Fitness for 5 genes in Escherichia coli BW25113

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500 ntatpB and atpI are separated by 8 nucleotidesatpI and gidB are separated by 616 nucleotidesgidB and gidA are separated by 63 nucleotidesgidA and mioC are separated by 378 nucleotides b3738: atpB - F0F1 ATP synthase subunit A (NCBI), at 3,919,259 to 3,920,074 atpB b3739: atpI - membrane-bound ATP synthase, dispensable protein, affects expression of atpB (VIMSS), at 3,920,083 to 3,920,463 atpI b3740: gidB - glucose-inhibited division protein B (NCBI), at 3,921,080 to 3,921,703 gidB b3741: gidA - glucose-inhibited division protein A (NCBI), at 3,921,767 to 3,923,656 gidA b3742: mioC - flavodoxin (NCBI), at 3,924,035 to 3,924,478 mioC
Group Condition b3738 b3739 b3740 b3741 b3742
atpB atpI gidB gidA mioC
stress Bacitracin 1 mg/ml N.D. -0.3 -3.1 -2.3 -2.9
stress 2,4-Dinitrophenol 2 mM N.D. -0.5 -0.6 -4.5 -2.4
stress 2,4-Dinitrophenol 2 mM N.D. -0.6 -0.7 -4.0 -2.2
stress Cobalt chloride 0.32 mM N.D. -0.3 -2.3 -2.8 -0.8
sulfur source M63 no sulfur + 5 mM sulfate N.D. -0.1 -0.9 -4.8 -0.0
phage T7_phage 0.00015625 MOI TC2 N.D. -0.2 -0.1 -2.9 -2.6
sulfur source M63 no sulfur + 5 mM sulfate N.D. -0.2 -0.7 -5.1 +0.1
sulfur source M63 no sulfur + 5 mM L-cys N.D. -0.2 -0.9 -4.9 +0.3
sulfur source M63 no sulfur + 5 mM L-cys N.D. -0.2 -0.7 -4.6 -0.1
phage TP4 N.D. -0.1 -0.4 -1.3 -3.7
stress Nickel (II) chloride 1 mM N.D. +0.2 -1.6 -2.6 -1.4
phage Phage_N4_cage_3_mouse_1_Cecum_40 hr N.D. +0.0 -0.5 -4.4 -0.3
phage Phage_N4_cage_3_mice _Feces_40 hr N.D. -0.1 -0.3 -4.5 -0.2
phage Δdbn_10 N.D. -0.8 +0.2 -1.9 -2.3
stress LB Anaerobic with Cephalothin 0.0000078125 mM N.D. -1.0 +0.6 -0.1 -4.2
phage T7_phage 0.0015625 MOI TC1 N.D. +0.3 -0.4 -2.2 -2.4
control PBS_cage_2_mouse_2_Cecum_40 hr N.D. +0.3 -0.8 -3.4 -0.7
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. +0.1 -3.8 -0.9 +0.7
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. +0.4 -3.7 -0.9 +0.5
carbon source Dodecanol and Rhamnolipid (C) N.D. +0.3 -0.3 -4.5 +1.0
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM N.D. -1.2 +1.1 +1.4 -3.8
stress toluene 2.5 vol% N.D. -0.6 -0.8 -3.3 +2.3
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM N.D. -0.8 +1.3 +1.6 -4.0
carbon source acetate (C) N.D. +0.4 +1.4 -3.5 +0.5
carbon source acetate (C) N.D. +0.2 +1.6 -3.3 +0.3
carbon source Glycolic Acid (C) N.D. +0.2 +3.1 -2.9 -1.3
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM N.D. -0.5 +1.6 +1.7 -3.3
carbon source Glycolic Acid (C) N.D. +0.2 +2.8 -2.5 -0.7
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM N.D. -0.6 +1.9 +3.3 -2.8
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. -1.7 +0.9 +0.8 +2.1
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