Fitness for 5 genes in Escherichia coli BW25113

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500 ntrarD and yigI are separated by 51 nucleotidesyigI and pldA are separated by 164 nucleotidespldA and recQ are separated by 132 nucleotidesrecQ and rhtC are separated by 63 nucleotides b3819: rarD - predicted chloramphenical resistance permease (NCBI), at 4,001,311 to 4,002,201 rarD b3820: yigI - orf, hypothetical protein (VIMSS), at 4,002,253 to 4,002,720 yigI b3821: pldA - outer membrane phospholipase A (NCBI), at 4,002,885 to 4,003,754 pldA b3822: recQ - ATP-dependent DNA helicase (NCBI), at 4,003,887 to 4,005,716 recQ b3823: rhtC - threonine efflux system (NCBI), at 4,005,780 to 4,006,400 rhtC
Group Condition b3819 b3820 b3821 b3822 b3823
rarD yigI pldA recQ rhtC
stress LB Aerobic with Irgasan 0.0000003125 mM +0.3 +0.9 -3.7 -0.3 -0.2
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.2 +0.1 -1.8 -0.5 -0.6
stress LB Aerobic with Nitrofurantoin 0.00003125 mM -0.5 +0.3 -0.0 -1.9 +0.1
stress LB Anaerobic with Benzethonium chloride 0.000015625 mM +0.1 -0.2 -1.7 -0.2 +0.0
stress LB Aerobic with Benzethonium chloride 0.000015625 mM +0.0 -0.1 -1.6 -0.1 -0.0
stress LB Anaerobic with Irgasan 0.000000625 mM +0.1 +0.0 -1.6 -0.1 -0.0
carbon source carbon source D-Mannose 5mM -0.0 -0.4 -0.7 -0.2 -0.2
stress Cisplatin 0.1 mg/ml -0.0 +0.0 +0.0 -1.3 -0.2
motility outer cut, LB soft agar motility assay -0.0 -0.5 +0.1 -0.2 -0.8
stress LB Anaerobic with Irgasan 0.000000625 mM +0.1 +0.0 -1.5 -0.1 +0.1
phage R2_N4_T1_phage10 -0.2 -0.5 -0.3 -0.2 -0.2
ecofab LB 0.5x in EcoFAB_3.5mL -0.0 +0.2 -1.2 +0.0 -0.2
phage Bas60 -0.0 +0.2 -0.8 -0.1 -0.3
ecofab LB in EcoFAB_3.5mL -0.0 +0.1 -1.0 -0.1 -0.1
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +0.0 +0.1 -0.8 -0.2 -0.2
stress LB Anaerobic with Benzethonium chloride 0.000015625 mM +0.1 +0.1 -1.2 -0.0 -0.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.5 +0.2 +0.1 -0.0 -0.6
stress LB Anaerobic with Furaltadone 0.000015625 mM +0.3 -0.4 -0.6 -0.1 +0.2
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM -0.2 +0.6 -0.1 -0.5 -0.3
stress methylglyoxal 0.008 vol% +0.0 -0.4 -0.4 +0.5 -0.1
stress Cisplatin 0.1 mg/ml -0.0 -0.3 -0.6 +0.9 -0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.2 +0.1 +0.2 +0.4 -0.5
stress 3-methyl-1-butanol 1.25 vol% +0.2 -0.3 +0.4 -0.2 +0.3
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -0.9 +1.7 -0.3 -0.3 +0.3
stress Ciprofloxacin Hydrochloride 0.0003 mM +0.0 -0.3 +0.8 +0.1 -0.1
phage T7_phage 0.00015625 MOI TC3 +0.1 -0.1 +0.1 +1.0 -0.3
stress Cephalothin 0.01 mg/ml +0.2 +0.5 -0.3 +0.4 -0.1
phage T7_phage 0.0015625 MOI TC2 -0.0 -0.2 +0.2 +0.6 +0.4
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.2 +0.6 +0.3 -0.1 +0.2
phage Papa dilution 10-1 cacl2 maltose +0.0 +0.0 +0.5 +0.7 +0.2
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