Fitness for 5 genes in Escherichia coli BW25113

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500 ntptsA and frwC are separated by 308 nucleotidesfrwC and frwB are separated by 14 nucleotidesfrwB and pflD are separated by 50 nucleotidespflD and pflC overlap by 35 nucleotides b3947: ptsA - fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component (NCBI), at 4,137,743 to 4,140,244 ptsA b3949: frwC - predicted enzyme IIC component of PTS (NCBI), at 4,140,553 to 4,141,632 frwC b3950: frwB - predicted enzyme IIB component of PTS (NCBI), at 4,141,647 to 4,141,967 frwB b3951: pflD - predicted formate acetyltransferase 2 (pyruvate formate lyase II) (NCBI), at 4,142,018 to 4,144,315 pflD b3952: pflC - probable pyruvate formate lyase activating enzyme 2 (VIMSS), at 4,144,281 to 4,145,159 pflC
Group Condition b3947 b3949 b3950 b3951 b3952
ptsA frwC frwB pflD pflC
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.2 -0.0 -2.0 -0.3 +0.2
carbon source carbon source D-Glucose 5mM -0.2 -0.2 -1.4 -0.0 -0.2
carbon source carbon source D-Glucose 0.25mM -0.5 +0.2 -1.9 +0.1 +0.2
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM -0.3 -0.1 -0.9 -0.2 +0.1
stress .5% DMSO -0.0 +0.0 -1.1 -0.2 -0.2
nitrogen source Gly-DL-Asp (N) -0.1 +0.0 -1.3 -0.0 -0.0
control PBS_cage_1_mice _Feces_40 hr -0.3 -0.1 -0.8 -0.2 +0.1
mixed community LB 0.1x with Mixed culture; Trichoderma virens G41 +0.1 -0.0 -1.0 -0.1 -0.4
stress Cisplatin 0.1 mg/ml +0.1 +0.0 -1.6 -0.2 +0.3
predation 6 hr rep1_HD100_10fold_dilution_grewON -0.3 -0.0 -1.2 +0.2 +0.1
carbon source carbon source D-Mannose 1mM -0.2 -0.2 -0.9 +0.0 +0.1
stress 1-ethyl-3-methylimidazolium acetate 30 mM +0.1 -0.1 -1.2 +0.0 +0.1
stress LB Aerobic with Nitrofurantoin 0.00003125 mM +0.3 -0.1 -1.3 +0.1 +0.2
control PBS_cage_1_mouse_2_Cecum_40 hr -0.3 -0.3 -0.4 -0.3 +0.4
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +0.0 +0.2 -1.2 -0.1 +0.2
stress Spectinomycin 0.0125 mg/ml +0.4 -0.5 -0.4 -0.0 -0.3
stress Cisplatin 0.1 mg/ml +0.3 -0.1 -0.9 +0.2 -0.4
stress 5-Hydroxymethylfurfural 20 mM +0.2 -0.1 -1.0 +0.0 +0.2
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.2 -0.1 -0.5 +0.2 -0.5
stress LB Aerobic with Doxycycline hyclate 0.0000015625 mM +0.1 +0.1 -1.2 +0.1 +0.2
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -0.4 -0.3 +0.7 -0.5 -0.1
phage T7_phage 0.00015625 MOI TC3 -0.2 +0.3 -0.4 +0.2 -0.3
phage T7_phage 0.0015625 MOI TC2 +0.2 +0.2 -0.9 -0.3 +0.7
phage Papa dilution 10-1 cacl2 maltose +0.3 -0.1 -0.7 +0.2 +0.3
phage Ste41 +0.4 +0.2 -0.6 -0.3 +0.3
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.3 +0.4 +0.2 +0.2 -0.6
stress Chlorite 0.4 mM +0.1 +0.0 +0.8 +0.1 -0.4
stress Cephalothin 0.01 mg/ml -0.0 -0.0 +1.5 +0.1 -0.3
phage Ste41 +0.4 +0.5 +0.3 -0.1 +0.4
stress 3-methyl-1-butanol 1.25 vol% -0.1 +0.2 +1.3 -0.2 +0.5
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