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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
fdoH and fdoG are separated by 12 nucleotides
fdoG and fdhD are separated by 193 nucleotides
fdhD and yiiG are separated by 152 nucleotides
yiiG and frvR are separated by 49 nucleotides
b3893: fdoH - formate dehydrogenase-O, Fe-S subunit (NCBI), at 4,079,880 to 4,080,782
fdoH
b3894: fdoG - formate dehydrogenase-O, large subunit (NCBI), at 4,080,795 to 4,083,845
fdoG
b3895: fdhD - formate dehydrogenase accessory protein (NCBI), at 4,084,039 to 4,084,872
fdhD
b3896: yiiG - hypothetical protein (NCBI), at 4,085,025 to 4,086,080
yiiG
b3897: frvR - predicted regulator (NCBI), at 4,086,130 to 4,087,878
frvR
Group
Condition
b3893
b3894
b3895
b3896
b3897
fdoH
fdoG
fdhD
yiiG
frvR
stress
2,4-Dinitrophenol 2 mM
-2.2
-2.4
-2.3
+0.1
+0.1
stress
2,4-Dinitrophenol 2 mM
-1.7
-1.8
-2.3
+0.1
+0.0
stress
octanol 0.08 vol%
-1.5
-1.1
-1.6
-0.2
+0.2
stress
LB Aerobic with Irgasan 0.0000003125 mM
-0.8
-0.9
-0.1
+0.3
+0.1
phage
Phage_N4_cage_4_mouse_1_Cecum_40 hr
-0.4
-0.2
-1.1
+0.4
+0.0
phage
Ste41
+0.4
+0.3
-0.5
-0.6
-0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.4
+0.8
+0.1
-0.5
-0.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.7
+0.9
+1.1
-0.1
-0.3
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.0
+0.8
+1.0
-0.4
-0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.3
+0.7
+1.0
-0.2
-0.3
plasmid transformation
vcDART vector transformation; agar assay
+0.3
+0.6
+0.8
-0.2
-0.1
plasmid transformation
vcDART vector transformation; agar assay
+0.3
+0.6
+0.8
-0.1
-0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.3
+0.9
+0.8
-0.3
-0.2
motility
outer cut, LB soft agar motility assay
+0.3
+0.7
+1.1
-0.3
-0.1
stress
LB Aerobic with Hygromycin B 0.000015625 mM
+0.6
+0.5
+0.8
-0.1
+0.0
stress
toluene 2.5 vol%
+0.6
+0.9
+0.7
-0.0
-0.0
motility
outer cut, LB soft agar motility assay
+0.8
+0.8
+1.0
-0.2
-0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.1
+1.4
+1.1
+0.3
-0.2
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+1.0
+1.2
+0.8
-0.4
+0.1
stress
2-Furfuraldehyde 0.25 vol%
+0.8
+1.0
+1.1
-0.1
+0.0
stress
1-ethyl-3-methylimidazolium chloride 80 mM
+1.0
+1.2
+1.0
-0.1
-0.1
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+1.3
+1.1
+0.7
-0.2
+0.1
stress
LB Aerobic with Nitrofurantoin 0.00003125 mM
+1.0
+1.1
+1.3
-0.1
-0.1
stress
Cisplatin 0.1 mg/ml
+1.1
+1.5
+1.0
-0.1
-0.0
stress
1-ethyl-3-methylimidazolium chloride 40 mM
+1.3
+1.3
+1.3
-0.2
-0.0
stress
LB Anaerobic with Phenazine ethosulfate 0.000125 mM
+1.6
+1.5
+1.4
-0.2
+0.1
stress
Spectinomycin 0.00625 mg/ml
+1.4
+1.6
+1.6
-0.0
+0.0
stress
Benzalkonium Chloride 0.004 mg/ml
+1.7
+1.9
+1.7
-0.3
-0.0
stress
LB Anaerobic with Phenazine ethosulfate 0.000125 mM
+2.2
+2.2
+2.0
-0.2
+0.0
stress
Spectinomycin 0.0125 mg/ml
+1.9
+2.3
+2.2
-0.0
-0.0
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