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Cofit
Protein
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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
tnaC and tnaA are separated by 220 nucleotides
tnaA and tnaB are separated by 90 nucleotides
tnaB and mdtL are separated by 131 nucleotides
mdtL and yidZ overlap by 26 nucleotides
b3707: tnaC - tryptophanase leader peptide (NCBI), at 3,886,458 to 3,886,532
tnaC
b3708: tnaA - tryptophanase (VIMSS), at 3,886,753 to 3,888,168
tnaA
b3709: tnaB - tryptophan transporter of low affinity (NCBI), at 3,888,259 to 3,889,506
tnaB
b3710: mdtL - multidrug efflux system protein (NCBI), at 3,889,638 to 3,890,813
mdtL
b3711: yidZ - predicted DNA-binding transcriptional regulator (NCBI), at 3,890,788 to 3,891,747
yidZ
Group
Condition
b3707
b3708
b3709
b3710
b3711
tnaC
tnaA
tnaB
mdtL
yidZ
carbon source
Ying_AminoAcid20 rep A; time point 3
N.D.
-1.6
-1.0
+0.1
-0.1
carbon source
Ying_AminoAcid20 rep B; time point 3
N.D.
-1.3
-0.9
+0.1
-0.1
carbon source
Ying_AminoAcid20 rep C; time point 3
N.D.
-1.3
-0.9
+0.2
+0.1
carbon source
Ying_AminoAcid20 rep A; time point 2
N.D.
-1.1
-0.8
+0.2
+0.1
carbon source
Ying_AminoAcid20 rep C; time point 2
N.D.
-0.9
-0.6
+0.2
-0.0
carbon source
Ying_AminoAcid20 rep B; time point 2
N.D.
-1.0
-0.6
+0.2
+0.0
phage
Δdbn_10
N.D.
-0.1
+0.1
-0.1
-0.9
stress
Trimethoprim 0.0025 mM
N.D.
-0.0
-0.0
+0.1
-1.1
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
N.D.
+0.2
+0.2
-0.6
-0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
N.D.
+0.6
-0.0
-0.6
-0.3
plasmid transformation
mariner vector transformation; liquid outgrowth assay
N.D.
+0.5
-0.4
-0.4
+0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
N.D.
+0.5
-0.1
-0.1
-0.4
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
N.D.
+0.9
-0.2
-0.3
-0.5
plasmid transformation
mariner vector transformation; liquid outgrowth assay
N.D.
+0.7
+0.0
-0.2
-0.5
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
N.D.
+1.1
+0.0
-0.4
-0.4
motility
outer cut, LB soft agar motility assay
N.D.
-0.2
+0.1
+0.3
+0.6
stress
LB Anaerobic with Cefoxitin 0.000003125 mM
N.D.
+0.7
+0.4
-0.1
-0.2
stress
Nickel (II) chloride 1.5 mM
N.D.
+0.5
+0.4
-0.1
+0.3
stress
Ciprofloxacin Hydrochloride 0.0003 mM
N.D.
+0.3
+0.4
+0.4
+0.2
micoeukaryotes
Tetrahymena sp., Mixed culture-1
N.D.
+0.4
+0.2
+0.5
+0.2
phage
RB68
N.D.
+0.2
+0.4
+0.3
+0.5
carbon source
D-Ribose (C)
N.D.
+0.6
+0.4
+0.3
+0.1
carbon source
Glycolic Acid (C)
N.D.
+0.8
+0.6
+0.2
-0.1
carbon source
Glycolic Acid (C)
N.D.
+0.7
+0.5
+0.2
-0.0
stress
Nickel (II) chloride 1.5 mM
N.D.
+0.6
+0.4
+0.0
+0.4
stress
octanol 0.08 vol%
N.D.
+0.3
+0.3
+0.5
+0.4
stress
Nickel (II) chloride 1.5 mM
N.D.
+0.5
+0.4
+0.2
+0.4
phage
Ste41
N.D.
+0.2
+0.4
+0.8
+0.3
stress
Ciprofloxacin Hydrochloride 0.000156 mM
N.D.
+0.5
+0.5
+0.5
+0.3
stress
Ciprofloxacin Hydrochloride 0.0003 mM
N.D.
+0.6
+0.5
+0.7
+0.5
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