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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
menA and hslU are separated by 66 nucleotides
hslU and hslV are separated by 9 nucleotides
hslV and ftsN are separated by 92 nucleotides
ftsN and cytR are separated by 91 nucleotides
b3930: menA - 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI), at 4,117,446 to 4,118,372
menA
b3931: hslU - ATP-dependent protease ATP-binding subunit (NCBI), at 4,118,439 to 4,119,770
hslU
b3932: hslV - ATP-dependent protease peptidase subunit (NCBI), at 4,119,780 to 4,120,310
hslV
b3933: ftsN - essential cell division protein (NCBI), at 4,120,403 to 4,121,362
ftsN
b3934: cytR - DNA-binding transcriptional dual regulator (NCBI), at 4,121,454 to 4,122,479
cytR
Group
Condition
b3930
b3931
b3932
b3933
b3934
menA
hslU
hslV
ftsN
cytR
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-1.2
-4.0
-4.3
-1.2
+0.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-1.4
-4.1
-3.9
-1.1
+0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-1.2
-4.1
-4.2
-1.3
+0.5
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.4
-3.8
-4.6
-1.1
-0.3
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
-3.0
-3.0
-3.1
-0.4
+0.1
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.4
-3.9
-4.0
-0.9
-0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.6
-3.0
-3.4
-1.2
+0.5
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
-2.5
-2.5
-2.8
+0.1
+0.2
stress
octanol 0.08 vol%
+0.4
-5.3
-2.6
+0.3
-0.1
stress
hexanol 0.31 vol%
+0.2
-4.3
-2.3
-0.6
-0.1
stress
LB Aerobic with Irgasan 0.0000003125 mM
+0.4
-3.6
-3.6
-0.2
+0.2
stress
LB Anaerobic with Nitrofurantoin 0.000015625 mM
-3.9
-1.2
-1.1
-0.8
+0.4
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.4
-2.2
-3.1
-0.9
+0.2
stress
Spectinomycin 0.0125 mg/ml
+0.2
-0.9
-1.2
-1.2
-3.0
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.6
-2.1
-2.3
-1.4
+0.3
carbon source
Glycolic Acid (C)
-2.7
-0.9
-0.8
+1.2
-2.4
motility
outer cut, LB soft agar motility assay
-2.3
+0.8
+0.4
-4.7
+0.3
stress
hexanol 0.31 vol%
+0.2
-2.9
-2.0
-0.7
-0.1
carbon source
Glycolic Acid (C)
-2.5
-0.9
-0.8
+1.1
-2.3
stress
1-ethyl-3-methylimidazolium chloride 80 mM
+0.3
-0.1
-0.2
-0.8
-4.5
stress
Trimethoprim 0.0025 mM
-0.5
-1.0
-1.1
+0.3
+2.9
carbon source
Inosine 0.3728 mM (C)
-0.0
+0.1
-0.2
+0.3
+7.2
carbon source
Guanosine 0.35306 mM (C)
+0.2
+0.0
+0.0
+0.3
+7.3
carbon source
Guanosine 0.35306 mM (C)
+0.2
+0.3
+0.1
+0.3
+7.0
carbon source
Inosine 0.3728 mM (C)
+0.1
-0.1
-0.1
+0.1
+8.0
carbon source
Uridine 0.4095 mM (C)
-0.0
+0.0
+0.1
+0.4
+7.8
carbon source
Uridine 0.4095 mM (C)
+0.1
-0.0
+0.1
+0.7
+7.7
carbon source
Uridine 0.4095 mM (C)
-0.1
-0.1
+0.1
+0.6
+8.2
carbon source
Inosine 0.3728 mM (C)
+0.4
-0.0
-0.2
+0.2
+9.2
carbon source
Guanosine 0.35306 mM (C)
+0.3
-0.0
+0.1
-0.4
+10.1
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