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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
rarD and yigI are separated by 51 nucleotides
yigI and pldA are separated by 164 nucleotides
pldA and recQ are separated by 132 nucleotides
recQ and rhtC are separated by 63 nucleotides
b3819: rarD - predicted chloramphenical resistance permease (NCBI), at 4,001,311 to 4,002,201
rarD
b3820: yigI - orf, hypothetical protein (VIMSS), at 4,002,253 to 4,002,720
yigI
b3821: pldA - outer membrane phospholipase A (NCBI), at 4,002,885 to 4,003,754
pldA
b3822: recQ - ATP-dependent DNA helicase (NCBI), at 4,003,887 to 4,005,716
recQ
b3823: rhtC - threonine efflux system (NCBI), at 4,005,780 to 4,006,400
rhtC
Group
Condition
b3819
b3820
b3821
b3822
b3823
rarD
yigI
pldA
recQ
rhtC
stress
LB Aerobic with Irgasan 0.0000003125 mM
+0.3
+0.9
-3.7
-0.3
-0.2
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.2
+0.1
-1.8
-0.5
-0.6
stress
LB Aerobic with Nitrofurantoin 0.00003125 mM
-0.5
+0.3
-0.0
-1.9
+0.1
stress
LB Anaerobic with Benzethonium chloride 0.000015625 mM
+0.1
-0.2
-1.7
-0.2
+0.0
stress
LB Aerobic with Benzethonium chloride 0.000015625 mM
+0.0
-0.1
-1.6
-0.1
-0.0
stress
LB Anaerobic with Irgasan 0.000000625 mM
+0.1
+0.0
-1.6
-0.1
-0.0
carbon source
carbon source D-Mannose 5mM
-0.0
-0.4
-0.7
-0.2
-0.2
stress
Cisplatin 0.1 mg/ml
-0.0
+0.0
+0.0
-1.3
-0.2
motility
outer cut, LB soft agar motility assay
-0.0
-0.5
+0.1
-0.2
-0.8
stress
LB Anaerobic with Irgasan 0.000000625 mM
+0.1
+0.0
-1.5
-0.1
+0.1
phage
R2_N4_T1_phage10
-0.2
-0.5
-0.3
-0.2
-0.2
ecofab
LB 0.5x in EcoFAB_3.5mL
-0.0
+0.2
-1.2
+0.0
-0.2
phage
Bas60
-0.0
+0.2
-0.8
-0.1
-0.3
ecofab
LB in EcoFAB_3.5mL
-0.0
+0.1
-1.0
-0.1
-0.1
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+0.0
+0.1
-0.8
-0.2
-0.2
stress
LB Anaerobic with Benzethonium chloride 0.000015625 mM
+0.1
+0.1
-1.2
-0.0
-0.0
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.5
+0.2
+0.1
-0.0
-0.6
stress
LB Anaerobic with Furaltadone 0.000015625 mM
+0.3
-0.4
-0.6
-0.1
+0.2
stress
LB Anaerobic with Nitrofurantoin 0.000015625 mM
-0.2
+0.6
-0.1
-0.5
-0.3
stress
methylglyoxal 0.008 vol%
+0.0
-0.4
-0.4
+0.5
-0.1
stress
Cisplatin 0.1 mg/ml
-0.0
-0.3
-0.6
+0.9
-0.3
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.2
+0.1
+0.2
+0.4
-0.5
stress
3-methyl-1-butanol 1.25 vol%
+0.2
-0.3
+0.4
-0.2
+0.3
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
-0.9
+1.7
-0.3
-0.3
+0.3
stress
Ciprofloxacin Hydrochloride 0.0003 mM
+0.0
-0.3
+0.8
+0.1
-0.1
phage
T7_phage 0.00015625 MOI TC3
+0.1
-0.1
+0.1
+1.0
-0.3
stress
Cephalothin 0.01 mg/ml
+0.2
+0.5
-0.3
+0.4
-0.1
phage
T7_phage 0.0015625 MOI TC2
-0.0
-0.2
+0.2
+0.6
+0.4
micoeukaryotes
Tetrahymena sp., Mixed culture-1
+0.2
+0.6
+0.3
-0.1
+0.2
phage
Papa dilution 10-1 cacl2 maltose
+0.0
+0.0
+0.5
+0.7
+0.2
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