rep C; time point 3 +0.0 -0.7 -1.1 N.D. N.D.
motility outer cut, LB soft agar motility assay +0.0 -0.4 -1.4 N.D. N.D.
carbon source carbon source D-Glucose 0.25mM -0.1 -1.2 -0.4 N.D. N.D.
carbon source Ying_OrganicAcid7 rep B; time point 3 +0.2 -0.7 -1.0 N.D. N.D.
motility outer cut, LB soft agar motility assay +0.0 -0.0 -1.5 N.D. N.D.
carbon source Ying_OrganicAcid7 rep B; time point 2 -0.1 -0.7 -0.7 N.D. N.D.
carbon source Ying_sugar7 rep B; time point 3 -0.0 -0.6 -0.8 N.D. N.D.
carbon source carbon source D-Mannose 1mM -0.4 -0.4 -0.6 N.D. N.D.
carbon source Ying_Nucleobase14 rep A; time point 3 -0.2 -0.9 -0.3 N.D. N.D.
motility inner cut, LB soft agar motility assay -0.4 -0.5 -0.5 N.D. N.D.
carbon source Ying_sugar7 rep B; time point 2 -0.1 -0.7 -0.6 N.D. N.D.
carbon source D-Serine (C) +0.3 -0.3 -1.3 N.D. N.D.
carbon source D-Serine (C) +0.3 -0.2 -1.3 N.D. N.D.
carbon source Ying_OrganicAcid7 rep C; time point 2 +0.1 -0.5 -0.7 N.D. N.D.
carbon source Ying_OrganicAcid7 rep A; time point 2 +0.3 -0.7 -0.7 N.D. N.D.
carbon source Ying_Nucleobase14 rep B; time point 3 +0.2 -0.9 -0.3 N.D. N.D.
stress Trimethoprim 0.00125 mM -1.1 +0.5 -0.4 N.D. N.D.
stress Trimethoprim 0.0025 mM -1.0 +0.4 -0.4 N.D. N.D.
phage RB68 +0.3 -0.7 -0.5 N.D. N.D.
phage RB68 +0.4 -0.4 -0.6 N.D. N.D.
phage Ste41 +0.5 -0.5 -0.5 N.D. N.D.
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.8 -0.7 -0.4 N.D. N.D.
stress 2-Furfuraldehyde 0.25 vol% -0.0 +0.7 -0.9 N.D. N.D.
stress LB Aerobic with Ramoplanin 0.000125 mM -0.8 +0.5 +0.2 N.D. N.D.
stress Cephalothin 0.01 mg/ml +0.7 -0.6 -0.2 N.D. N.D.
stress LB Aerobic with Irgasan 0.0000003125 mM -0.2 +0.5 +0.7 N.D. N.D.
phage T7_phage 0.00015625 MOI TC4 +0.8 +0.6 +0.4 N.D. N.D.
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