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Protein
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Fitness for 5 genes in
Escherichia coli BW25113
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
trpT and hdfR are separated by 95 nucleotides
hdfR and yifE are separated by 118 nucleotides
yifE and yifB are separated by 24 nucleotides
yifB and ilvL are separated by 352 nucleotides
b3761: trpT - tRNA-Trp (NCBI), at 3,944,980 to 3,945,055
trpT
b4480: hdfR - transcriptional regulator HdfR (NCBI), at 3,945,151 to 3,945,990
hdfR
b3764: yifE - hypothetical protein (NCBI), at 3,946,109 to 3,946,447
yifE
b3765: yifB - predicted bifunctional enzyme and transcriptional regulator (RefSeq), at 3,946,472 to 3,947,992
yifB
b3766: ilvL - ilvG operon leader peptide (NCBI), at 3,948,345 to 3,948,443
ilvL
Group
Condition
b3761
b4480
b3764
b3765
b3766
trpT
hdfR
yifE
yifB
ilvL
carbon source
carbon source D-Mannose 0.5mM
-5.9
-1.6
-3.8
-0.5
-0.6
stress
octanol 0.08 vol%
-2.0
-4.6
-3.9
-0.5
-1.1
micoeukaryotes
Tetrahymena sp., Mixed culture-1
-6.2
-2.8
-1.3
+0.0
-0.3
carbon source
Ying_sugar7 rep A; time point 3
-1.5
-4.3
-4.1
-0.3
-0.1
stress
undecene 5 vol%
-0.5
-3.8
-5.2
-0.4
-0.3
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-3.2
-2.2
-3.2
-0.4
-0.8
stress
2,4-Dinitrophenol 2 mM
-0.4
-4.7
-4.1
-0.3
-0.3
carbon source
Ying_sugar7 rep B; time point 3
-1.9
-4.0
-3.8
-0.2
+0.4
nitrogen source
Putrescine (N)
+0.2
-4.2
-4.2
-0.4
-0.8
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
-1.0
-3.8
-3.9
-0.4
-0.1
stress
undecene 5 vol%
-0.4
-4.4
-3.7
-0.4
-0.1
carbon source
Glycolic Acid (C)
-1.4
-3.3
-4.0
-0.2
+0.2
nitrogen source
L-Serine (N)
-0.6
-2.7
-4.0
-0.0
-1.3
carbon source
Glycolic Acid (C)
-0.6
-3.7
-4.5
-0.1
+0.4
nitrogen source
Putrescine (N)
+0.4
-3.8
-4.8
-0.3
-0.0
carbon source
carbon source D-Mannose 1mM
+1.0
-1.5
-5.7
-0.5
+0.4
msc
22hr_mediaonly_rep1_lowinoculum
-1.7
+3.6
+3.8
+0.2
-0.5
stress
LB Anaerobic with Ampicillin 0.00000195313 mM
-0.6
+3.5
+3.6
-0.3
-0.6
motility
inner cut, LB soft agar motility assay
-0.2
+4.1
+3.9
+0.2
-1.0
motility
inner cut, LB soft agar motility assay
-1.4
+3.9
+4.3
+0.4
-0.0
motility
inner cut, LB soft agar motility assay
-1.6
+4.4
+4.6
+0.6
-0.3
motility
inner cut, LB soft agar motility assay
+0.6
+4.0
+3.7
+0.3
-0.5
msc
22hr_MSC_rep1_highinoculum
+0.0
+4.4
+4.2
+0.4
-0.9
msc
22hr_mediaonly_rep2_lowinoculum
-0.2
+4.7
+4.9
+0.2
-0.2
motility
outer cut, LB soft agar motility assay
-2.9
+6.0
+6.3
+1.0
-0.6
motility
outer cut, LB soft agar motility assay
-1.7
+5.3
+5.4
+0.6
+0.6
msc
22hr_MSC_rep2_highinoculum
-1.0
+5.5
+5.4
+0.4
-0.2
msc
22hr_MSC_rep1_lowinoculum
-0.9
+5.3
+5.1
+0.4
+0.3
motility
outer cut, LB soft agar motility assay
-1.0
+5.8
+5.7
+0.8
+0.6
msc
22hr_MSC_rep2_lowinoculum
-0.2
+5.8
+5.7
+0.6
-0.0
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