Fitness for 5 genes in Escherichia coli BW25113

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500 ntrraA and menA are separated by 92 nucleotidesmenA and hslU are separated by 66 nucleotideshslU and hslV are separated by 9 nucleotideshslV and ftsN are separated by 92 nucleotides b3929: rraA - ribonuclease activity regulator protein RraA (NCBI), at 4,116,868 to 4,117,353 rraA b3930: menA - 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI), at 4,117,446 to 4,118,372 menA b3931: hslU - ATP-dependent protease ATP-binding subunit (NCBI), at 4,118,439 to 4,119,770 hslU b3932: hslV - ATP-dependent protease peptidase subunit (NCBI), at 4,119,780 to 4,120,310 hslV b3933: ftsN - essential cell division protein (NCBI), at 4,120,403 to 4,121,362 ftsN
Group Condition b3929 b3930 b3931 b3932 b3933
rraA menA hslU hslV ftsN
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.0 -1.2 -4.0 -4.3 -1.2
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.2 -1.2 -4.1 -4.2 -1.3
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.1 -1.4 -4.1 -3.9 -1.1
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.0 -0.4 -3.8 -4.6 -1.1
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.1 -3.0 -3.0 -3.1 -0.4
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.3 -0.4 -3.9 -4.0 -0.9
stress LB Aerobic with Irgasan 0.0000003125 mM -1.2 +0.4 -3.6 -3.6 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.1 -0.6 -3.0 -3.4 -1.2
stress octanol 0.08 vol% -0.7 +0.4 -5.3 -2.6 +0.3
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.0 -2.5 -2.5 -2.8 +0.1
stress hexanol 0.31 vol% -0.1 +0.2 -4.3 -2.3 -0.6
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM +0.1 -3.9 -1.2 -1.1 -0.8
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.1 -0.4 -2.2 -3.1 -0.9
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.3 -0.6 -2.1 -2.3 -1.4
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.3 -1.7 -0.4 -0.5 -2.7
motility outer cut, LB soft agar motility assay +0.2 -2.3 +0.8 +0.4 -4.7
stress hexanol 0.31 vol% -0.1 +0.2 -2.9 -2.0 -0.7
m9 anaerobic no stress control M9 anaerobic no stress control -0.1 -0.8 -1.7 -2.0 -0.7
mzaabcde mzaABCDE -0.7 -1.5 -0.4 -0.6 -1.9
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.1 -1.6 -1.0 -1.0 -1.5
stress 3-methyl-1-butanol 1.25 vol% -0.7 +0.2 -1.6 -1.7 -1.4
stress pinene 0.3125 vol% -0.4 -0.8 -1.5 -1.6 -0.8
m9 anaerobic no stress control M9 anaerobic no stress control -0.1 -0.9 -1.7 -1.8 -0.5
phage Phage_N4_cage_3_mouse_1_Cecum_40 hr +0.0 -1.3 -0.5 -0.6 -2.7
stress LB Aerobic with Hygromycin B 0.000015625 mM +0.2 +0.1 -2.0 -2.2 -1.0
stress Cephalothin 0.01 mg/ml +0.2 -0.6 -1.0 -1.5 -1.8
carbon source Glycolic Acid (C) +1.9 -2.7 -0.9 -0.8 +1.2
carbon source Glycolic Acid (C) +1.9 -2.5 -0.9 -0.8 +1.1
protonated/deuterated deuterated glucose in D2O +4.0 -0.3 +0.2 +0.2 +0.3
stress M9 with Methotrexate 0.06 mM 48 hr +2.0 -0.2 -0.0 -0.1 +2.9
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