Fitness for 5 genes in Escherichia coli BW25113

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500 ntibpB and ibpA are separated by 111 nucleotidesibpA and yidQ are separated by 305 nucleotidesyidQ and yidR are separated by 1 nucleotidesyidR and yidS are separated by 67 nucleotides b3686: ibpB - heat shock chaperone (NCBI), at 3,864,492 to 3,864,920 ibpB b3687: ibpA - heat shock chaperone (NCBI), at 3,865,032 to 3,865,445 ibpA b3688: yidQ - orf, hypothetical protein (VIMSS), at 3,865,751 to 3,866,083 yidQ b3689: yidR - conserved protein (NCBI), at 3,866,085 to 3,867,332 yidR b3690: yidS - orf, hypothetical protein (VIMSS), at 3,867,400 to 3,868,464 yidS
Group Condition b3686 b3687 b3688 b3689 b3690
ibpB ibpA yidQ yidR yidS
stress LB Aerobic with Ramoplanin 0.000125 mM -0.1 -3.6 +0.3 +0.3 -0.0
stress LB Aerobic with Ramoplanin 0.000125 mM -0.4 -2.9 +0.5 +0.2 -0.0
phage Ste41 -0.9 +0.2 -1.3 -0.4 -0.1
stress Cisplatin 0.1 mg/ml -1.1 -0.4 -0.2 +0.2 -0.1
stress Bacitracin 1 mg/ml -1.6 -2.2 +1.5 +0.3 +0.3
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.9 -2.7 +2.1 +0.1 +0.0
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.7 -2.7 +2.0 +0.2 +0.1
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.5 -2.5 +1.8 +0.3 -0.2
stress Bacitracin 0.5 mg/ml -0.5 -1.6 +0.8 +0.1 +0.4
stress Chloramphenicol 0.001 mg/ml -0.8 -0.8 +0.5 +0.2 +0.1
stress LB Aerobic with Doxycycline hyclate 0.00000625 mM -0.7 -0.6 +0.4 +0.2 +0.1
stress Chloramphenicol 0.0025 mg/ml -1.1 -0.1 +0.6 +0.1 -0.1
stress undecene 5 vol% -0.4 -0.8 +0.6 +0.1 -0.1
stress limonene 2.5 vol% -0.4 -0.7 +0.7 +0.0 -0.2
stress LB Anaerobic with Spiramycin 0.000125 mM -0.7 -0.9 +1.2 +0.2 +0.0
stress LB Aerobic with Doxycycline hyclate 0.0000015625 mM -0.2 -0.7 +0.7 +0.1 +0.0
stress Nalidixic 0.06 mM -0.1 -0.8 +1.0 +0.1 -0.1
stress Benzalkonium Chloride 0.004 mg/ml -1.1 -0.9 +1.7 +0.2 +0.2
stress Nalidixic 0.06 mM -0.2 -0.8 +1.3 +0.1 -0.1
stress LB Anaerobic with Spiramycin 0.000125 mM -0.5 -0.9 +1.2 +0.3 +0.3
stress Fusidic 0.03 mg/ml -0.4 -0.2 +1.0 -0.1 +0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.6 +0.3 +0.6 +0.1 +0.5
stress LB Anaerobic with Doxycycline hyclate 0.000000390625 mM -0.0 -0.3 +1.4 +0.0 -0.1
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.6 +0.6 +0.3 +0.5 +0.3
mzaabcde mzaABCDE +0.2 +0.3 +0.8 -0.3 +0.3
stress Fusidic 0.1 mg/ml -0.2 -0.4 +1.6 +0.3 +0.2
carbon source L-Malic (C) +0.8 -0.1 +0.5 +0.1 +0.4
nitrogen source L-Arginine (N) +1.3 -0.1 +0.1 +0.2 +0.1
carbon source a-Ketoglutaric (C) +1.1 +0.2 +0.4 +0.1 +0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.8 +0.1 +0.7 +0.3 +0.2
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