Fitness for 5 genes in Escherichia coli BW25113

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500 ntyiiS and yiiT are separated by 26 nucleotidesyiiT and fpr are separated by 4 nucleotidesfpr and glpX are separated by 96 nucleotidesglpX and glpK are separated by 134 nucleotides b3922: yiiS - hypothetical protein (NCBI), at 4,110,990 to 4,111,289 yiiS b3923: yiiT - stress-induced protein (NCBI), at 4,111,316 to 4,111,744 yiiT b3924: fpr - ferredoxin-NADP reductase (NCBI), at 4,111,749 to 4,112,495 fpr b3925: glpX - fructose 1,6-bisphosphatase II (NCBI), at 4,112,592 to 4,113,602 glpX b3926: glpK - glycerol kinase (NCBI), at 4,113,737 to 4,115,245 glpK
Group Condition b3922 b3923 b3924 b3925 b3926
yiiS yiiT fpr glpX glpK
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) +0.0 +0.1 -0.1 -0.4 -4.1
stress M9 Aerobic Capecitabine 5000 uM +0.1 -0.1 -2.0 +0.0 -2.0
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) +0.2 +0.1 +0.0 -0.4 -3.8
stress M9 Aerobic Capecitabine 5000 uM +0.0 +0.0 -2.0 -0.0 -1.9
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) +0.0 +0.1 -0.3 -0.3 -3.1
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) +0.1 +0.1 +0.0 -0.3 -3.3
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) +0.1 +0.2 -0.0 -0.3 -3.0
carbon source HMW_DNA_from_Lysinibacillus_FW301-JW8 40.7 mM (C) +0.0 +0.1 -0.1 +0.0 -3.1
carbon source HMW_DNA_from_Pseudomonas_EB271-B4-1 46.9 ppm (C) -0.0 +0.0 -0.3 -0.2 -2.5
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) +0.1 +0.2 +0.1 -0.2 -3.1
stress M9 Anaerobic Capecitabine 5000 uM -0.1 -0.0 -1.1 -0.0 -1.6
m9 aerobic no stress control M9 aerobic no stress control +0.0 +0.0 -1.8 +0.0 -0.9
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr +0.0 -0.0 -1.5 -0.0 -1.1
m9 aerobic no stress control M9 aerobic no stress control -0.0 -0.0 -1.7 +0.0 -0.9
stress M9 Aerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM +0.0 -0.1 -1.6 +0.1 -1.0
stress M9 Aerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM +0.0 -0.0 -1.4 +0.0 -1.1
carbon source HMW_DNA_from_Lysinibacillus_FW301-JW8 40.7 mM (C) +0.1 +0.2 +0.2 -0.3 -2.6
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr +0.0 -0.0 -1.3 +0.0 -1.2
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM +0.1 -0.0 -2.8 -0.1 +0.5
stress M9 with Methotrexate 0.06 mM 48 hr +0.1 -0.0 -1.7 +0.0 -0.7
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM +0.2 +0.1 -2.8 -0.2 +0.6
stress M9 with Methotrexate 0.06 mM 24 hr +0.1 -0.0 -1.6 +0.0 -0.6
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr +0.1 -0.0 -1.6 +0.0 -0.6
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr +0.2 +0.1 -1.7 +0.0 -0.6
carbon source acetate (C) +0.3 +0.0 -0.8 -0.1 -1.1
stress 2,4-Dinitrophenol 2 mM +0.1 +0.1 +0.4 +0.1 -2.1
stress LB Aerobic with Nitrofurantoin 0.00003125 mM +0.0 -0.0 -1.4 +0.1 +0.7
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.2 +0.3 +0.4 -0.2 -1.2
carbon source succinate (C) +0.3 +0.1 -0.3 +0.2 +1.4
stress Phosphomycin 0.001 mg/ml +0.1 +0.2 -0.1 -0.1 +5.5
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