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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
hemC and cyaA are separated by 386 nucleotides
cyaA and cyaY are separated by 39 nucleotides
cyaY and yifL are separated by 462 nucleotides
yifL and dapF are separated by 36 nucleotides
b3805: hemC - porphobilinogen deaminase (NCBI), at 3,987,848 to 3,988,789
hemC
b3806: cyaA - adenylate cyclase (NCBI), at 3,989,176 to 3,991,722
cyaA
b3807: cyaY - frataxin-like protein (NCBI), at 3,991,762 to 3,992,082
cyaY
b4558: yifL - predicted lipoprotein (NCBI), at 3,992,545 to 3,992,748
yifL
b3809: dapF - diaminopimelate epimerase (VIMSS), at 3,992,785 to 3,993,609
dapF
Group
Condition
b3805
b3806
b3807
b4558
b3809
hemC
cyaA
cyaY
yifL
dapF
carbon source
Glycolic Acid (C)
N.D.
-3.0
-2.8
-0.7
N.D.
carbon source
Glycolic Acid (C)
N.D.
-3.2
-2.4
-0.2
N.D.
carbon source
L-Malic (C)
N.D.
-3.3
-1.5
-0.8
N.D.
carbon source
succinate (C)
N.D.
-3.4
-1.3
-0.6
N.D.
carbon source
succinate (C)
N.D.
-3.2
-1.5
-0.5
N.D.
carbon source
L-Malic (C)
N.D.
-3.4
-1.5
-0.2
N.D.
carbon source
D-Maltose (C)
N.D.
-3.2
-0.8
-0.5
N.D.
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
N.D.
+2.0
+0.2
-3.5
N.D.
stress
Cephalothin 0.01 mg/ml
N.D.
+2.8
-1.8
+0.5
N.D.
stress
Carbenicillin 0.008 mg/ml
N.D.
+3.5
-1.6
+0.5
N.D.
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
N.D.
+1.4
-1.1
+2.1
N.D.
stress
Aluminum chloride 5 mM
N.D.
+3.9
-0.2
-0.7
N.D.
phage
Phage_N4_cage_3_mouse_3_Cecum_40 hr
N.D.
+3.1
+0.6
-0.8
N.D.
stress
Chlorite 1.25 mM
N.D.
+3.8
-0.4
+0.3
N.D.
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
N.D.
+3.9
+0.2
-0.2
N.D.
plasmid transformation
vcDART vector transformation; agar assay
N.D.
+2.6
+1.8
-0.5
N.D.
stress
LB Anaerobic with Nitrofurantoin 0.000015625 mM
N.D.
+3.9
+0.6
-0.2
N.D.
plasmid transformation
mariner vector transformation; liquid outgrowth assay
N.D.
+3.0
+1.4
+0.1
N.D.
stress
Cisplatin 0.1 mg/ml
N.D.
+4.1
+0.7
-0.3
N.D.
stress
LB Anaerobic with Furaltadone 0.000015625 mM
N.D.
+4.7
-0.5
+0.5
N.D.
carbon source
D-Serine (C)
N.D.
+5.2
-0.1
-0.4
N.D.
stress
LB Anaerobic with Furaltadone 0.000015625 mM
N.D.
+4.4
-0.2
+0.5
N.D.
carbon source
D-Serine (C)
N.D.
+5.0
-0.2
+0.0
N.D.
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
N.D.
+5.0
+0.8
-0.4
N.D.
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
N.D.
+5.6
+0.9
-1.0
N.D.
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
N.D.
+5.5
+0.6
-0.0
N.D.
plasmid transformation
mariner vector transformation; liquid outgrowth assay
N.D.
+4.2
+2.5
-0.6
N.D.
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
N.D.
+5.6
+0.7
+0.1
N.D.
plasmid transformation
mariner vector transformation; liquid outgrowth assay
N.D.
+5.2
+2.1
+0.0
N.D.
stress
Phosphomycin 0.001 mg/ml
N.D.
+7.7
-0.2
+0.2
N.D.
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