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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
gltS and yicE are separated by 279 nucleotides
yicE and yicH are separated by 120 nucleotides
yicH and yicI are separated by 52 nucleotides
yicI and yicJ are separated by 9 nucleotides
b3653: gltS - glutamate transporter (NCBI), at 3,825,483 to 3,826,688
gltS
b3654: yicE - predicted transporter (NCBI), at 3,826,968 to 3,828,359
yicE
b3655: yicH - hypothetical protein (NCBI), at 3,828,480 to 3,830,189
yicH
b3656: yicI - predicted alpha-glucosidase (NCBI), at 3,830,242 to 3,832,560
yicI
b3657: yicJ - putative permease (VIMSS), at 3,832,570 to 3,833,952
yicJ
Group
Condition
b3653
b3654
b3655
b3656
b3657
gltS
yicE
yicH
yicI
yicJ
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.4
-0.4
-2.0
-0.3
+0.1
phage
T7_phage 0.0015625 MOI TC2
-0.4
-0.5
-0.0
-0.3
-0.8
stress
octanol 0.08 vol%
-0.6
-0.1
-0.1
-0.3
-0.6
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.2
-0.0
-1.1
-0.4
-0.0
phage
Ste41
-0.3
-0.1
-0.2
-0.5
-0.3
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
-0.5
-0.1
-0.6
-0.0
-0.2
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.0
-0.9
-0.6
+0.3
-0.1
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
-0.2
-0.2
-0.8
-0.0
-0.1
control
PBS_cage_1_mouse_2_Cecum_40 hr
-0.3
-0.2
+0.0
-0.3
-0.4
predation
6 hr rep2_HD100_10fold_dilution_grewON
-0.5
-0.4
-0.2
-0.3
+0.4
predation
6 hr rep1_HD100_10fold_dilution_grewON
-0.7
-0.3
-0.2
+0.3
-0.0
stress
LB Anaerobic with Ampicillin 0.00000195313 mM
-0.2
-0.1
-0.2
-0.4
+0.4
phage
Lambda_ci1857
-0.6
+0.2
-0.1
-0.2
+0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.8
+0.6
+0.1
-0.0
-0.3
micoeukaryotes
Tetrahymena sp., Mixed culture-1
-0.4
+0.2
-0.4
+0.2
+0.0
stress
Bacitracin 1 mg/ml
+0.6
-0.5
+0.1
-0.3
+0.1
stress
Cisplatin 0.1 mg/ml
+0.5
-0.4
-0.1
-0.1
+0.1
carbon source
carbon source D-Glucose 0.25mM
-0.5
+0.2
+0.4
-0.0
+0.1
phage
T7_phage 0.00015625 MOI TC4
+0.3
-0.1
+0.1
+0.4
-0.5
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
+0.7
-0.3
-0.1
+0.1
-0.1
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
-0.4
-0.2
+0.4
+0.1
+0.4
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.3
+0.4
+0.1
-0.3
+0.1
stress
Benzalkonium Chloride 0.004 mg/ml
+0.8
-0.4
+0.2
+0.1
+0.1
carbon source
carbon source D-Mannose 1mM
+0.2
-0.1
+0.8
+0.0
+0.2
carbon source
carbon source D-Glucose 20mM
+0.0
+0.4
+1.4
+0.0
-0.2
carbon source
carbon source D-Glucose 20mM
+0.0
+0.3
+1.4
-0.2
+0.1
carbon source
carbon source D-Mannose 5mM
+0.4
-0.2
+1.5
+0.1
+0.0
carbon source
carbon source D-Mannose 20mM
-0.3
+0.1
+2.2
-0.0
+0.0
carbon source
carbon source D-Mannose 5mM
+0.2
+0.2
+1.5
+0.3
+0.0
carbon source
carbon source D-Mannose 20mM
+0.1
+0.1
+2.0
+0.2
+0.1
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