Fitness for 5 genes in Escherichia coli BW25113

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500 nthemC and cyaA are separated by 386 nucleotidescyaA and cyaY are separated by 39 nucleotidescyaY and yifL are separated by 462 nucleotidesyifL and dapF are separated by 36 nucleotides b3805: hemC - porphobilinogen deaminase (NCBI), at 3,987,848 to 3,988,789 hemC b3806: cyaA - adenylate cyclase (NCBI), at 3,989,176 to 3,991,722 cyaA b3807: cyaY - frataxin-like protein (NCBI), at 3,991,762 to 3,992,082 cyaY b4558: yifL - predicted lipoprotein (NCBI), at 3,992,545 to 3,992,748 yifL b3809: dapF - diaminopimelate epimerase (VIMSS), at 3,992,785 to 3,993,609 dapF
Group Condition b3805 b3806 b3807 b4558 b3809
hemC cyaA cyaY yifL dapF
carbon source Glycolic Acid (C) N.D. -3.0 -2.8 -0.7 N.D.
carbon source Glycolic Acid (C) N.D. -3.2 -2.4 -0.2 N.D.
carbon source L-Malic (C) N.D. -3.3 -1.5 -0.8 N.D.
carbon source succinate (C) N.D. -3.4 -1.3 -0.6 N.D.
carbon source succinate (C) N.D. -3.2 -1.5 -0.5 N.D.
carbon source L-Malic (C) N.D. -3.4 -1.5 -0.2 N.D.
carbon source D-Maltose (C) N.D. -3.2 -0.8 -0.5 N.D.
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM N.D. +2.0 +0.2 -3.5 N.D.
stress Cephalothin 0.01 mg/ml N.D. +2.8 -1.8 +0.5 N.D.
stress Carbenicillin 0.008 mg/ml N.D. +3.5 -1.6 +0.5 N.D.
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM N.D. +1.4 -1.1 +2.1 N.D.
stress Aluminum chloride 5 mM N.D. +3.9 -0.2 -0.7 N.D.
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr N.D. +3.1 +0.6 -0.8 N.D.
stress Chlorite 1.25 mM N.D. +3.8 -0.4 +0.3 N.D.
stress LB Anaerobic with Amoxicillin 0.0000078125 mM N.D. +3.9 +0.2 -0.2 N.D.
plasmid transformation vcDART vector transformation; agar assay N.D. +2.6 +1.8 -0.5 N.D.
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM N.D. +3.9 +0.6 -0.2 N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. +3.0 +1.4 +0.1 N.D.
stress Cisplatin 0.1 mg/ml N.D. +4.1 +0.7 -0.3 N.D.
stress LB Anaerobic with Furaltadone 0.000015625 mM N.D. +4.7 -0.5 +0.5 N.D.
carbon source D-Serine (C) N.D. +5.2 -0.1 -0.4 N.D.
stress LB Anaerobic with Furaltadone 0.000015625 mM N.D. +4.4 -0.2 +0.5 N.D.
carbon source D-Serine (C) N.D. +5.0 -0.2 +0.0 N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. +5.0 +0.8 -0.4 N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. +5.6 +0.9 -1.0 N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. +5.5 +0.6 -0.0 N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. +4.2 +2.5 -0.6 N.D.
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM N.D. +5.6 +0.7 +0.1 N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. +5.2 +2.1 +0.0 N.D.
stress Phosphomycin 0.001 mg/ml N.D. +7.7 -0.2 +0.2 N.D.
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