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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
rho and rfe are separated by 239 nucleotides
rfe and wzzE are separated by 11 nucleotides
wzzE and rffE are separated by 55 nucleotides
rffE and rffD overlap by 4 nucleotides
b3783: rho - transcription termination factor Rho (NCBI), at 3,964,440 to 3,965,699
rho
b3784: rfe - UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI), at 3,965,939 to 3,967,042
rfe
b3785: wzzE - putative transport protein (VIMSS), at 3,967,054 to 3,968,100
wzzE
b3786: rffE - UDP-N-acetyl glucosamine-2-epimerase (NCBI), at 3,968,156 to 3,969,286
rffE
b3787: rffD - UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI), at 3,969,283 to 3,970,545
rffD
Group
Condition
b3783
b3784
b3785
b3786
b3787
rho
rfe
wzzE
rffE
rffD
micoeukaryotes
Tetrahymena sp., Mixed culture-1
N.D.
-6.6
-2.3
-4.1
-5.5
stress
octanol 0.08 vol%
N.D.
-5.8
-1.7
-5.1
-5.5
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
N.D.
-4.1
-3.4
-4.4
-4.3
mzaabcde
mzaABCDE
N.D.
-5.5
-1.0
-4.4
-4.8
mzaabcde
mzaABCDE
N.D.
-4.4
-0.7
-5.7
-4.8
mzaabcde
mzaABCDE
N.D.
-4.2
-1.0
-4.4
-5.5
ppi
ppI
N.D.
-4.6
-1.0
-4.6
-4.8
stress
2,4-Dinitrophenol 2 mM
N.D.
-4.8
-1.5
-4.2
-4.3
drt_type_3
DRT_type_3
N.D.
-4.9
-0.7
-4.3
-4.7
sir2-hera
SIR2-HerA
N.D.
-4.7
-0.8
-4.2
-4.3
retron_ec_48_control
retron_Ec_48_control
N.D.
-4.5
-0.8
-4.5
-4.2
drt_type_3
DRT_type_3
N.D.
-4.0
-0.8
-4.6
-4.2
sir2-hera
SIR2-HerA
N.D.
-4.2
-1.0
-4.2
-4.1
gfp_control
GFP_control
N.D.
-4.0
-0.7
-4.0
-4.7
gfp_control
GFP_control
N.D.
-4.2
-0.6
-4.2
-4.1
gfp_control
GFP_control
N.D.
-3.8
-0.9
-4.3
-4.2
ppi
ppI
N.D.
-4.1
-1.0
-4.1
-3.8
drt_type_3
DRT_type_3
N.D.
-3.9
-0.9
-4.0
-4.1
ppi
ppI
N.D.
-3.9
-1.0
-3.9
-4.1
retron_ec_48_control
retron_Ec_48_control
N.D.
-4.5
-0.6
-4.1
-3.6
stress
2,4-Dinitrophenol 2 mM
N.D.
-3.7
-1.5
-3.8
-3.7
sir2-hera
SIR2-HerA
N.D.
-3.7
-0.9
-4.5
-3.6
retron_ec_48_control
retron_Ec_48_control
N.D.
-3.9
-1.0
-3.9
-3.3
stress
Benzalkonium Chloride 0.004 mg/ml
N.D.
-3.6
-1.7
-3.3
-3.4
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
N.D.
-3.6
-0.7
-3.9
-3.6
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
N.D.
-3.7
-0.6
-3.8
-3.7
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
N.D.
-3.4
-0.7
-3.4
-3.7
stress
LB Anaerobic with Benzethonium chloride 0.000015625 mM
N.D.
-3.7
-1.2
-2.9
-3.2
motility
outer cut, LB soft agar motility assay
N.D.
-3.4
-0.3
-3.5
-3.6
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
N.D.
+2.9
+2.1
+3.1
+3.0
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