Fitness for 5 genes in Escherichia coli BW25113

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500 nttnaC and tnaA are separated by 220 nucleotidestnaA and tnaB are separated by 90 nucleotidestnaB and mdtL are separated by 131 nucleotidesmdtL and yidZ overlap by 26 nucleotides b3707: tnaC - tryptophanase leader peptide (NCBI), at 3,886,458 to 3,886,532 tnaC b3708: tnaA - tryptophanase (VIMSS), at 3,886,753 to 3,888,168 tnaA b3709: tnaB - tryptophan transporter of low affinity (NCBI), at 3,888,259 to 3,889,506 tnaB b3710: mdtL - multidrug efflux system protein (NCBI), at 3,889,638 to 3,890,813 mdtL b3711: yidZ - predicted DNA-binding transcriptional regulator (NCBI), at 3,890,788 to 3,891,747 yidZ
Group Condition b3707 b3708 b3709 b3710 b3711
tnaC tnaA tnaB mdtL yidZ
carbon source Ying_AminoAcid20 rep A; time point 3 N.D. -1.6 -1.0 +0.1 -0.1
carbon source Ying_AminoAcid20 rep B; time point 3 N.D. -1.3 -0.9 +0.1 -0.1
carbon source Ying_AminoAcid20 rep C; time point 3 N.D. -1.3 -0.9 +0.2 +0.1
carbon source Ying_AminoAcid20 rep A; time point 2 N.D. -1.1 -0.8 +0.2 +0.1
carbon source Ying_AminoAcid20 rep C; time point 2 N.D. -0.9 -0.6 +0.2 -0.0
carbon source Ying_AminoAcid20 rep B; time point 2 N.D. -1.0 -0.6 +0.2 +0.0
phage Δdbn_10 N.D. -0.1 +0.1 -0.1 -0.9
stress Trimethoprim 0.0025 mM N.D. -0.0 -0.0 +0.1 -1.1
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr N.D. +0.2 +0.2 -0.6 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. +0.6 -0.0 -0.6 -0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. +0.5 -0.4 -0.4 +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. +0.5 -0.1 -0.1 -0.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. +0.9 -0.2 -0.3 -0.5
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. +0.7 +0.0 -0.2 -0.5
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. +1.1 +0.0 -0.4 -0.4
motility outer cut, LB soft agar motility assay N.D. -0.2 +0.1 +0.3 +0.6
stress LB Anaerobic with Cefoxitin 0.000003125 mM N.D. +0.7 +0.4 -0.1 -0.2
stress Nickel (II) chloride 1.5 mM N.D. +0.5 +0.4 -0.1 +0.3
stress Ciprofloxacin Hydrochloride 0.0003 mM N.D. +0.3 +0.4 +0.4 +0.2
micoeukaryotes Tetrahymena sp., Mixed culture-1 N.D. +0.4 +0.2 +0.5 +0.2
phage RB68 N.D. +0.2 +0.4 +0.3 +0.5
carbon source D-Ribose (C) N.D. +0.6 +0.4 +0.3 +0.1
carbon source Glycolic Acid (C) N.D. +0.8 +0.6 +0.2 -0.1
carbon source Glycolic Acid (C) N.D. +0.7 +0.5 +0.2 -0.0
stress Nickel (II) chloride 1.5 mM N.D. +0.6 +0.4 +0.0 +0.4
stress octanol 0.08 vol% N.D. +0.3 +0.3 +0.5 +0.4
stress Nickel (II) chloride 1.5 mM N.D. +0.5 +0.4 +0.2 +0.4
phage Ste41 N.D. +0.2 +0.4 +0.8 +0.3
stress Ciprofloxacin Hydrochloride 0.000156 mM N.D. +0.5 +0.5 +0.5 +0.3
stress Ciprofloxacin Hydrochloride 0.0003 mM N.D. +0.6 +0.5 +0.7 +0.5
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