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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
asnA and yieM are separated by 4 nucleotides
yieM and yieN overlap by 7 nucleotides
yieN and trkD are separated by 222 nucleotides
trkD and rbsD are separated by 166 nucleotides
b3744: asnA - asparagine synthetase AsnA (NCBI), at 3,925,178 to 3,926,170
asnA
b3745: yieM - predicted von Willibrand factor containing protein (NCBI), at 3,926,175 to 3,927,626
yieM
b3746: yieN - putative 2-component regulator (VIMSS), at 3,927,620 to 3,929,116
yieN
b3747: trkD - potassium transporter (NCBI), at 3,929,339 to 3,931,207
trkD
b3748: rbsD - D-ribose pyranase [EC:5.4.99.62]
(from data)
, at 3,931,374 to 3,931,793
rbsD
Group
Condition
b3744
b3745
b3746
b3747
b3748
asnA
yieM
yieN
trkD
rbsD
carbon source
D-Ribose (C)
-0.1
+0.1
-0.0
-0.0
-3.1
carbon source
D-Ribose (C)
+0.0
+0.1
-0.1
-0.1
-3.0
stress
LB Anaerobic with Chloramphenicol 0.00000390625 mM
+0.1
-1.4
-1.6
-0.0
+0.1
stress
LB Anaerobic with Chloramphenicol 0.00000390625 mM
+0.1
-1.2
-1.4
-0.1
-0.0
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
-0.0
-1.5
-1.2
+0.1
+0.1
stress
sodium fluoride 100 mM
-0.1
-1.3
-1.0
+0.1
+0.0
carbon source
Glycolic Acid (C)
+0.2
-0.9
-1.2
-0.3
-0.0
motility
outer cut, LB soft agar motility assay
+0.2
-0.5
-0.4
+0.2
-1.1
stress
LB Aerobic with Irgasan 0.0000003125 mM
+0.2
-0.7
-0.5
+0.6
+0.3
mzaabcde
mzaABCDE
-1.1
+0.4
+0.4
+0.1
+0.4
sir2-hera
SIR2-HerA
-1.1
+0.4
+0.2
+0.2
+0.5
retron_ec_48_control
retron_Ec_48_control
-1.0
+0.4
+0.4
+0.2
+0.4
retron_ec_48_control
retron_Ec_48_control
-1.2
+0.4
+0.3
+0.2
+0.5
drt_type_3
DRT_type_3
-0.9
+0.4
+0.4
+0.1
+0.6
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.4
+0.6
+0.6
-0.3
-0.5
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.3
+0.7
+0.8
-0.3
-0.3
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.2
+0.8
+0.7
-0.1
-0.6
stress
M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM
-0.1
+1.0
+0.9
-0.1
+0.1
stress
LB Anaerobic with Hygromycin B 0.00025 mM
+0.1
+1.0
+0.9
+0.0
-0.1
stress
LB Anaerobic with Hygromycin B 0.00025 mM
+0.0
+1.1
+1.1
-0.1
+0.1
m9 anaerobic no stress control
M9 anaerobic no stress control
+0.1
+1.1
+1.1
-0.0
+0.1
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
+0.4
+1.0
+0.8
+0.2
+0.1
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.3
+1.2
+1.2
-0.2
-0.0
stress
LB Anaerobic with Nitrofurantoin 0.000015625 mM
+0.2
+1.1
+1.0
-0.0
+0.3
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.1
+1.5
+1.3
-0.1
+0.0
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.0
+1.4
+1.3
-0.1
+0.2
stress
Ciprofloxacin Hydrochloride 0.0003 mM
+0.7
+0.7
+0.6
+0.6
+0.2
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.4
+1.5
+1.4
-0.3
+0.1
stress
Ciprofloxacin Hydrochloride 0.000156 mM
+0.8
+0.6
+0.5
+0.6
+0.6
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.4
+1.5
+1.6
+0.5
+0.1
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