Fitness for 5 genes in Escherichia coli BW25113

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500 ntaslA and ryiA are separated by 424 nucleotidesryiA and hemY are separated by 82 nucleotideshemY and hemX are separated by 2 nucleotideshemX and hemD are separated by 21 nucleotides b3801: aslA - acrylsulfatase-like enzyme (NCBI), at 3,982,375 to 3,984,030 aslA b4456: ryiA - regulatory sRNA (NCBI), at 3,984,455 to 3,984,626 ryiA b3802: hemY - predicted protoheme IX synthesis protein (NCBI), at 3,984,709 to 3,985,905 hemY b3803: hemX - predicted uroporphyrinogen III methylase (NCBI), at 3,985,908 to 3,987,089 hemX b3804: hemD - uroporphyrinogen-III synthetase (NCBI), at 3,987,111 to 3,987,851 hemD
Group Condition b3801 b4456 b3802 b3803 b3804
aslA ryiA hemY hemX hemD
stress 2,4-Dinitrophenol 2 mM +0.0 -0.1 -2.1 -3.3 N.D.
stress 2,4-Dinitrophenol 2 mM -0.0 -0.2 -2.4 -2.8 N.D.
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM +0.0 +0.2 -0.7 -2.8 N.D.
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM -0.1 +0.2 -0.7 -2.5 N.D.
stress 1-ethyl-3-methylimidazolium chloride 80 mM +0.0 -0.3 -0.8 -2.1 N.D.
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.0 -0.1 -0.2 -2.3 N.D.
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.1 +0.3 -0.9 -2.0 N.D.
sir2-hera SIR2-HerA +0.1 +0.1 -0.8 -1.7 N.D.
retron_ec_48_control retron_Ec_48_control +0.2 +0.2 -0.9 -1.7 N.D.
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +0.1 +0.1 -0.2 -2.3 N.D.
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.1 -0.4 +0.1 -1.9 N.D.
sir2-hera SIR2-HerA +0.2 +0.2 -0.8 -1.5 N.D.
retron_ec_48_control retron_Ec_48_control +0.1 +0.3 -0.9 -1.5 N.D.
sir2-hera SIR2-HerA +0.2 +0.2 -0.8 -1.5 N.D.
mzaabcde mzaABCDE +0.3 -0.3 -0.7 -1.1 N.D.
ppi ppI +0.1 +0.3 -0.7 -1.4 N.D.
drt_type_3 DRT_type_3 +0.2 +0.3 -0.8 -1.3 N.D.
motility outer cut, LB soft agar motility assay +0.1 +0.5 -0.8 -1.3 N.D.
mzaabcde mzaABCDE +0.2 +0.4 -0.6 -1.3 N.D.
stress Benzalkonium Chloride 0.004 mg/ml -0.1 -0.6 +1.1 -1.5 N.D.
phage Δdbn_10 +0.1 +0.5 -0.5 -1.2 N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.1 -0.5 +1.0 +1.1 N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.1 -0.5 +0.9 +1.5 N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.2 -0.2 +1.1 +1.4 N.D.
stress Bacitracin 1 mg/ml -0.1 +0.1 +0.8 +1.5 N.D.
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.1 -0.6 +1.4 +1.7 N.D.
stress Aluminum chloride 5 mM +0.1 -0.0 +0.8 +2.0 N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.0 -0.3 +1.4 +1.9 N.D.
stress toluene 2.5 vol% +0.2 -0.0 +1.2 +1.8 N.D.
stress toluene 2.5 vol% -0.1 +0.4 +1.2 +1.6 N.D.
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