Fitness for 5 genes in Escherichia coli BW25113

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500 ntcpxR and cpxP are separated by 149 nucleotidescpxP and fieF are separated by 148 nucleotidesfieF and pfkA are separated by 180 nucleotidespfkA and sbp are separated by 319 nucleotides b3912: cpxR - DNA-binding response regulator in two-component regulatory system with CpxA (NCBI), at 4,102,995 to 4,103,693 cpxR b4484: cpxP - periplasmic protein combats stress (NCBI), at 4,103,843 to 4,104,343 cpxP b3915: fieF - ferrous iron efflux protein F (NCBI), at 4,104,492 to 4,105,394 fieF b3916: pfkA - 6-phosphofructokinase (NCBI), at 4,105,575 to 4,106,537 pfkA b3917: sbp - sulfate transporter subunit (NCBI), at 4,106,857 to 4,107,846 sbp
Group Condition b3912 b4484 b3915 b3916 b3917
cpxR cpxP fieF pfkA sbp
stress octanol 0.08 vol% +0.0 -2.3 +0.2 -4.1 +0.1
nutrient M9 Glucose 0.2%, Thauer's vit mix -0.1 -0.3 -0.5 -4.5 +0.0
carbon source Ying_sugar7 rep C; time point 3 -0.1 -0.2 -0.1 -3.5 -1.4
carbon source Ying_sugar7 rep B; time point 3 -0.0 -0.2 +0.0 -3.7 -1.4
plasmid transformation mariner vector transformation; liquid outgrowth assay -5.5 +1.1 +0.1 -0.9 +0.1
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM -4.0 +1.0 -0.4 -1.4 -0.2
carbon source Ying_sugar7 rep A; time point 3 -0.2 -0.2 -0.1 -3.6 -0.8
plasmid transformation mariner vector transformation; liquid outgrowth assay -5.5 +1.1 -0.4 -0.3 +0.2
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr -0.8 -0.6 +0.3 -3.2 -0.4
motility outer cut, LB soft agar motility assay -0.1 +0.4 -0.9 -3.9 -0.1
control PBS_cage_2_mouse_2_Cecum_40 hr -1.2 -0.3 +0.1 -3.4 +0.3
stress 2,4-Dinitrophenol 2 mM +1.3 -1.6 -0.0 -3.8 +0.1
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM -3.1 +1.1 -0.8 -1.3 +0.1
control PBS_cage_2_mice _Feces_40 hr -0.8 +0.1 +0.1 -3.7 +0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay -4.9 +2.2 -0.1 -1.0 +0.0
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -3.8 +1.3 -0.4 -0.9 +0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay -5.6 +2.5 -0.0 -0.4 -0.1
stress 2,4-Dinitrophenol 2 mM +1.4 -1.3 +0.0 -3.3 +0.1
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -4.2 +1.6 -0.5 -0.0 +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay -5.1 +1.9 -0.1 +0.1 +0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay -5.0 +1.0 +0.3 +0.6 +0.3
carbon source a-Ketoglutaric (C) -2.2 +1.3 -0.6 -1.0 +0.1
carbon source a-Ketoglutaric (C) -1.8 +1.4 -0.5 -1.3 +0.2
stress LB Aerobic with Ramoplanin 0.000125 mM -2.8 +1.6 +0.5 +0.2 +0.0
stress Aluminum chloride 5 mM -2.3 +0.0 +0.2 +2.6 +0.1
stress Benzalkonium Chloride 0.004 mg/ml +4.0 -2.2 +0.5 -1.5 -0.0
stress Nickel (II) chloride 1 mM -0.4 +1.0 +2.2 -1.3 +0.1
stress Cephalothin 0.01 mg/ml -1.8 +1.1 +0.4 +1.9 +0.2
stress Nickel (II) chloride 1.5 mM -0.7 +1.0 +2.7 -1.1 +0.0
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +0.7 -0.3 -0.6 +4.2 +0.2
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