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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
oxyR and udhA overlap by 18 nucleotides
udhA and fabR are separated by 333 nucleotides
fabR and yijD overlap by 1 nucleotides
yijD and trmA are separated by 39 nucleotides
b3961: oxyR - DNA-binding transcriptional dual regulator (NCBI), at 4,156,513 to 4,157,430
oxyR
b3962: udhA - putative oxidoreductase (VIMSS), at 4,157,413 to 4,158,813
udhA
b3963: fabR - DNA-binding transcriptional repressor (RefSeq), at 4,159,147 to 4,159,794
fabR
b3964: yijD - conserved inner membrane protein (NCBI), at 4,159,794 to 4,160,153
yijD
b3965: trmA - tRNA (uracil-5-)-methyltransferase (NCBI), at 4,160,193 to 4,161,293
trmA
Group
Condition
b3961
b3962
b3963
b3964
b3965
oxyR
udhA
fabR
yijD
trmA
control
20 hr rep1_No_BD100_grewON
-8.4
-0.3
-0.1
-0.4
-0.3
nophagecontrol
NoPhageControl
-8.2
-0.7
+0.0
-0.2
+0.0
phage
P2_phage_0.5625_MOI
-8.0
-0.7
-0.4
+0.2
-0.1
control
20 hr rep2_No_BD100_grewON
-7.8
-0.2
+0.0
-0.5
-0.2
carbon source
acetate (C)
-3.7
-4.3
+0.3
+0.2
-0.6
stress
undecene 5 vol%
-5.5
-0.8
-1.0
-0.4
+0.5
stress
undecene 5 vol%
-5.4
-1.0
-1.2
-0.2
+0.5
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-6.0
+0.1
-0.3
-0.4
-0.4
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-5.4
-0.5
-0.6
-0.0
-0.2
stress
undecene 2.5 vol%
-4.7
-0.7
-1.5
-0.2
+0.4
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-5.9
-0.2
-0.1
-0.0
-0.1
stress
undecene 2.5 vol%
-4.3
-0.9
-1.1
-0.2
+0.3
stress
LB Aerobic with Nitrofurantoin 0.00003125 mM
-5.0
-0.2
-0.3
-0.2
-0.3
carbon source
acetate (C)
-1.8
-3.9
+0.3
+0.2
-0.4
stress
Spectinomycin 0.0125 mg/ml
-3.2
-2.4
-0.1
+0.5
+0.0
stress
methylglyoxal 0.008 vol%
-5.4
+0.8
-0.1
+0.1
-0.1
stress
1-ethyl-3-methylimidazolium chloride 80 mM
-4.3
+1.0
-0.3
+0.1
-0.4
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.5
+0.7
-4.5
+0.5
+0.5
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+5.8
+0.7
-0.5
+0.4
-0.3
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+6.7
+0.3
-0.5
+0.2
-0.4
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+6.4
+0.3
-0.5
+0.4
-0.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+6.7
+0.6
-0.5
+0.5
-0.4
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+7.3
+0.2
-0.4
+0.2
-0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+7.1
+0.8
-0.4
+0.0
-0.2
plasmid transformation
vcDART vector transformation; agar assay
+7.1
-0.3
-0.0
+0.4
+0.3
plasmid transformation
mariner vector transformation; agar assay
+7.1
-0.4
+0.2
+0.2
+0.5
plasmid transformation
mariner vector transformation; agar assay
+7.2
-0.4
+0.1
+0.3
+0.5
plasmid transformation
vcDART vector transformation; agar assay
+7.4
-0.4
+0.1
+0.4
+0.4
plasmid transformation
vcDART vector transformation; agar assay
+7.3
-0.3
+0.2
+0.4
+0.4
plasmid transformation
mariner vector transformation; agar assay
+7.3
-0.4
+0.2
+0.4
+0.5
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