Fitness for 5 genes in Escherichia coli BW25113

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500 ntglpK and glpF are separated by 22 nucleotidesglpF and yiiU are separated by 424 nucleotidesyiiU and rraA are separated by 84 nucleotidesrraA and menA are separated by 92 nucleotides b3926: glpK - glycerol kinase (NCBI), at 4,113,737 to 4,115,245 glpK b3927: glpF - glycerol facilitator (NCBI), at 4,115,268 to 4,116,113 glpF b3928: yiiU - hypothetical protein (NCBI), at 4,116,538 to 4,116,783 yiiU b3929: rraA - ribonuclease activity regulator protein RraA (NCBI), at 4,116,868 to 4,117,353 rraA b3930: menA - 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI), at 4,117,446 to 4,118,372 menA
Group Condition b3926 b3927 b3928 b3929 b3930
glpK glpF yiiU rraA menA
stress LB Anaerobic with Cephalothin 0.0000078125 mM -1.2 -0.2 -1.8 -0.1 -1.6
mzaabcde mzaABCDE -0.2 -1.9 -0.2 -0.7 -1.5
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) -4.1 -1.9 +0.4 +1.4 -0.2
carbon source HMW_DNA_from_Lysinibacillus_FW301-JW8 40.7 mM (C) -3.1 -2.2 +0.3 +0.7 -0.0
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) -3.8 -2.1 -0.1 +1.8 -0.0
no stress control anaerobic M9 no stress control; Anaerobic 24 hr -0.8 -0.2 -0.4 +0.5 -3.2
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +0.8 +0.3 -3.2 -0.3 -1.7
carbon source HMW_DNA_from_Lysinibacillus_FW301-JW8 40.7 mM (C) -2.6 -1.8 +0.4 +0.2 -0.1
carbon source HMW_DNA_from_Pseudomonas_EB271-B4-1 46.9 ppm (C) -2.5 -1.7 -0.0 +0.6 -0.1
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) -3.3 -2.0 +0.3 +1.6 -0.1
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +0.7 +0.3 -3.0 -0.1 -1.4
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) -3.1 -1.6 +0.2 +1.5 -0.2
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) -3.0 -1.6 +0.2 +1.8 -0.3
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) -3.1 -1.9 +0.4 +1.7 -0.0
stress LB Aerobic with Ramoplanin 0.000125 mM +1.2 +0.7 -5.7 +0.0 +0.8
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM +0.9 +0.3 -0.2 +0.1 -3.9
stress LB Aerobic with Ramoplanin 0.000125 mM +1.0 +0.7 -4.6 -0.1 +0.7
carbon source Glycolic Acid (C) +0.2 +0.2 -0.1 +1.9 -2.5
carbon source Glycolic Acid (C) +0.7 +0.8 -0.6 +1.9 -2.7
carbon source a-Ketoglutaric (C) -1.2 -0.4 -0.0 +2.5 +0.2
carbon source acetate (C) -1.1 -0.4 +0.3 +2.7 -0.4
carbon source acetate (C) -0.9 -0.5 +0.6 +2.3 -0.3
carbon source a-Ketoglutaric (C) -1.0 -0.3 -0.2 +2.8 +0.1
stress M9 with Methotrexate 0.06 mM 48 hr -0.5 +0.1 -0.3 +3.7 -0.3
carbon source succinate (C) +1.4 +0.9 -0.7 +1.6 -0.5
protonated/deuterated deuterated glucose in D2O -0.1 -0.1 +0.4 +3.6 -0.3
protonated/deuterated deuterated glucose in D2O +0.0 -0.0 +0.1 +4.0 -0.3
carbon source Acetate (C) +0.9 +0.3 -0.0 +2.9 -0.2
carbon source Acetate (C) +0.9 +0.4 -0.1 +2.8 -0.1
stress Phosphomycin 0.001 mg/ml +5.5 +0.8 +0.1 -0.0 -0.1
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