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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
ptsA and frwC are separated by 308 nucleotides
frwC and frwB are separated by 14 nucleotides
frwB and pflD are separated by 50 nucleotides
pflD and pflC overlap by 35 nucleotides
b3947: ptsA - fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component (NCBI), at 4,137,743 to 4,140,244
ptsA
b3949: frwC - predicted enzyme IIC component of PTS (NCBI), at 4,140,553 to 4,141,632
frwC
b3950: frwB - predicted enzyme IIB component of PTS (NCBI), at 4,141,647 to 4,141,967
frwB
b3951: pflD - predicted formate acetyltransferase 2 (pyruvate formate lyase II) (NCBI), at 4,142,018 to 4,144,315
pflD
b3952: pflC - probable pyruvate formate lyase activating enzyme 2 (VIMSS), at 4,144,281 to 4,145,159
pflC
Group
Condition
b3947
b3949
b3950
b3951
b3952
ptsA
frwC
frwB
pflD
pflC
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.2
-0.0
-2.0
-0.3
+0.2
carbon source
carbon source D-Glucose 5mM
-0.2
-0.2
-1.4
-0.0
-0.2
carbon source
carbon source D-Glucose 0.25mM
-0.5
+0.2
-1.9
+0.1
+0.2
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
-0.3
-0.1
-0.9
-0.2
+0.1
stress
.5% DMSO
-0.0
+0.0
-1.1
-0.2
-0.2
nitrogen source
Gly-DL-Asp (N)
-0.1
+0.0
-1.3
-0.0
-0.0
control
PBS_cage_1_mice _Feces_40 hr
-0.3
-0.1
-0.8
-0.2
+0.1
mixed community
LB 0.1x with Mixed culture; Trichoderma virens G41
+0.1
-0.0
-1.0
-0.1
-0.4
stress
Cisplatin 0.1 mg/ml
+0.1
+0.0
-1.6
-0.2
+0.3
predation
6 hr rep1_HD100_10fold_dilution_grewON
-0.3
-0.0
-1.2
+0.2
+0.1
carbon source
carbon source D-Mannose 1mM
-0.2
-0.2
-0.9
+0.0
+0.1
stress
1-ethyl-3-methylimidazolium acetate 30 mM
+0.1
-0.1
-1.2
+0.0
+0.1
stress
LB Aerobic with Nitrofurantoin 0.00003125 mM
+0.3
-0.1
-1.3
+0.1
+0.2
control
PBS_cage_1_mouse_2_Cecum_40 hr
-0.3
-0.3
-0.4
-0.3
+0.4
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+0.0
+0.2
-1.2
-0.1
+0.2
stress
Spectinomycin 0.0125 mg/ml
+0.4
-0.5
-0.4
-0.0
-0.3
stress
Cisplatin 0.1 mg/ml
+0.3
-0.1
-0.9
+0.2
-0.4
stress
5-Hydroxymethylfurfural 20 mM
+0.2
-0.1
-1.0
+0.0
+0.2
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+0.2
-0.1
-0.5
+0.2
-0.5
stress
LB Aerobic with Doxycycline hyclate 0.0000015625 mM
+0.1
+0.1
-1.2
+0.1
+0.2
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-0.4
-0.3
+0.7
-0.5
-0.1
phage
T7_phage 0.00015625 MOI TC3
-0.2
+0.3
-0.4
+0.2
-0.3
phage
T7_phage 0.0015625 MOI TC2
+0.2
+0.2
-0.9
-0.3
+0.7
phage
Papa dilution 10-1 cacl2 maltose
+0.3
-0.1
-0.7
+0.2
+0.3
phage
Ste41
+0.4
+0.2
-0.6
-0.3
+0.3
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.3
+0.4
+0.2
+0.2
-0.6
stress
Chlorite 0.4 mM
+0.1
+0.0
+0.8
+0.1
-0.4
stress
Cephalothin 0.01 mg/ml
-0.0
-0.0
+1.5
+0.1
-0.3
phage
Ste41
+0.4
+0.5
+0.3
-0.1
+0.4
stress
3-methyl-1-butanol 1.25 vol%
-0.1
+0.2
+1.3
-0.2
+0.5
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