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Cofit
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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
cpxR and cpxP are separated by 149 nucleotides
cpxP and fieF are separated by 148 nucleotides
fieF and pfkA are separated by 180 nucleotides
pfkA and sbp are separated by 319 nucleotides
b3912: cpxR - DNA-binding response regulator in two-component regulatory system with CpxA (NCBI), at 4,102,995 to 4,103,693
cpxR
b4484: cpxP - periplasmic protein combats stress (NCBI), at 4,103,843 to 4,104,343
cpxP
b3915: fieF - ferrous iron efflux protein F (NCBI), at 4,104,492 to 4,105,394
fieF
b3916: pfkA - 6-phosphofructokinase (NCBI), at 4,105,575 to 4,106,537
pfkA
b3917: sbp - sulfate transporter subunit (NCBI), at 4,106,857 to 4,107,846
sbp
Group
Condition
b3912
b4484
b3915
b3916
b3917
cpxR
cpxP
fieF
pfkA
sbp
stress
octanol 0.08 vol%
+0.0
-2.3
+0.2
-4.1
+0.1
nutrient
M9 Glucose 0.2%, Thauer's vit mix
-0.1
-0.3
-0.5
-4.5
+0.0
carbon source
Ying_sugar7 rep C; time point 3
-0.1
-0.2
-0.1
-3.5
-1.4
carbon source
Ying_sugar7 rep B; time point 3
-0.0
-0.2
+0.0
-3.7
-1.4
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-5.5
+1.1
+0.1
-0.9
+0.1
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
-4.0
+1.0
-0.4
-1.4
-0.2
carbon source
Ying_sugar7 rep A; time point 3
-0.2
-0.2
-0.1
-3.6
-0.8
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-5.5
+1.1
-0.4
-0.3
+0.2
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
-0.8
-0.6
+0.3
-3.2
-0.4
motility
outer cut, LB soft agar motility assay
-0.1
+0.4
-0.9
-3.9
-0.1
control
PBS_cage_2_mouse_2_Cecum_40 hr
-1.2
-0.3
+0.1
-3.4
+0.3
stress
2,4-Dinitrophenol 2 mM
+1.3
-1.6
-0.0
-3.8
+0.1
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
-3.1
+1.1
-0.8
-1.3
+0.1
control
PBS_cage_2_mice _Feces_40 hr
-0.8
+0.1
+0.1
-3.7
+0.3
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-4.9
+2.2
-0.1
-1.0
+0.0
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-3.8
+1.3
-0.4
-0.9
+0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-5.6
+2.5
-0.0
-0.4
-0.1
stress
2,4-Dinitrophenol 2 mM
+1.4
-1.3
+0.0
-3.3
+0.1
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-4.2
+1.6
-0.5
-0.0
+0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-5.1
+1.9
-0.1
+0.1
+0.3
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-5.0
+1.0
+0.3
+0.6
+0.3
carbon source
a-Ketoglutaric (C)
-2.2
+1.3
-0.6
-1.0
+0.1
carbon source
a-Ketoglutaric (C)
-1.8
+1.4
-0.5
-1.3
+0.2
stress
LB Aerobic with Ramoplanin 0.000125 mM
-2.8
+1.6
+0.5
+0.2
+0.0
stress
Aluminum chloride 5 mM
-2.3
+0.0
+0.2
+2.6
+0.1
stress
Benzalkonium Chloride 0.004 mg/ml
+4.0
-2.2
+0.5
-1.5
-0.0
stress
Nickel (II) chloride 1 mM
-0.4
+1.0
+2.2
-1.3
+0.1
stress
Cephalothin 0.01 mg/ml
-1.8
+1.1
+0.4
+1.9
+0.2
stress
Nickel (II) chloride 1.5 mM
-0.7
+1.0
+2.7
-1.1
+0.0
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
+0.7
-0.3
-0.6
+4.2
+0.2
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