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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
spoT and trmH are separated by 6 nucleotides
trmH and recG are separated by 5 nucleotides
recG and gltS are separated by 168 nucleotides
gltS and yicE are separated by 279 nucleotides
b3650: spoT - bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (NCBI), at 3,820,423 to 3,822,531
spoT
b3651: trmH - tRNA (Guanosine-2'-O-)-methyltransferase (NCBI), at 3,822,538 to 3,823,227
trmH
b3652: recG - ATP-dependent DNA helicase (NCBI), at 3,823,233 to 3,825,314
recG
b3653: gltS - glutamate transporter (NCBI), at 3,825,483 to 3,826,688
gltS
b3654: yicE - predicted transporter (NCBI), at 3,826,968 to 3,828,359
yicE
Group
Condition
b3650
b3651
b3652
b3653
b3654
spoT
trmH
recG
gltS
yicE
stress
LB + carbenicillin regrowth after 48hr
-1.3
-1.2
-4.9
-0.0
-0.1
stress
LB + carbenicillin regrowth after 48hr
-0.9
-1.1
-5.2
+0.0
-0.1
phage
Δdbn_10
-0.5
-1.4
-3.8
-0.3
-0.0
TN_15CS_warm37C_5dbl_rep3
-0.9
-1.0
-3.9
-0.1
-0.1
TN_15CS_warm37C_5dbl_rep2
-0.8
-1.0
-3.6
-0.3
-0.0
TN_18CS_warm37C_5dbl_rep3
-0.8
-1.0
-3.7
-0.1
+0.0
TN_18CS_warm37C_5dbl_rep1
-0.9
-1.0
-3.6
+0.0
+0.1
TN_18CS_warm37C_5dbl_rep2
-0.7
-1.0
-3.5
+0.0
-0.1
TN_15CS_warm37C_5dbl_rep1
-0.9
-1.0
-3.2
-0.1
-0.0
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
-0.6
-1.0
-2.7
-0.1
-0.6
stress
Cisplatin 0.1 mg/ml
-0.1
-1.3
-3.3
-0.0
-0.1
mzaabcde
mzaABCDE
+0.0
-0.7
-3.5
-0.3
-0.1
stress
LB Anaerobic with Furaltadone 0.000015625 mM
-1.0
-0.9
-2.4
-0.2
-0.0
mzaabcde
mzaABCDE
+0.3
-0.8
-3.7
-0.2
-0.2
carbon source
Ying_Nucleobase14 rep C; time point 3
-3.4
-0.0
-0.7
-0.1
-0.2
carbon source
D-Galacturonic Acid (C) MOPS
-2.9
-0.5
-1.1
-0.1
+0.1
stress
LB Anaerobic with Nitrofurantoin 0.000015625 mM
-1.5
-0.4
-2.0
-0.3
-0.1
carbon source
D-Glucose-6-Phosphate (C)
-3.6
-0.3
-0.6
+0.0
+0.1
mzaabcde
mzaABCDE
+0.4
-1.0
-3.6
+0.1
-0.2
TN_15CS_15C_10dbl_rep3
-0.6
-0.7
-2.7
-0.2
-0.0
protonated/deuterated
deuterated glucose in D2O
-2.7
-0.4
-1.2
+0.0
+0.0
phage
P2_phage_0.5625_MOI
-0.4
-0.7
-2.9
-0.3
+0.1
phage
Lambda_ci1857
-0.8
-0.5
-2.5
-0.6
+0.2
stress
limonene 2.5 vol%
-0.2
-0.8
-3.0
+0.1
-0.1
nitrogen source
Gly-DL-Asp (N)
-3.2
-0.4
-0.5
-0.0
+0.1
carbon source
Ying_Nucleobase14 rep B; time point 3
-3.1
+0.1
-0.7
+0.1
-0.3
stress
Cisplatin 0.1 mg/ml
+1.2
-1.7
-2.1
+0.5
-0.4
stress
methylglyoxal 0.008 vol%
+2.2
-1.2
-2.9
+0.3
-0.2
phage
T7_phage 0.0015625 MOI TC2
+0.4
+1.3
+2.5
-0.4
-0.5
phage
T7_phage 0.00015625 MOI TC3
+0.3
+0.9
+2.7
+0.0
-0.3
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