Fitness for 5 genes in Escherichia coli BW25113

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500 ntmenA and hslU are separated by 66 nucleotideshslU and hslV are separated by 9 nucleotideshslV and ftsN are separated by 92 nucleotidesftsN and cytR are separated by 91 nucleotides b3930: menA - 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI), at 4,117,446 to 4,118,372 menA b3931: hslU - ATP-dependent protease ATP-binding subunit (NCBI), at 4,118,439 to 4,119,770 hslU b3932: hslV - ATP-dependent protease peptidase subunit (NCBI), at 4,119,780 to 4,120,310 hslV b3933: ftsN - essential cell division protein (NCBI), at 4,120,403 to 4,121,362 ftsN b3934: cytR - DNA-binding transcriptional dual regulator (NCBI), at 4,121,454 to 4,122,479 cytR
Group Condition b3930 b3931 b3932 b3933 b3934
menA hslU hslV ftsN cytR
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.2 -4.0 -4.3 -1.2 +0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.4 -4.1 -3.9 -1.1 +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.2 -4.1 -4.2 -1.3 +0.5
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.4 -3.8 -4.6 -1.1 -0.3
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM -3.0 -3.0 -3.1 -0.4 +0.1
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.4 -3.9 -4.0 -0.9 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.6 -3.0 -3.4 -1.2 +0.5
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM -2.5 -2.5 -2.8 +0.1 +0.2
stress octanol 0.08 vol% +0.4 -5.3 -2.6 +0.3 -0.1
stress hexanol 0.31 vol% +0.2 -4.3 -2.3 -0.6 -0.1
stress LB Aerobic with Irgasan 0.0000003125 mM +0.4 -3.6 -3.6 -0.2 +0.2
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM -3.9 -1.2 -1.1 -0.8 +0.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.4 -2.2 -3.1 -0.9 +0.2
stress Spectinomycin 0.0125 mg/ml +0.2 -0.9 -1.2 -1.2 -3.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.6 -2.1 -2.3 -1.4 +0.3
carbon source Glycolic Acid (C) -2.7 -0.9 -0.8 +1.2 -2.4
motility outer cut, LB soft agar motility assay -2.3 +0.8 +0.4 -4.7 +0.3
stress hexanol 0.31 vol% +0.2 -2.9 -2.0 -0.7 -0.1
carbon source Glycolic Acid (C) -2.5 -0.9 -0.8 +1.1 -2.3
stress 1-ethyl-3-methylimidazolium chloride 80 mM +0.3 -0.1 -0.2 -0.8 -4.5
stress Trimethoprim 0.0025 mM -0.5 -1.0 -1.1 +0.3 +2.9
carbon source Inosine 0.3728 mM (C) -0.0 +0.1 -0.2 +0.3 +7.2
carbon source Guanosine 0.35306 mM (C) +0.2 +0.0 +0.0 +0.3 +7.3
carbon source Guanosine 0.35306 mM (C) +0.2 +0.3 +0.1 +0.3 +7.0
carbon source Inosine 0.3728 mM (C) +0.1 -0.1 -0.1 +0.1 +8.0
carbon source Uridine 0.4095 mM (C) -0.0 +0.0 +0.1 +0.4 +7.8
carbon source Uridine 0.4095 mM (C) +0.1 -0.0 +0.1 +0.7 +7.7
carbon source Uridine 0.4095 mM (C) -0.1 -0.1 +0.1 +0.6 +8.2
carbon source Inosine 0.3728 mM (C) +0.4 -0.0 -0.2 +0.2 +9.2
carbon source Guanosine 0.35306 mM (C) +0.3 -0.0 +0.1 -0.4 +10.1
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