Fitness for 5 genes in Escherichia coli BW25113

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500 ntoxyR and udhA overlap by 18 nucleotidesudhA and fabR are separated by 333 nucleotidesfabR and yijD overlap by 1 nucleotidesyijD and trmA are separated by 39 nucleotides b3961: oxyR - DNA-binding transcriptional dual regulator (NCBI), at 4,156,513 to 4,157,430 oxyR b3962: udhA - putative oxidoreductase (VIMSS), at 4,157,413 to 4,158,813 udhA b3963: fabR - DNA-binding transcriptional repressor (RefSeq), at 4,159,147 to 4,159,794 fabR b3964: yijD - conserved inner membrane protein (NCBI), at 4,159,794 to 4,160,153 yijD b3965: trmA - tRNA (uracil-5-)-methyltransferase (NCBI), at 4,160,193 to 4,161,293 trmA
Group Condition b3961 b3962 b3963 b3964 b3965
oxyR udhA fabR yijD trmA
control 20 hr rep1_No_BD100_grewON -8.4 -0.3 -0.1 -0.4 -0.3
nophagecontrol NoPhageControl -8.2 -0.7 +0.0 -0.2 +0.0
phage P2_phage_0.5625_MOI -8.0 -0.7 -0.4 +0.2 -0.1
control 20 hr rep2_No_BD100_grewON -7.8 -0.2 +0.0 -0.5 -0.2
carbon source acetate (C) -3.7 -4.3 +0.3 +0.2 -0.6
stress undecene 5 vol% -5.5 -0.8 -1.0 -0.4 +0.5
stress undecene 5 vol% -5.4 -1.0 -1.2 -0.2 +0.5
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -6.0 +0.1 -0.3 -0.4 -0.4
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -5.4 -0.5 -0.6 -0.0 -0.2
stress undecene 2.5 vol% -4.7 -0.7 -1.5 -0.2 +0.4
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -5.9 -0.2 -0.1 -0.0 -0.1
stress undecene 2.5 vol% -4.3 -0.9 -1.1 -0.2 +0.3
stress LB Aerobic with Nitrofurantoin 0.00003125 mM -5.0 -0.2 -0.3 -0.2 -0.3
carbon source acetate (C) -1.8 -3.9 +0.3 +0.2 -0.4
stress Spectinomycin 0.0125 mg/ml -3.2 -2.4 -0.1 +0.5 +0.0
stress methylglyoxal 0.008 vol% -5.4 +0.8 -0.1 +0.1 -0.1
stress 1-ethyl-3-methylimidazolium chloride 80 mM -4.3 +1.0 -0.3 +0.1 -0.4
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.5 +0.7 -4.5 +0.5 +0.5
plasmid transformation mariner vector transformation; liquid outgrowth assay +5.8 +0.7 -0.5 +0.4 -0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay +6.7 +0.3 -0.5 +0.2 -0.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay +6.4 +0.3 -0.5 +0.4 -0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay +6.7 +0.6 -0.5 +0.5 -0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay +7.3 +0.2 -0.4 +0.2 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +7.1 +0.8 -0.4 +0.0 -0.2
plasmid transformation vcDART vector transformation; agar assay +7.1 -0.3 -0.0 +0.4 +0.3
plasmid transformation mariner vector transformation; agar assay +7.1 -0.4 +0.2 +0.2 +0.5
plasmid transformation mariner vector transformation; agar assay +7.2 -0.4 +0.1 +0.3 +0.5
plasmid transformation vcDART vector transformation; agar assay +7.4 -0.4 +0.1 +0.4 +0.4
plasmid transformation vcDART vector transformation; agar assay +7.3 -0.3 +0.2 +0.4 +0.4
plasmid transformation mariner vector transformation; agar assay +7.3 -0.4 +0.2 +0.4 +0.5
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