Fitness for 5 genes in Escherichia coli BW25113

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500 ntrmuC and ubiE are separated by 94 nucleotidesubiE and yigP are separated by 13 nucleotidesyigP and ubiB overlap by 4 nucleotidesubiB and b3836 are separated by 78 nucleotides b3832: rmuC - predicted recombination limiting protein (NCBI), at 4,015,356 to 4,016,783 rmuC b3833: ubiE - ubiquinone/menaquinone biosynthesis methyltransferase (NCBI), at 4,016,878 to 4,017,633 ubiE b3834: yigP - hypothetical protein (NCBI), at 4,017,647 to 4,018,252 yigP b3835: ubiB - putative ubiquinone biosynthesis protein UbiB (NCBI), at 4,018,249 to 4,019,889 ubiB b3836: b3836 - orf, hypothetical protein (VIMSS), at 4,019,968 to 4,020,237 b3836
Group Condition b3832 b3833 b3834 b3835 b3836
rmuC ubiE yigP ubiB b3836
phage Δdbn_10 -1.2 N.D. -0.2 N.D. -3.0
stress 2,4-Dinitrophenol 2 mM +0.0 N.D. -1.1 N.D. -2.6
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.1 N.D. -0.1 N.D. -3.0
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.1 N.D. -0.6 N.D. -2.3
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.1 N.D. -0.2 N.D. -2.5
stress 2,4-Dinitrophenol 2 mM -0.1 N.D. -1.0 N.D. -1.7
control PBS_cage_2_mouse_3_Cecum_40 hr -0.0 N.D. -0.4 N.D. -2.1
control PBS_cage_1_mice _Feces_40 hr +0.2 N.D. +0.3 N.D. -2.9
phage TP4 +0.1 N.D. -0.8 N.D. -1.7
phage Phage_N4_cage_4_mouse_1_Cecum_40 hr -0.0 N.D. -0.2 N.D. -2.3
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.1 N.D. -1.3 N.D. -1.1
control PBS_cage_2_mice _Feces_40 hr +0.1 N.D. -0.3 N.D. -2.1
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM -0.1 N.D. +0.5 N.D. -2.6
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.0 N.D. +0.4 N.D. -2.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.2 N.D. +1.0 N.D. -2.6
phage RB68 +0.4 N.D. -0.0 N.D. -1.9
control PBS_cage_1_mice _Feces_22 hr +0.1 N.D. +0.4 N.D. -1.9
stress LB Anaerobic with Chloramphenicol 0.000001953125 mM -0.1 N.D. +0.6 N.D. -1.9
control PBS_cage_1_mouse_2_Cecum_40 hr +0.2 N.D. +0.4 N.D. -1.9
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.3 N.D. +1.6 N.D. -1.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.2 N.D. +1.1 N.D. -1.0
stress Chlorite 1.25 mM -0.2 N.D. -0.9 N.D. +1.7
stress pinene 0.3125 vol% +0.8 N.D. +1.4 N.D. -1.0
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.8 N.D. +0.1 N.D. +2.1
carbon source carbon source D-Mannose 1mM +0.4 N.D. -0.3 N.D. +1.9
phage T7_phage 0.00015625 MOI TC4 +0.6 N.D. -0.3 N.D. +2.0
stress Spectinomycin 0.0125 mg/ml -0.2 N.D. +1.0 N.D. +1.6
stress limonene 5 vol% +0.4 N.D. +1.0 N.D. +1.7
phage T7_phage 0.00015625 MOI TC3 +1.0 N.D. +0.1 N.D. +4.1
phage T7_phage 0.0015625 MOI TC2 +1.1 N.D. -0.1 N.D. +4.6
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