Fitness for 5 genes in Escherichia coli BW25113

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500 ntrep and gpp are separated by 46 nucleotidesgpp and rhlB are separated by 135 nucleotidesrhlB and trxA are separated by 130 nucleotidestrxA and rho are separated by 326 nucleotides b3778: rep - DNA helicase and single-stranded DNA-dependent ATPase (NCBI), at 3,958,700 to 3,960,721 rep b3779: gpp - guanosine pentaphosphatase/exopolyphosphatase (NCBI), at 3,960,768 to 3,962,252 gpp b3780: rhlB - ATP-dependent RNA helicase (NCBI), at 3,962,388 to 3,963,653 rhlB b3781: trxA - thioredoxin 1 (VIMSS), at 3,963,784 to 3,964,113 trxA b3783: rho - transcription termination factor Rho (NCBI), at 3,964,440 to 3,965,699 rho
Group Condition b3778 b3779 b3780 b3781 b3783
rep gpp rhlB trxA rho
carbon source carbon source D-Mannose 1mM -1.0 -0.8 +0.1 -6.3 N.D.
phage Lambda_ci1857 -2.5 -0.7 -1.5 -3.0 N.D.
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) -1.1 -1.3 -0.5 -4.9 N.D.
carbon source carbon source D-Mannose 0.5mM -1.2 -0.5 +0.2 -6.0 N.D.
carbon source carbon source D-Mannose 1mM -0.6 -1.0 +0.3 -6.2 N.D.
carbon source Ying_Nucleobase14 rep B; time point 3 +0.3 -0.8 -0.1 -6.9 N.D.
mzaabcde mzaABCDE -4.2 +0.0 -0.7 -2.5 N.D.
ppi ppI -4.2 +0.3 -0.8 -2.5 N.D.
carbon source Ying_sugar7 rep C; time point 3 +1.8 -1.5 -0.6 -6.9 N.D.
retron_ec_48_control retron_Ec_48_control -4.1 +0.6 -0.6 -3.1 N.D.
carbon source Ying_sugar7 rep A; time point 3 +1.9 -1.3 -0.6 -7.0 N.D.
mzaabcde mzaABCDE -3.9 +0.2 -0.6 -2.4 N.D.
carbon source Ying_sugar7 rep B; time point 3 +1.8 -1.3 -0.6 -6.6 N.D.
motility outer cut, LB soft agar motility assay -4.7 +0.8 -1.6 -1.0 N.D.
ppi ppI -3.7 +0.4 -0.7 -2.4 N.D.
gfp_control GFP_control -3.8 +0.4 -0.8 -2.2 N.D.
gfp_control GFP_control -3.5 +0.6 -0.7 -2.5 N.D.
motility outer cut, LB soft agar motility assay -4.2 +1.0 -0.5 -2.3 N.D.
carbon source Ying_sugar7 rep A; time point 2 +1.4 -1.0 -0.4 -6.0 N.D.
carbon source carbon source D-Glucose 0.25mM +0.6 -0.3 +0.2 -6.1 N.D.
carbon source Ying_OrganicAcid7 rep B; time point 3 +1.0 -1.2 +0.7 -5.4 N.D.
carbon source Ying_sugar7 rep B; time point 2 +1.4 -0.9 -0.3 -4.5 N.D.
carbon source Ying_OrganicAcid7 rep C; time point 3 +1.1 -1.1 +0.6 -4.8 N.D.
stress octanol 0.08 vol% -4.5 +1.1 +1.5 +0.1 N.D.
carbon source Glycolic Acid (C) +3.0 -1.6 -1.6 -1.5 N.D.
stress LB Anaerobic with Cefoxitin 0.000003125 mM +6.6 +0.1 +0.6 -0.3 N.D.
stress Cephalothin 0.01 mg/ml +4.0 -1.1 +2.4 +1.9 N.D.
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +6.3 -0.2 +1.0 +1.4 N.D.
stress LB Anaerobic with Cephalothin 0.0000078125 mM +4.3 +0.2 +2.6 +1.5 N.D.
stress LB Anaerobic with Amoxicillin 0.0000078125 mM +7.5 +0.7 +1.2 +0.8 N.D.
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