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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
rraA and menA are separated by 92 nucleotides
menA and hslU are separated by 66 nucleotides
hslU and hslV are separated by 9 nucleotides
hslV and ftsN are separated by 92 nucleotides
b3929: rraA - ribonuclease activity regulator protein RraA (NCBI), at 4,116,868 to 4,117,353
rraA
b3930: menA - 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI), at 4,117,446 to 4,118,372
menA
b3931: hslU - ATP-dependent protease ATP-binding subunit (NCBI), at 4,118,439 to 4,119,770
hslU
b3932: hslV - ATP-dependent protease peptidase subunit (NCBI), at 4,119,780 to 4,120,310
hslV
b3933: ftsN - essential cell division protein (NCBI), at 4,120,403 to 4,121,362
ftsN
Group
Condition
b3929
b3930
b3931
b3932
b3933
rraA
menA
hslU
hslV
ftsN
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.0
-1.2
-4.0
-4.3
-1.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.2
-1.2
-4.1
-4.2
-1.3
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.1
-1.4
-4.1
-3.9
-1.1
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.0
-0.4
-3.8
-4.6
-1.1
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.1
-3.0
-3.0
-3.1
-0.4
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.3
-0.4
-3.9
-4.0
-0.9
stress
LB Aerobic with Irgasan 0.0000003125 mM
-1.2
+0.4
-3.6
-3.6
-0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.1
-0.6
-3.0
-3.4
-1.2
stress
octanol 0.08 vol%
-0.7
+0.4
-5.3
-2.6
+0.3
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.0
-2.5
-2.5
-2.8
+0.1
stress
hexanol 0.31 vol%
-0.1
+0.2
-4.3
-2.3
-0.6
stress
LB Anaerobic with Nitrofurantoin 0.000015625 mM
+0.1
-3.9
-1.2
-1.1
-0.8
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.1
-0.4
-2.2
-3.1
-0.9
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.3
-0.6
-2.1
-2.3
-1.4
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-0.3
-1.7
-0.4
-0.5
-2.7
motility
outer cut, LB soft agar motility assay
+0.2
-2.3
+0.8
+0.4
-4.7
stress
hexanol 0.31 vol%
-0.1
+0.2
-2.9
-2.0
-0.7
m9 anaerobic no stress control
M9 anaerobic no stress control
-0.1
-0.8
-1.7
-2.0
-0.7
mzaabcde
mzaABCDE
-0.7
-1.5
-0.4
-0.6
-1.9
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.1
-1.6
-1.0
-1.0
-1.5
stress
3-methyl-1-butanol 1.25 vol%
-0.7
+0.2
-1.6
-1.7
-1.4
stress
pinene 0.3125 vol%
-0.4
-0.8
-1.5
-1.6
-0.8
m9 anaerobic no stress control
M9 anaerobic no stress control
-0.1
-0.9
-1.7
-1.8
-0.5
phage
Phage_N4_cage_3_mouse_1_Cecum_40 hr
+0.0
-1.3
-0.5
-0.6
-2.7
stress
LB Aerobic with Hygromycin B 0.000015625 mM
+0.2
+0.1
-2.0
-2.2
-1.0
stress
Cephalothin 0.01 mg/ml
+0.2
-0.6
-1.0
-1.5
-1.8
carbon source
Glycolic Acid (C)
+1.9
-2.7
-0.9
-0.8
+1.2
carbon source
Glycolic Acid (C)
+1.9
-2.5
-0.9
-0.8
+1.1
protonated/deuterated
deuterated glucose in D2O
+4.0
-0.3
+0.2
+0.2
+0.3
stress
M9 with Methotrexate 0.06 mM 48 hr
+2.0
-0.2
-0.0
-0.1
+2.9
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