Fitness for 5 genes in Escherichia coli BW25113

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500 ntemrD and yidF are separated by 7 nucleotidesyidF and yidG overlap by 4 nucleotidesyidG and yidH overlap by 11 nucleotidesyidH and yidI are separated by 107 nucleotides b3673: emrD - 2-module integral membrane pump; multidrug resistance (VIMSS), at 3,851,945 to 3,853,129 emrD b3674: yidF - predicted DNA-binding transcriptional regulator (NCBI), at 3,853,137 to 3,853,634 yidF b3675: yidG - predicted inner membrane protein (NCBI), at 3,853,631 to 3,853,993 yidG b3676: yidH - conserved inner membrane protein (NCBI), at 3,853,983 to 3,854,330 yidH b3677: yidI - predicted inner membrane protein (NCBI), at 3,854,438 to 3,854,887 yidI
Group Condition b3673 b3674 b3675 b3676 b3677
emrD yidF yidG yidH yidI
carbon source acetate (C) -0.1 -0.1 -3.5 -2.4 -0.9
carbon source acetate (C) -0.2 -0.0 -2.4 -3.6 -0.4
carbon source succinate (C) -0.3 -0.1 -2.2 -2.2 -0.0
carbon source D-Ribose (C) -0.2 +0.1 -2.2 -1.3 -0.3
carbon source D-Ribose (C) -0.1 +0.1 -1.8 -1.4 -0.5
carbon source succinate (C) -0.4 -0.1 -1.3 -1.6 -0.3
carbon source Glycolic Acid (C) -1.0 -0.1 -1.5 -1.6 +0.6
carbon source D-Glucosamine Hydrochloride (C) -0.5 +0.1 -1.4 -1.2 -0.3
carbon source L-Malic (C) -0.3 +0.0 -1.4 -1.0 -0.4
carbon source Acetate (C) -0.1 +0.1 -1.0 -1.3 -0.6
carbon source Glycolic Acid (C) -1.0 -0.1 -1.6 -0.9 +0.6
M9 Acetate 0.5% aerobic -0.5 -0.1 -1.1 -1.5 +0.3
nitrogen source Putrescine (N) -0.6 -0.3 -0.9 -0.5 -0.5
carbon source D-Glucosamine Hydrochloride (C) -0.3 +0.0 -1.4 -1.0 -0.0
carbon source L-Malic (C) -0.2 +0.1 -0.9 -1.1 -0.5
phage T7_phage 0.0015625 MOI TC1 -0.9 -0.3 +0.1 -0.8 +0.6
phage Ste41 +0.9 +0.5 -1.0 -1.6 -0.0
stress LB Aerobic with Nitrofurantoin 0.00003125 mM -1.7 -0.2 +0.3 +0.2 +0.5
phage T7_phage 0.00015625 MOI TC4 -0.6 -0.4 +0.2 -0.7 +1.0
motility outer cut, LB soft agar motility assay +0.9 +0.2 +0.4 -0.6 -0.8
phage Ste41 +1.1 +0.1 -0.4 -1.0 +0.5
stress LB Anaerobic with Trimethoprim 0.00000125 mM +1.6 +0.2 -0.4 +0.1 -0.3
stress LB Anaerobic with Cephalothin 0.0000078125 mM +2.0 +0.1 -0.3 +0.1 -0.1
stress LB Anaerobic with Ampicillin 0.00000195313 mM +1.8 -0.2 +0.2 +0.6 -0.1
stress Benzalkonium Chloride 0.004 mg/ml +1.8 +0.4 +0.0 +0.9 -0.6
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.3 +0.3 +0.8 +0.6 +0.7
stress Trimethoprim 0.0025 mM +2.5 +0.0 +0.2 +0.3 -0.2
stress LB Anaerobic with Trimethoprim 0.0000003125 mM +3.2 +0.1 -0.1 -0.1 -0.0
stress Trimethoprim 0.00125 mM +2.2 +0.3 +0.2 +0.4 -0.0
stress Ciprofloxacin Hydrochloride 0.0003 mM +1.0 +0.6 +0.1 +1.2 +0.7
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